Automated F(C)CS analysis of clone # 1238

Created on 12/08/2014
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Gene and protein information

Clone # 1238
Gene symbol: KIF22
Description: kinesin family member 22
Molecular weight (with FP): 100.3 kDa
Localisation: granular nucleus, ave. nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
1238_img_ch1.png1238_img_ch2.png


Point 1, intensity traces and correlation functions
1238_itr_1.png1238_cor_1.png

Point 2, intensity traces and correlation functions
1238_itr_2.png1238_cor_2.png

Point 3, intensity traces and correlation functions
1238_itr_3.png1238_cor_3.png

Point 4, intensity traces and correlation functions
1238_itr_4.png1238_cor_4.png

Point 5, intensity traces and correlation functions
1238_itr_5.png1238_cor_5.png

Point 6, intensity traces and correlation functions
1238_itr_6.png1238_cor_6.png

Sessions

1 20111104\U02--V00--1238
2 20111108\U01--V01--1238
3 20111109\U03--V00--1238
4 20111110\U01--V01--1238
5 20111125\U01--V00--1238
6 20111208\U03--V00--1238
7 20120203\U02--V00--1238
8 20120215\U00--V00--1238
9 20120308\U00--V00--1238
10 20120316\U01--V00--1238
11 20120322\U02--V00--1238

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 8.843E+1 7.869E+1 1.487E+3 7.538E+2 6.674E+0 1.139E+1 3.409E+1 8.650E+1 4.448E+2 5.172E+2 2.013E+1 2.931E+1
N_corr 1.087E+2 9.727E+1 2.483E+3 1.419E+3 1.196E+1 2.737E+1 3.562E+1 9.193E+1 7.623E+2 1.114E+3 3.313E+1 5.783E+1
c [nM] 5.500E+2 4.894E+2 6.899E+3 3.496E+3 3.431E+1 5.855E+1 2.120E+2 5.380E+2 2.063E+3 2.399E+3 1.035E+2 1.507E+2
c_corr [nM] 6.761E+2 6.049E+2 1.152E+4 6.582E+3 6.148E+1 1.407E+2 2.216E+2 5.718E+2 3.536E+3 5.165E+3 1.703E+2 2.973E+2
ratioG 6.159E-2 4.532E-2 3.050E-1 2.256E-1
ratioG_corr 3.077E-2 4.358E-2 1.855E-1 2.408E-1
f_1 7.024E-1 1.185E-1 3.959E-1 2.436E-1 8.945E-1 1.500E-1 8.916E-1 8.354E-2 6.916E-1 3.397E-1 9.702E-1 6.337E-2
tauD_1 [ms] 2.495E+0 2.114E+0 1.860E+0 2.220E-16 4.163E+0 8.104E+0 5.692E-1 4.888E-1 1.860E+0 0.000E+0 1.257E+0 9.162E-1
D_1 [mum^2/s] 6.644E+0 4.723E+0 6.926E+0 0.000E+0 1.526E+1 2.493E+1 2.638E+1 1.318E+1 6.926E+0 0.000E+0 2.769E+1 2.701E+1
alpha_1 9.448E-1 1.067E-1 9.200E-1 3.331E-16 8.028E-1 2.414E-1 1.022E+0 1.490E-1 9.200E-1 0.000E+0 1.044E+0 2.021E-1
f_2 2.976E-1 1.185E-1 6.041E-1 2.436E-1 1.055E-1 1.500E-1 1.084E-1 8.354E-2 3.084E-1 3.397E-1 2.985E-2 6.337E-2
tauD_2 [ms] 4.078E+1 2.932E+1 8.732E+1 9.906E+1 1.909E+2 4.668E+2 1.740E+1 1.596E+1 5.211E+1 6.680E+1 4.891E+1 4.397E+1
D_2 [mum^2/s] 3.576E-1 2.079E-1 4.849E-1 6.082E-1 1.190E-1 6.439E-2 9.521E-1 5.281E-1 1.034E+0 1.240E+0 5.976E-1 5.622E-1
alpha_2 1.628E+0 2.821E-1 1.311E+0 3.121E-1 1.234E+0 2.924E-1 1.136E+0 1.364E-1 1.222E+0 2.555E-1 1.177E+0 2.606E-1
tauD_m [ms] 1.300E+1 7.711E+0 5.135E+1 6.860E+1 2.965E+1 7.161E+1 2.926E+0 4.253E+0 1.628E+1 2.975E+1 4.441E+0 1.009E+1
D_m [mum^2/s] 4.654E+0 3.482E+0 3.076E+0 1.496E+0 1.340E+1 2.184E+1 2.378E+1 1.223E+1 5.249E+0 1.986E+0 2.687E+1 2.614E+1
R [1/s] 1.373E+1 2.540E+1 5.610E+1 5.479E+1
CPM [kHz] 1.012E+0 3.674E-1 3.644E-1 3.610E-1 6.730E-1 4.228E-1 3.172E-1 2.788E-1
CPM_corr [kHz] 1.042E+0 3.758E-1 3.893E-1 3.862E-1 8.751E-1 4.955E-1 1.048E+0 2.504E+0
Int [kHz] 8.073E+1 4.046E+1 2.314E+2 7.552E+1 1.338E+1 1.489E+1 7.836E+1 9.013E+1
Int_corr [kHz] 1.014E+2 5.810E+1 4.048E+2 1.515E+2 1.539E+1 1.598E+1 1.379E+2 1.860E+2
# samples 240 240 166 166 116 116 183 183 16 16 12 12
# cells 174

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 676.133 - sd = 604.943Int_corr [kHz] - Ch1 - Nuc : ave = 101.412 - sd = 58.100r = 0.856 - r^2 = 0.733
f_1 - Ch1 - Nuc : ave = 0.702 - sd = 0.119D_1 [mum^2/s] - Ch1 - Nuc : ave = 6.644 - sd = 4.723r = -0.303 - r^2 = 0.092
f_2 - Ch1 - Nuc : ave = 0.298 - sd = 0.119D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.358 - sd = 0.208r = 0.356 - r^2 = 0.127
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.042 - sd = 0.376D_m [mum^2/s] - Ch1 - Nuc : ave = 4.654 - sd = 3.482r = 0.198 - r^2 = 0.039
c_corr [nM] - Ch1 - Nuc : ave = 676.133 - sd = 604.943ratioG_corr - Cross - Nuc : ave = 0.031 - sd = 0.044r = 0.581 - r^2 = 0.337
c_corr [nM] - Ch1 - Cyto : ave = 221.553 - sd = 571.752Int_corr [kHz] - Ch1 - Cyto : ave = 15.387 - sd = 15.977r = 0.592 - r^2 = 0.350
f_1 - Ch1 - Cyto : ave = 0.892 - sd = 0.084D_1 [mum^2/s] - Ch1 - Cyto : ave = 26.377 - sd = 13.181r = 0.319 - r^2 = 0.102
f_2 - Ch1 - Cyto : ave = 0.108 - sd = 0.084D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.952 - sd = 0.528r = -0.039 - r^2 = 0.002
CPM_corr [kHz] - Ch1 - Cyto : ave = 0.875 - sd = 0.496D_m [mum^2/s] - Ch1 - Cyto : ave = 23.782 - sd = 12.233r = 0.397 - r^2 = 0.158