Automated F(C)CS analysis of clone # 1368

Created on 12/08/2014
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Gene and protein information

Clone # 1368
Gene symbol: KIAA1967
Description: KIAA1967
Molecular weight (with FP): 129.9 kDa
Localisation: homogeneous nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
1368_img_ch1.png1368_img_ch2.png


Point 1, intensity traces and correlation functions
1368_itr_1.png1368_cor_1.png

Point 2, intensity traces and correlation functions
1368_itr_2.png1368_cor_2.png

Point 3, intensity traces and correlation functions
1368_itr_3.png1368_cor_3.png

Point 4, intensity traces and correlation functions
1368_itr_4.png1368_cor_4.png

Point 5, intensity traces and correlation functions
1368_itr_5.png1368_cor_5.png

Point 6, intensity traces and correlation functions
1368_itr_6.png1368_cor_6.png

Sessions

1 20111125\U03--V01--1368
2 20111130\U01--V01--1368
3 20111201\U01--V01--1368
4 20111207\U03--V01--1368
5 20111216\U03--V00--1368

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 6.457E+1 4.440E+1 1.135E+3 5.638E+2 5.343E+0 7.412E+0 1.944E+1 3.067E+1 2.226E+2 2.357E+2 1.612E+1 2.683E+1
N_corr 7.551E+1 5.456E+1 1.626E+3 8.906E+2 7.393E+0 1.099E+1 2.064E+1 4.771E+1 3.124E+2 4.392E+2 5.244E+1 1.697E+2
c [nM] 4.016E+2 2.761E+2 5.266E+3 2.615E+3 2.747E+1 3.811E+1 1.209E+2 1.907E+2 1.032E+3 1.093E+3 8.288E+1 1.379E+2
c_corr [nM] 4.696E+2 3.393E+2 7.542E+3 4.131E+3 3.801E+1 5.649E+1 1.284E+2 2.967E+2 1.449E+3 2.037E+3 2.696E+2 8.723E+2
ratioG 7.308E-2 5.309E-2 3.034E-1 2.131E-1
ratioG_corr 3.879E-3 4.071E-2 1.912E-1 2.452E-1
f_1 9.310E-1 1.041E-1 4.435E-1 2.258E-1 9.668E-1 7.764E-2 8.797E-1 7.293E-2 6.449E-1 3.462E-1 9.399E-1 1.010E-1
tauD_1 [ms] 4.045E+0 1.269E+0 1.860E+0 2.220E-16 2.709E+0 1.208E+0 1.085E+0 1.326E+0 1.860E+0 0.000E+0 1.839E+0 9.936E-1
D_1 [mum^2/s] 3.095E+0 2.306E+0 6.926E+0 3.553E-15 5.890E+0 7.875E+0 2.105E+1 1.670E+1 6.926E+0 0.000E+0 1.266E+1 1.655E+1
alpha_1 9.695E-1 9.762E-2 9.200E-1 8.882E-16 9.705E-1 2.043E-1 9.713E-1 1.351E-1 9.200E-1 0.000E+0 9.546E-1 2.479E-1
f_2 6.904E-2 1.041E-1 5.565E-1 2.258E-1 3.319E-2 7.764E-2 1.203E-1 7.293E-2 3.551E-1 3.462E-1 6.011E-2 1.010E-1
tauD_2 [ms] 4.297E+1 2.211E+1 9.745E+1 1.186E+2 1.021E+2 8.178E+1 2.344E+1 2.631E+1 8.530E+1 1.702E+2 6.079E+1 4.542E+1
D_2 [mum^2/s] 2.913E-1 1.064E-1 4.966E-1 7.251E-1 1.705E-1 9.645E-2 7.586E-1 4.659E-1 8.425E-1 1.056E+0 2.723E-1 1.410E-1
alpha_2 1.223E+0 2.253E-1 1.254E+0 3.219E-1 1.161E+0 2.326E-1 1.164E+0 1.795E-1 1.235E+0 3.444E-1 1.279E+0 3.791E-1
tauD_m [ms] 7.571E+0 7.191E+0 4.970E+1 5.746E+1 8.200E+0 2.267E+1 4.054E+0 6.697E+0 2.209E+1 4.925E+1 7.884E+0 1.724E+1
D_m [mum^2/s] 2.803E+0 1.861E+0 3.404E+0 1.354E+0 5.665E+0 6.959E+0 1.853E+1 1.447E+1 5.032E+0 1.645E+0 1.181E+1 1.610E+1
R [1/s] 1.208E+1 1.220E+1 2.146E+1 3.932E+1
CPM [kHz] 2.618E+0 1.264E+0 4.544E-1 3.533E-1 9.888E-1 6.897E-1 3.116E-1 2.696E-1
CPM_corr [kHz] 2.660E+0 1.269E+0 5.142E-1 3.979E-1 5.074E-1 1.235E+1 5.987E-1 5.425E-1
Int [kHz] 1.598E+2 1.233E+2 2.362E+2 7.158E+1 1.527E+1 2.593E+1 5.754E+1 6.102E+1
Int_corr [kHz] 1.878E+2 1.404E+2 3.774E+2 1.245E+2 1.808E+1 3.501E+1 9.102E+1 1.067E+2
# samples 384 384 307 307 287 287 255 255 32 32 25 25
# cells 141

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 469.624 - sd = 339.328Int_corr [kHz] - Ch1 - Nuc : ave = 187.795 - sd = 140.422r = 0.711 - r^2 = 0.506
f_1 - Ch1 - Nuc : ave = 0.931 - sd = 0.104D_1 [mum^2/s] - Ch1 - Nuc : ave = 3.095 - sd = 2.306r = -0.550 - r^2 = 0.302
f_2 - Ch1 - Nuc : ave = 0.069 - sd = 0.104D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.291 - sd = 0.106r = -0.036 - r^2 = 0.001
CPM_corr [kHz] - Ch1 - Nuc : ave = 2.660 - sd = 1.269D_m [mum^2/s] - Ch1 - Nuc : ave = 2.803 - sd = 1.861r = -0.004 - r^2 = 1.993E-5
c_corr [nM] - Ch1 - Nuc : ave = 469.624 - sd = 339.328ratioG_corr - Cross - Nuc : ave = 0.004 - sd = 0.041r = -0.035 - r^2 = 0.001
c_corr [nM] - Ch1 - Cyto : ave = 128.364 - sd = 296.746Int_corr [kHz] - Ch1 - Cyto : ave = 18.075 - sd = 35.008r = 0.670 - r^2 = 0.448
f_1 - Ch1 - Cyto : ave = 0.880 - sd = 0.073D_1 [mum^2/s] - Ch1 - Cyto : ave = 21.053 - sd = 16.703r = -0.080 - r^2 = 0.006
f_2 - Ch1 - Cyto : ave = 0.120 - sd = 0.073D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.759 - sd = 0.466r = -0.021 - r^2 = 0.000
CPM_corr [kHz] - Ch1 - Cyto : ave = 0.507 - sd = 12.353D_m [mum^2/s] - Ch1 - Cyto : ave = 18.534 - sd = 14.470r = -0.043 - r^2 = 0.002