Automated F(C)CS analysis of clone # 2256

Created on 12/08/2014
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Gene and protein information

Clone # 2256
Gene symbol: HOXA9
Description: homeobox A9
Molecular weight (with FP): 57.2 kDa
Localisation: granular nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
2256_img_ch1.png2256_img_ch2.png


Point 1, intensity traces and correlation functions
2256_itr_1.png2256_cor_1.png

Point 2, intensity traces and correlation functions
2256_itr_2.png2256_cor_2.png

Point 3, intensity traces and correlation functions
2256_itr_3.png2256_cor_3.png

Point 4, intensity traces and correlation functions
2256_itr_4.png2256_cor_4.png

Point 5, intensity traces and correlation functions
2256_itr_5.png2256_cor_5.png

Point 6, intensity traces and correlation functions
2256_itr_6.png2256_cor_6.png

Sessions

1 20110915\U03--V01--2256
2 20110916\U03--V01--2256
3 20110922\U00--V01--2256
4 20110930\U02--V01--2256
5 20111011\U00--V01--2256

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.192E+2 9.285E+1 8.667E+2 4.005E+2 2.297E+1 3.017E+1 2.641E+1 3.535E+1 3.647E+2 1.416E+2 2.759E+1 2.823E+1
N_corr 1.368E+2 1.113E+2 1.151E+3 6.680E+2 3.046E+1 4.316E+1 3.244E+1 6.417E+1 5.694E+2 3.497E+2 3.047E+1 2.997E+1
c [nM] 7.413E+2 5.775E+2 4.020E+3 1.857E+3 1.181E+2 1.551E+2 1.642E+2 2.199E+2 1.692E+3 6.566E+2 1.418E+2 1.451E+2
c_corr [nM] 8.507E+2 6.921E+2 5.340E+3 3.098E+3 1.566E+2 2.219E+2 2.017E+2 3.991E+2 2.641E+3 1.622E+3 1.567E+2 1.541E+2
ratioG 1.260E-1 9.941E-2 2.552E-1 1.995E-1
ratioG_corr 3.481E-2 6.591E-2 2.179E-1 2.240E-1
f_1 7.644E-1 1.406E-1 4.644E-1 1.969E-1 8.999E-1 1.330E-1 9.002E-1 8.222E-2 6.000E-1 3.294E-1 9.297E-1 1.003E-1
tauD_1 [ms] 3.353E+0 2.047E+0 1.809E+0 2.181E-2 2.862E+0 2.046E+0 1.174E+0 8.001E-1 1.814E+0 2.528E-2 2.265E+0 1.096E+0
D_1 [mum^2/s] 4.399E+0 3.127E+0 7.121E+0 8.391E-2 6.381E+0 5.391E+0 1.246E+1 6.979E+0 7.104E+0 9.727E-2 6.653E+0 3.383E+0
alpha_1 9.396E-1 1.085E-1 9.200E-1 1.110E-16 7.681E-1 2.132E-1 9.367E-1 1.240E-1 9.200E-1 0.000E+0 9.999E-1 2.127E-1
f_2 2.356E-1 1.406E-1 5.356E-1 1.969E-1 1.001E-1 1.330E-1 9.981E-2 8.222E-2 4.000E-1 3.294E-1 7.027E-2 1.003E-1
tauD_2 [ms] 4.578E+1 3.454E+1 1.130E+2 1.103E+2 1.215E+2 9.721E+1 2.458E+1 1.569E+1 7.676E+1 9.001E+1 7.015E+1 3.140E+1
D_2 [mum^2/s] 3.174E-1 1.470E-1 4.947E-1 7.841E-1 1.461E-1 8.079E-2 5.559E-1 2.385E-1 1.106E+0 1.465E+0 2.162E-1 1.086E-1
alpha_2 1.500E+0 3.472E-1 1.254E+0 3.149E-1 1.256E+0 3.005E-1 1.107E+0 2.178E-1 1.291E+0 4.015E-1 1.274E+0 3.544E-1
tauD_m [ms] 1.174E+1 7.280E+0 6.026E+1 6.385E+1 1.875E+1 3.410E+1 3.760E+0 4.505E+0 2.710E+1 4.214E+1 8.479E+0 9.232E+0
D_m [mum^2/s] 3.195E+0 1.744E+0 3.616E+0 1.191E+0 5.626E+0 4.529E+0 1.116E+1 5.845E+0 5.064E+0 1.438E+0 6.257E+0 3.475E+0
R [1/s] 1.293E+1 1.379E+1 7.615E+0 1.065E+1
CPM [kHz] 1.458E+0 4.375E-1 3.608E-1 2.808E-1 6.123E-1 4.159E-1 2.706E-1 1.211E-1
CPM_corr [kHz] 1.489E+0 4.403E-1 4.380E-1 3.454E-1 9.482E-1 5.918E-1 3.080E-1 1.368E-1
Int [kHz] 1.724E+2 1.245E+2 1.553E+2 4.654E+1 1.384E+1 2.300E+1 8.153E+1 2.809E+1
Int_corr [kHz] 1.995E+2 1.506E+2 2.373E+2 8.471E+1 2.097E+1 5.470E+1 1.472E+2 9.669E+1
# samples 118 118 83 83 71 71 114 114 13 13 11 11
# cells 57

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 850.671 - sd = 692.144Int_corr [kHz] - Ch1 - Nuc : ave = 199.471 - sd = 150.601r = 0.940 - r^2 = 0.883
f_1 - Ch1 - Nuc : ave = 0.764 - sd = 0.141D_1 [mum^2/s] - Ch1 - Nuc : ave = 4.399 - sd = 3.127r = -0.531 - r^2 = 0.282
f_2 - Ch1 - Nuc : ave = 0.236 - sd = 0.141D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.317 - sd = 0.147r = 0.334 - r^2 = 0.112
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.489 - sd = 0.440D_m [mum^2/s] - Ch1 - Nuc : ave = 3.195 - sd = 1.744r = -0.004 - r^2 = 1.917E-5
c_corr [nM] - Ch1 - Nuc : ave = 850.671 - sd = 692.144ratioG_corr - Cross - Nuc : ave = 0.035 - sd = 0.066r = 0.533 - r^2 = 0.284
c_corr [nM] - Ch1 - Cyto : ave = 201.727 - sd = 399.073Int_corr [kHz] - Ch1 - Cyto : ave = 20.967 - sd = 54.703r = 0.674 - r^2 = 0.455
f_1 - Ch1 - Cyto : ave = 0.900 - sd = 0.082D_1 [mum^2/s] - Ch1 - Cyto : ave = 12.460 - sd = 6.979r = -0.078 - r^2 = 0.006
f_2 - Ch1 - Cyto : ave = 0.100 - sd = 0.082D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.556 - sd = 0.239r = 0.188 - r^2 = 0.035
CPM_corr [kHz] - Ch1 - Cyto : ave = 0.948 - sd = 0.592D_m [mum^2/s] - Ch1 - Cyto : ave = 11.155 - sd = 5.845r = 0.010 - r^2 = 0.000