Automated F(C)CS analysis of clone # 2176

Created on 12/08/2014
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Gene and protein information

Clone # 2176
Gene symbol: PMF1
Description: polyamine-modulated factor 1
Molecular weight (with FP): 50.3 kDa
Localisation: homogeneous nucleus, bright nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
2176_img_ch1.png2176_img_ch2.png


Point 1, intensity traces and correlation functions
2176_itr_1.png2176_cor_1.png

Point 2, intensity traces and correlation functions
2176_itr_2.png2176_cor_2.png

Point 3, intensity traces and correlation functions
2176_itr_3.png2176_cor_3.png

Point 4, intensity traces and correlation functions
2176_itr_4.png2176_cor_4.png

Point 5, intensity traces and correlation functions
2176_itr_5.png2176_cor_5.png

Point 6, intensity traces and correlation functions
2176_itr_6.png2176_cor_6.png

Sessions

1 20110914\U03--V00--2176
2 20110920\U03--V00--2176
3 20110922\U02--V01--2176
4 20111123\U01--V01--2176
5 20120116\U02--V00--2176

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.461E+2 8.998E+1 1.032E+3 5.200E+2 2.040E+1 2.400E+1 2.023E+1 1.902E+1 2.739E+2 2.638E+2 1.331E+1 1.890E+1
N_corr 1.682E+2 1.142E+2 1.358E+3 7.936E+2 2.780E+1 3.895E+1 2.192E+1 2.177E+1 3.613E+2 4.536E+2 1.573E+1 2.173E+1
c [nM] 9.084E+2 5.596E+2 4.786E+3 2.412E+3 1.049E+2 1.234E+2 1.258E+2 1.183E+2 1.270E+3 1.223E+3 6.841E+1 9.717E+1
c_corr [nM] 1.046E+3 7.100E+2 6.299E+3 3.681E+3 1.429E+2 2.002E+2 1.363E+2 1.354E+2 1.676E+3 2.104E+3 8.085E+1 1.117E+2
ratioG 1.301E-1 8.594E-2 2.982E-1 2.094E-1
ratioG_corr 2.204E-2 6.519E-2 1.125E-1 9.090E-2
f_1 7.199E-1 1.691E-1 4.537E-1 2.028E-1 9.494E-1 1.083E-1 8.661E-1 6.208E-2 8.328E-1 2.950E-1 9.696E-1 4.531E-2
tauD_1 [ms] 2.560E+0 3.478E+0 1.839E+0 2.849E-2 2.071E+0 5.336E+0 6.972E-1 5.950E-1 1.776E+0 7.994E-1 1.211E+0 1.143E+0
D_1 [mum^2/s] 6.549E+0 4.817E+0 7.005E+0 1.096E-1 2.442E+1 3.128E+1 2.197E+1 1.128E+1 1.790E+1 3.222E+1 3.715E+1 3.691E+1
alpha_1 9.805E-1 1.150E-1 9.203E-1 7.543E-4 7.372E-1 2.215E-1 9.955E-1 1.214E-1 1.163E+0 7.970E-1 9.507E-1 2.190E-1
f_2 2.801E-1 1.691E-1 5.463E-1 2.028E-1 5.056E-2 1.083E-1 1.339E-1 6.208E-2 1.672E-1 2.950E-1 3.043E-2 4.531E-2
tauD_2 [ms] 3.618E+1 3.461E+1 8.066E+1 9.920E+1 9.435E+1 1.048E+2 1.782E+1 1.308E+1 4.123E+1 5.708E+1 3.417E+1 2.356E+1
D_2 [mum^2/s] 3.782E-1 1.410E-1 4.962E-1 6.045E-1 1.987E-1 1.390E-1 8.444E-1 4.455E-1 1.359E+0 1.083E+0 6.435E-1 4.783E-1
alpha_2 1.547E+0 3.270E-1 1.259E+0 3.379E-1 1.192E+0 2.679E-1 1.132E+0 2.143E-1 1.152E+0 2.401E-1 1.099E+0 2.114E-1
tauD_m [ms] 1.168E+1 9.090E+0 4.612E+1 6.605E+1 8.865E+0 3.229E+1 3.386E+0 3.419E+0 1.131E+1 2.155E+1 2.370E+0 2.299E+0
D_m [mum^2/s] 4.501E+0 2.456E+0 3.499E+0 1.216E+0 2.253E+1 2.828E+1 1.941E+1 1.058E+1 1.694E+1 3.258E+1 3.617E+1 3.624E+1
R [1/s] 1.741E+1 1.717E+1 2.252E+1 3.395E+1
CPM [kHz] 1.623E+0 7.627E-1 3.385E-1 3.525E-1 1.117E+0 4.692E-1 2.793E-1 1.971E-1
CPM_corr [kHz] 1.641E+0 7.671E-1 4.154E-1 4.347E-1 1.290E+0 5.070E-1 4.430E-1 2.089E-1
Int [kHz] 2.335E+2 1.314E+2 1.777E+2 5.808E+1 2.332E+1 2.769E+1 6.317E+1 5.614E+1
Int_corr [kHz] 2.687E+2 1.624E+2 2.792E+2 1.054E+2 2.746E+1 3.190E+1 9.779E+1 9.835E+1
# samples 125 125 99 99 89 89 123 123 19 19 16 16
# cells 53

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 1046.170 - sd = 709.968Int_corr [kHz] - Ch1 - Nuc : ave = 268.661 - sd = 162.373r = 0.766 - r^2 = 0.587
f_1 - Ch1 - Nuc : ave = 0.720 - sd = 0.169D_1 [mum^2/s] - Ch1 - Nuc : ave = 6.549 - sd = 4.817r = -0.356 - r^2 = 0.127
f_2 - Ch1 - Nuc : ave = 0.280 - sd = 0.169D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.378 - sd = 0.141r = -0.011 - r^2 = 0.000
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.641 - sd = 0.767D_m [mum^2/s] - Ch1 - Nuc : ave = 4.501 - sd = 2.456r = 0.052 - r^2 = 0.003
c_corr [nM] - Ch1 - Nuc : ave = 1046.170 - sd = 709.968ratioG_corr - Cross - Nuc : ave = 0.022 - sd = 0.065r = 0.323 - r^2 = 0.104
c_corr [nM] - Ch1 - Cyto : ave = 136.346 - sd = 135.366Int_corr [kHz] - Ch1 - Cyto : ave = 27.460 - sd = 31.903r = 0.771 - r^2 = 0.594
f_1 - Ch1 - Cyto : ave = 0.866 - sd = 0.062D_1 [mum^2/s] - Ch1 - Cyto : ave = 21.974 - sd = 11.280r = 0.369 - r^2 = 0.136
f_2 - Ch1 - Cyto : ave = 0.134 - sd = 0.062D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.844 - sd = 0.445r = -0.497 - r^2 = 0.247
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.290 - sd = 0.507D_m [mum^2/s] - Ch1 - Cyto : ave = 19.406 - sd = 10.580r = 0.159 - r^2 = 0.025