Automated F(C)CS analysis of clone # 2851

Created on 12/08/2014
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Gene and protein information

Clone # 2851
Gene symbol: AC004381.6
Description: Putative RNA exonuclease NEF-sp
Molecular weight (with FP): 116.9 kDa
Localisation: homogeneous nucleus, bright nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
2851_img_ch1.png2851_img_ch2.png


Point 1, intensity traces and correlation functions
2851_itr_1.png2851_cor_1.png

Point 2, intensity traces and correlation functions
2851_itr_2.png2851_cor_2.png

Point 3, intensity traces and correlation functions
2851_itr_3.png2851_cor_3.png

Point 4, intensity traces and correlation functions
2851_itr_4.png2851_cor_4.png

Point 5, intensity traces and correlation functions
2851_itr_5.png2851_cor_5.png

Point 6, intensity traces and correlation functions
2851_itr_6.png2851_cor_6.png

Sessions

1 20111123\U01--V00--2851
2 20120125\U00--V00--2851
3 20120216\U00--V00--2851
4 20120307\U02--V00--2851
5 20120316\U00--V00--2851

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.107E+2 9.218E+1 1.125E+3 5.847E+2 7.457E+0 9.633E+0 5.078E+1 3.461E+1 2.247E+2 2.862E+2 2.578E+1 2.647E+1
N_corr 1.551E+2 1.962E+2 1.771E+3 1.142E+3 1.091E+1 1.956E+1 5.808E+1 4.607E+1 2.603E+2 5.087E+2 3.187E+1 3.130E+1
c [nM] 6.883E+2 5.733E+2 5.220E+3 2.712E+3 3.834E+1 4.952E+1 3.158E+2 2.152E+2 1.042E+3 1.327E+3 1.325E+2 1.361E+2
c_corr [nM] 9.647E+2 1.220E+3 8.216E+3 5.295E+3 5.609E+1 1.005E+2 3.612E+2 2.865E+2 1.207E+3 2.359E+3 1.638E+2 1.609E+2
ratioG 6.728E-2 3.987E-2 5.198E-1 2.296E-1
ratioG_corr 3.336E-3 2.306E-2 8.743E-2 4.605E-2
f_1 8.168E-1 1.434E-1 3.661E-1 2.085E-1 9.754E-1 6.856E-2 8.750E-1 6.887E-2 8.122E-1 2.332E-1 9.528E-1 5.979E-2
tauD_1 [ms] 2.598E+0 1.273E+0 1.860E+0 4.441E-16 1.400E+0 1.445E+0 1.510E+0 8.254E-1 1.860E+0 0.000E+0 9.200E-1 4.055E-1
D_1 [mum^2/s] 5.064E+0 2.757E+0 6.926E+0 8.882E-16 1.635E+1 1.577E+1 9.206E+0 5.982E+0 6.926E+0 0.000E+0 1.646E+1 1.011E+1
alpha_1 9.725E-1 9.251E-2 9.200E-1 1.110E-16 8.040E-1 2.081E-1 9.392E-1 9.765E-2 9.200E-1 0.000E+0 7.108E-1 9.109E-2
f_2 1.832E-1 1.434E-1 6.339E-1 2.085E-1 2.456E-2 6.856E-2 1.250E-1 6.887E-2 1.878E-1 2.332E-1 4.722E-2 5.979E-2
tauD_2 [ms] 3.522E+1 2.662E+1 6.888E+1 7.906E+1 1.007E+2 6.411E+1 3.433E+1 3.114E+1 4.285E+1 4.123E+1 3.819E+1 3.607E+1
D_2 [mum^2/s] 3.906E-1 1.702E-1 6.153E-1 7.926E-1 1.608E-1 8.476E-2 4.520E-1 2.125E-1 5.610E-1 5.013E-1 4.727E-1 2.139E-1
alpha_2 1.431E+0 2.848E-1 1.296E+0 3.036E-1 1.108E+0 1.348E-1 1.243E+0 2.522E-1 1.122E+0 1.159E-1 1.212E+0 2.225E-1
tauD_m [ms] 9.438E+0 9.966E+0 4.207E+1 5.125E+1 3.768E+0 7.740E+0 5.358E+0 5.293E+0 1.020E+1 1.849E+1 2.076E+0 1.433E+0
D_m [mum^2/s] 4.056E+0 1.881E+0 3.013E+0 1.143E+0 1.578E+1 1.535E+1 7.928E+0 4.591E+0 5.763E+0 1.452E+0 1.561E+1 9.648E+0
R [1/s] 1.690E+1 1.743E+1 1.358E+1 1.613E+1
CPM [kHz] 1.500E+0 4.900E-1 4.092E-1 3.418E-1 1.432E+0 5.812E-1 1.636E-1 9.518E-2
CPM_corr [kHz] 1.525E+0 4.970E-1 4.557E-1 3.784E-1 1.497E+0 5.936E-1 6.702E-1 4.760E-1
Int [kHz] 1.450E+2 7.079E+1 2.050E+2 5.766E+1 7.042E+1 5.299E+1 4.310E+1 6.330E+1
Int_corr [kHz] 1.886E+2 1.355E+2 3.481E+2 1.135E+2 7.924E+1 5.722E+1 6.839E+1 1.108E+2
# samples 119 119 90 90 70 70 96 96 34 34 31 31
# cells 40

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 964.657 - sd = 1220.241Int_corr [kHz] - Ch1 - Nuc : ave = 188.625 - sd = 135.521r = 0.782 - r^2 = 0.611
f_1 - Ch1 - Nuc : ave = 0.817 - sd = 0.143D_1 [mum^2/s] - Ch1 - Nuc : ave = 5.064 - sd = 2.757r = -0.167 - r^2 = 0.028
f_2 - Ch1 - Nuc : ave = 0.183 - sd = 0.143D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.391 - sd = 0.170r = -0.277 - r^2 = 0.077
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.525 - sd = 0.497D_m [mum^2/s] - Ch1 - Nuc : ave = 4.056 - sd = 1.881r = 0.038 - r^2 = 0.001
c_corr [nM] - Ch1 - Nuc : ave = 964.657 - sd = 1220.241ratioG_corr - Cross - Nuc : ave = 0.003 - sd = 0.023r = 0.610 - r^2 = 0.372
c_corr [nM] - Ch1 - Cyto : ave = 361.183 - sd = 286.506Int_corr [kHz] - Ch1 - Cyto : ave = 79.242 - sd = 57.219r = 0.503 - r^2 = 0.253
f_1 - Ch1 - Cyto : ave = 0.875 - sd = 0.069D_1 [mum^2/s] - Ch1 - Cyto : ave = 9.206 - sd = 5.982r = -0.546 - r^2 = 0.298
f_2 - Ch1 - Cyto : ave = 0.125 - sd = 0.069D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.452 - sd = 0.213r = 0.053 - r^2 = 0.003
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.497 - sd = 0.594D_m [mum^2/s] - Ch1 - Cyto : ave = 7.928 - sd = 4.591r = -0.005 - r^2 = 2.516E-5