Automated F(C)CS analysis of clone # 1988

Created on 12/08/2014
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Gene and protein information

Clone # 1988
Gene symbol: PDZD4
Description: PDZ domain containing 4
Molecular weight (with FP): 113.2 kDa
Localisation: homogeneous nucleus, bright nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
1988_img_ch1.png1988_img_ch2.png


Point 1, intensity traces and correlation functions
1988_itr_1.png1988_cor_1.png

Point 2, intensity traces and correlation functions
1988_itr_2.png1988_cor_2.png

Point 3, intensity traces and correlation functions
1988_itr_3.png1988_cor_3.png

Point 4, intensity traces and correlation functions
1988_itr_4.png1988_cor_4.png

Point 5, intensity traces and correlation functions
1988_itr_5.png1988_cor_5.png

Point 6, intensity traces and correlation functions
1988_itr_6.png1988_cor_6.png

Sessions

1 20111116\U02--V01--1988
2 20111130\U03--V00--1988
3 20111201\U03--V00--1988
4 20111215\U02--V00--1988
5 20120215\U01--V00--1988
6 20120309\U02--V00--1988
7 20120316\U03--V00--1988

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.719E+2 1.200E+2 1.060E+3 5.089E+2 2.337E+1 2.019E+1 1.539E+1 1.876E+1 2.669E+2 2.576E+2 3.192E+0 4.531E-1
N_corr 1.845E+2 1.341E+2 1.378E+3 7.331E+2 2.747E+1 2.724E+1 1.337E+1 1.806E+1 3.912E+2 4.578E+2 3.215E+0 3.911E-1
c [nM] 1.069E+3 7.465E+2 4.917E+3 2.361E+3 1.202E+2 1.038E+2 9.574E+1 1.167E+2 1.238E+3 1.195E+3 1.641E+1 2.329E+0
c_corr [nM] 1.147E+3 8.340E+2 6.390E+3 3.400E+3 1.412E+2 1.401E+2 8.315E+1 1.123E+2 1.814E+3 2.123E+3 1.653E+1 2.011E+0
ratioG 1.461E-1 8.447E-2 2.368E-1 1.214E-1
ratioG_corr 2.165E-2 7.737E-2 7.988E-2 3.838E-2
f_1 8.484E-1 1.731E-1 4.335E-1 2.318E-1 9.826E-1 6.064E-2 9.126E-1 6.429E-2 6.099E-1 3.412E-1 7.975E-1 2.864E-1
tauD_1 [ms] 2.324E+0 1.506E+0 1.860E+0 2.220E-16 1.678E+0 2.075E+0 5.575E-1 4.190E-1 1.860E+0 2.220E-16 1.613E+0 1.223E+0
D_1 [mum^2/s] 5.849E+0 3.698E+0 6.926E+0 8.882E-16 1.685E+1 2.334E+1 2.491E+1 1.142E+1 6.926E+0 8.882E-16 2.539E+1 2.755E+1
alpha_1 1.014E+0 8.713E-2 9.200E-1 0.000E+0 9.200E-1 2.076E-1 9.914E-1 1.152E-1 9.200E-1 0.000E+0 1.055E+0 2.046E-1
f_2 1.516E-1 1.731E-1 5.665E-1 2.318E-1 1.743E-2 6.064E-2 8.737E-2 6.429E-2 3.901E-1 3.412E-1 2.025E-1 2.864E-1
tauD_2 [ms] 2.461E+1 1.256E+1 7.114E+1 8.043E+1 5.498E+1 3.106E+1 1.286E+1 8.649E+0 1.531E+2 2.551E+2 6.170E+1 5.139E+1
D_2 [mum^2/s] 5.201E-1 2.077E-1 7.702E-1 1.100E+0 2.791E-1 1.385E-1 1.048E+0 4.525E-1 1.049E+0 1.189E+0 5.104E-1 4.662E-1
alpha_2 1.385E+0 3.224E-1 1.264E+0 3.221E-1 1.111E+0 1.817E-1 1.141E+0 1.198E-1 1.321E+0 3.235E-1 1.383E+0 4.365E-1
tauD_m [ms] 6.453E+0 6.368E+0 3.794E+1 5.024E+1 2.836E+0 4.875E+0 1.742E+0 1.808E+0 4.828E+1 7.805E+1 2.768E+1 3.798E+1
D_m [mum^2/s] 4.965E+0 2.536E+0 3.555E+0 1.271E+0 1.667E+1 2.337E+1 2.277E+1 1.025E+1 4.901E+0 1.526E+0 2.464E+1 2.815E+1
R [1/s] 3.167E+1 2.814E+1 1.258E+1 1.271E+1
CPM [kHz] 1.740E+0 4.112E-1 3.913E-1 3.132E-1 7.516E-1 4.261E-1 4.624E-1 2.569E-1
CPM_corr [kHz] 1.754E+0 4.136E-1 4.973E-1 4.044E-1 1.122E+0 6.215E-1 5.700E-1 2.205E-1
Int [kHz] 3.048E+2 1.618E+2 1.930E+2 5.496E+1 9.650E+0 9.768E+0 9.411E+1 7.618E+1
Int_corr [kHz] 3.265E+2 1.768E+2 3.129E+2 9.770E+1 1.046E+1 1.010E+1 1.327E+2 1.090E+2
# samples 167 167 127 127 120 120 140 140 7 7 3 3
# cells 59

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 1147.350 - sd = 833.998Int_corr [kHz] - Ch1 - Nuc : ave = 326.453 - sd = 176.849r = 0.770 - r^2 = 0.593
f_1 - Ch1 - Nuc : ave = 0.848 - sd = 0.173D_1 [mum^2/s] - Ch1 - Nuc : ave = 5.849 - sd = 3.698r = 0.131 - r^2 = 0.017
f_2 - Ch1 - Nuc : ave = 0.152 - sd = 0.173D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.520 - sd = 0.208r = -0.205 - r^2 = 0.042
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.754 - sd = 0.414D_m [mum^2/s] - Ch1 - Nuc : ave = 4.965 - sd = 2.536r = 0.152 - r^2 = 0.023
c_corr [nM] - Ch1 - Nuc : ave = 1147.350 - sd = 833.998ratioG_corr - Cross - Nuc : ave = 0.022 - sd = 0.077r = -0.246 - r^2 = 0.061
c_corr [nM] - Ch1 - Cyto : ave = 83.153 - sd = 112.293Int_corr [kHz] - Ch1 - Cyto : ave = 10.458 - sd = 10.102r = 0.571 - r^2 = 0.326
f_1 - Ch1 - Cyto : ave = 0.913 - sd = 0.064D_1 [mum^2/s] - Ch1 - Cyto : ave = 24.912 - sd = 11.421r = 0.198 - r^2 = 0.039
f_2 - Ch1 - Cyto : ave = 0.087 - sd = 0.064D_2 [mum^2/s] - Ch1 - Cyto : ave = 1.048 - sd = 0.452r = -0.190 - r^2 = 0.036
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.122 - sd = 0.622D_m [mum^2/s] - Ch1 - Cyto : ave = 22.766 - sd = 10.253r = 0.206 - r^2 = 0.042