Automated F(C)CS analysis of clone # ZNF339D10

Created on 12/08/2014
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Gene and protein information

Clone # ZNF339D10
Gene symbol: OVOL2
Description: ovo-like 2 (Drosophila)
Molecular weight (with FP): 57.4 kDa
Localisation: granular nucleus, bright nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
ZNF339D10_img_ch1.pngZNF339D10_img_ch2.png


Point 1, intensity traces and correlation functions
ZNF339D10_itr_1.pngZNF339D10_cor_1.png

Point 2, intensity traces and correlation functions
ZNF339D10_itr_2.pngZNF339D10_cor_2.png

Point 3, intensity traces and correlation functions
ZNF339D10_itr_3.pngZNF339D10_cor_3.png

Point 4, intensity traces and correlation functions
ZNF339D10_itr_4.pngZNF339D10_cor_4.png

Point 5, intensity traces and correlation functions
ZNF339D10_itr_5.pngZNF339D10_cor_5.png

Point 6, intensity traces and correlation functions
ZNF339D10_itr_6.pngZNF339D10_cor_6.png

Sessions

1 20120202\U01--V00--ZNF339D10
2 20120207\U01--V00--ZNF339D10
3 20120221\U01--V00--ZNF339D10
4 20120306\U01--V00--ZNF339D10
5 20120315\U00--V00--ZNF339D10

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.467E+2 7.466E+1 1.093E+3 5.342E+2 2.075E+1 1.880E+1 2.734E+1 3.998E+1 3.098E+2 1.591E+2 3.862E+1 3.906E+1
N_corr 1.545E+2 7.879E+1 1.583E+3 9.445E+2 2.260E+1 1.986E+1 2.551E+1 4.212E+1 3.783E+2 2.282E+2 4.191E+1 4.414E+1
c [nM] 9.121E+2 4.643E+2 5.071E+3 2.478E+3 1.067E+2 9.667E+1 1.701E+2 2.487E+2 1.437E+3 7.379E+2 1.985E+2 2.008E+2
c_corr [nM] 9.609E+2 4.900E+2 7.343E+3 4.381E+3 1.162E+2 1.021E+2 1.586E+2 2.619E+2 1.755E+3 1.058E+3 2.155E+2 2.269E+2
ratioG 1.381E-1 7.633E-2 3.205E-1 1.605E-1
ratioG_corr 2.816E-2 3.934E-2 2.784E-1 1.652E-1
f_1 8.478E-1 1.142E-1 3.649E-1 1.974E-1 9.654E-1 7.154E-2 9.153E-1 7.774E-2 6.139E-1 3.487E-1 9.518E-1 1.186E-1
tauD_1 [ms] 2.901E+0 1.997E+0 1.860E+0 4.441E-16 2.316E+0 2.140E+0 1.086E+0 7.284E-1 1.860E+0 4.441E-16 2.514E+0 6.629E-1
D_1 [mum^2/s] 4.622E+0 1.835E+0 6.926E+0 8.882E-16 8.174E+0 7.545E+0 1.400E+1 8.550E+0 6.926E+0 0.000E+0 5.245E+0 1.981E+0
alpha_1 9.659E-1 9.477E-2 9.200E-1 0.000E+0 7.691E-1 2.013E-1 9.315E-1 1.329E-1 9.200E-1 0.000E+0 1.170E+0 9.632E-2
f_2 1.522E-1 1.142E-1 6.351E-1 1.974E-1 3.464E-2 7.154E-2 8.471E-2 7.774E-2 3.861E-1 3.487E-1 4.818E-2 1.186E-1
tauD_2 [ms] 3.236E+1 1.823E+1 7.347E+1 8.208E+1 8.920E+1 8.513E+1 2.172E+1 1.964E+1 5.311E+1 9.325E+1 5.685E+1 1.880E+1
D_2 [mum^2/s] 3.865E-1 1.321E-1 5.639E-1 8.167E-1 1.981E-1 9.594E-2 6.596E-1 3.000E-1 1.961E+0 1.824E+0 2.305E-1 6.188E-2
alpha_2 1.406E+0 3.123E-1 1.269E+0 3.066E-1 1.141E+0 1.850E-1 1.187E+0 1.732E-1 1.417E+0 4.691E-1 1.094E+0 1.761E-1
tauD_m [ms] 7.104E+0 4.626E+0 4.648E+1 5.493E+1 6.632E+0 1.916E+1 2.662E+0 2.124E+0 1.713E+1 3.340E+1 6.720E+0 1.098E+1
D_m [mum^2/s] 3.948E+0 1.453E+0 2.917E+0 1.272E+0 7.884E+0 7.341E+0 1.269E+1 7.526E+0 5.396E+0 1.319E+0 4.963E+0 1.996E+0
R [1/s] 1.673E+1 1.623E+1 6.293E+0 1.344E+1
CPM [kHz] 1.487E+0 3.019E-1 3.254E-1 2.809E-1 5.830E-1 3.968E-1 2.767E-1 1.361E-1
CPM_corr [kHz] 1.503E+0 3.028E-1 3.981E-1 3.444E-1 8.906E-1 5.984E-1 3.121E-1 1.461E-1
Int [kHz] 2.327E+2 1.144E+2 1.825E+2 6.674E+1 1.117E+1 1.449E+1 5.944E+1 2.181E+1
Int_corr [kHz] 2.464E+2 1.185E+2 3.101E+2 1.284E+2 1.238E+1 1.605E+1 8.336E+1 4.064E+1
# samples 151 151 110 110 106 106 122 122 12 12 11 11
# cells 53

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 960.857 - sd = 490.007Int_corr [kHz] - Ch1 - Nuc : ave = 246.435 - sd = 118.480r = 0.888 - r^2 = 0.788
f_1 - Ch1 - Nuc : ave = 0.848 - sd = 0.114D_1 [mum^2/s] - Ch1 - Nuc : ave = 4.622 - sd = 1.835r = 0.072 - r^2 = 0.005
f_2 - Ch1 - Nuc : ave = 0.152 - sd = 0.114D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.387 - sd = 0.132r = 0.035 - r^2 = 0.001
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.503 - sd = 0.303D_m [mum^2/s] - Ch1 - Nuc : ave = 3.948 - sd = 1.453r = 0.293 - r^2 = 0.086
c_corr [nM] - Ch1 - Nuc : ave = 960.857 - sd = 490.007ratioG_corr - Cross - Nuc : ave = 0.028 - sd = 0.039r = 0.210 - r^2 = 0.044
c_corr [nM] - Ch1 - Cyto : ave = 158.634 - sd = 261.946Int_corr [kHz] - Ch1 - Cyto : ave = 12.383 - sd = 16.047r = 0.528 - r^2 = 0.278
f_1 - Ch1 - Cyto : ave = 0.915 - sd = 0.078D_1 [mum^2/s] - Ch1 - Cyto : ave = 13.998 - sd = 8.550r = -0.091 - r^2 = 0.008
f_2 - Ch1 - Cyto : ave = 0.085 - sd = 0.078D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.660 - sd = 0.300r = -0.089 - r^2 = 0.008
CPM_corr [kHz] - Ch1 - Cyto : ave = 0.891 - sd = 0.598D_m [mum^2/s] - Ch1 - Cyto : ave = 12.687 - sd = 7.526r = 0.397 - r^2 = 0.158