Automated F(C)CS analysis of clone # 2960hi

Created on 12/08/2014
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Gene and protein information

Clone # 2960hi
Gene symbol: F8A2
Description: coagulation factor VIII-associated 2
Molecular weight (with FP): 66.1 kDa
Localisation: homogeneous nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
2960hi_img_ch1.png2960hi_img_ch2.png


Point 1, intensity traces and correlation functions
2960hi_itr_1.png2960hi_cor_1.png

Point 2, intensity traces and correlation functions
2960hi_itr_2.png2960hi_cor_2.png

Point 3, intensity traces and correlation functions
2960hi_itr_3.png2960hi_cor_3.png

Point 4, intensity traces and correlation functions
2960hi_itr_4.png2960hi_cor_4.png

Point 5, intensity traces and correlation functions
2960hi_itr_5.png2960hi_cor_5.png

Point 6, intensity traces and correlation functions
2960hi_itr_6.png2960hi_cor_6.png

Sessions

1 20130109\U05--V00--2960hi
2 20130111\U01--V01--2960hi

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.891E+2 1.225E+2 8.633E+2 4.094E+2 3.562E+1 3.261E+1 1.428E+2 1.437E+2 5.325E+2 3.145E+2 6.469E+1 6.680E+1
N_corr 1.908E+2 1.217E+2 1.214E+3 7.264E+2 3.885E+1 3.716E+1 1.516E+2 1.571E+2 9.094E+2 5.907E+2 7.840E+1 7.058E+1
c [nM] 1.176E+3 7.617E+2 4.004E+3 1.899E+3 1.831E+2 1.677E+2 8.881E+2 8.936E+2 2.470E+3 1.459E+3 3.326E+2 3.434E+2
c_corr [nM] 1.187E+3 7.566E+2 5.629E+3 3.369E+3 1.997E+2 1.910E+2 9.426E+2 9.773E+2 4.218E+3 2.740E+3 4.030E+2 3.629E+2
ratioG 1.454E-1 8.751E-2 3.133E-1 2.359E-1
ratioG_corr 5.083E-3 5.534E-2 1.182E-1 9.826E-2
f_1 8.822E-1 1.031E-1 3.832E-1 2.418E-1 9.584E-1 1.131E-1 8.569E-1 6.679E-2 6.564E-1 2.904E-1 9.183E-1 9.089E-2
tauD_1 [ms] 2.015E+0 2.219E+0 1.860E+0 4.441E-16 2.162E+0 2.332E+0 1.093E+0 7.491E-1 1.860E+0 0.000E+0 1.019E+0 6.590E-1
D_1 [mum^2/s] 9.997E+0 8.320E+0 6.926E+0 8.882E-16 1.816E+1 1.737E+1 1.288E+1 6.609E+0 6.926E+0 0.000E+0 1.483E+1 5.637E+0
alpha_1 9.985E-1 1.045E-1 9.200E-1 1.110E-16 9.727E-1 2.102E-1 1.021E+0 1.161E-1 9.200E-1 0.000E+0 9.763E-1 1.924E-1
f_2 1.178E-1 1.031E-1 6.168E-1 2.418E-1 4.155E-2 1.131E-1 1.431E-1 6.679E-2 3.436E-1 2.904E-1 8.169E-2 9.089E-2
tauD_2 [ms] 3.330E+1 3.991E+1 5.443E+1 6.699E+1 1.005E+2 8.375E+1 3.682E+1 3.328E+1 2.683E+1 1.890E+1 4.167E+1 1.225E+1
D_2 [mum^2/s] 5.024E-1 2.497E-1 7.567E-1 9.084E-1 1.628E-1 7.280E-2 4.218E-1 2.200E-1 9.689E-1 9.766E-1 3.179E-1 1.077E-1
alpha_2 1.326E+0 3.196E-1 1.331E+0 3.435E-1 1.132E+0 1.635E-1 1.132E+0 1.933E-1 1.113E+0 9.159E-2 1.318E+0 3.702E-1
tauD_m [ms] 5.887E+0 7.629E+0 3.394E+1 5.043E+1 5.203E+0 8.686E+0 6.095E+0 5.671E+0 1.026E+1 1.527E+1 4.085E+0 4.453E+0
D_m [mum^2/s] 8.994E+0 7.901E+0 3.210E+0 1.358E+0 1.786E+1 1.738E+1 1.106E+1 5.823E+0 4.946E+0 1.728E+0 1.371E+1 5.314E+0
R [1/s] 1.116E+1 1.874E+1 9.285E+0 1.436E+1
CPM [kHz] 9.306E-1 9.059E-1 3.673E-1 3.329E-1 6.684E-1 1.923E-1 2.709E-1 1.836E-1
CPM_corr [kHz] 9.446E-1 9.051E-1 4.466E-1 4.092E-1 6.903E-1 1.918E-1 3.971E-1 2.079E-1
Int [kHz] 1.934E+2 2.314E+2 1.767E+2 5.562E+1 9.229E+1 6.762E+1 1.076E+2 5.991E+1
Int_corr [kHz] 1.986E+2 2.368E+2 2.849E+2 1.007E+2 1.008E+2 7.876E+1 2.415E+2 1.355E+2
# samples 114 114 98 98 43 43 87 87 16 16 10 10
# cells 55

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 1186.717 - sd = 756.575Int_corr [kHz] - Ch1 - Nuc : ave = 198.576 - sd = 236.785r = 0.906 - r^2 = 0.821
f_1 - Ch1 - Nuc : ave = 0.882 - sd = 0.103D_1 [mum^2/s] - Ch1 - Nuc : ave = 9.997 - sd = 8.320r = -0.148 - r^2 = 0.022
f_2 - Ch1 - Nuc : ave = 0.118 - sd = 0.103D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.502 - sd = 0.250r = 0.273 - r^2 = 0.074
CPM_corr [kHz] - Ch1 - Nuc : ave = 0.945 - sd = 0.905D_m [mum^2/s] - Ch1 - Nuc : ave = 8.994 - sd = 7.901r = -0.413 - r^2 = 0.170
c_corr [nM] - Ch1 - Nuc : ave = 1186.717 - sd = 756.575ratioG_corr - Cross - Nuc : ave = 0.005 - sd = 0.055r = -0.628 - r^2 = 0.395
c_corr [nM] - Ch1 - Cyto : ave = 942.567 - sd = 977.331Int_corr [kHz] - Ch1 - Cyto : ave = 100.839 - sd = 78.760r = 0.927 - r^2 = 0.860
f_1 - Ch1 - Cyto : ave = 0.857 - sd = 0.067D_1 [mum^2/s] - Ch1 - Cyto : ave = 12.881 - sd = 6.609r = -0.288 - r^2 = 0.083
f_2 - Ch1 - Cyto : ave = 0.143 - sd = 0.067D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.422 - sd = 0.220r = -0.020 - r^2 = 0.000
CPM_corr [kHz] - Ch1 - Cyto : ave = 0.690 - sd = 0.192D_m [mum^2/s] - Ch1 - Cyto : ave = 11.059 - sd = 5.823r = -0.264 - r^2 = 0.070