Automated F(C)CS analysis of clone # 1909hi

Created on 12/08/2014
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Gene and protein information

Clone # 1909hi
Gene symbol: DMAP1
Description: DNA methyltransferase 1 associated protein 1
Molecular weight (with FP): 80.0 kDa
Localisation: granular nucleus, ave. nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
1909hi_img_ch1.png1909hi_img_ch2.png


Point 1, intensity traces and correlation functions
1909hi_itr_1.png1909hi_cor_1.png

Point 2, intensity traces and correlation functions
1909hi_itr_2.png1909hi_cor_2.png

Point 3, intensity traces and correlation functions
1909hi_itr_3.png1909hi_cor_3.png

Point 4, intensity traces and correlation functions
1909hi_itr_4.png1909hi_cor_4.png

Point 5, intensity traces and correlation functions
1909hi_itr_5.png1909hi_cor_5.png

Point 6, intensity traces and correlation functions
1909hi_itr_6.png1909hi_cor_6.png

Sessions

1 20130109\U02--V00--1909hi
2 20130110\U01--V01--1909hi
3 20130118\X05--Y00--1909hi
4 20130118\X06--Y00--1909hi

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.718E+2 1.024E+2 9.963E+2 8.431E+2 3.161E+1 3.133E+1 4.890E+1 4.005E+1 1.385E+2 1.637E+2 2.846E+1 1.907E+1
N_corr 1.889E+2 1.201E+2 1.191E+3 9.684E+2 4.542E+1 6.330E+1 6.263E+1 6.106E+1 1.611E+2 3.057E+2 6.772E+1 1.097E+2
c [nM] 1.069E+3 6.370E+2 4.621E+3 3.910E+3 1.625E+2 1.611E+2 3.041E+2 2.491E+2 6.425E+2 7.594E+2 1.463E+2 9.802E+1
c_corr [nM] 1.175E+3 7.468E+2 5.526E+3 4.492E+3 2.335E+2 3.254E+2 3.895E+2 3.798E+2 7.470E+2 1.418E+3 3.482E+2 5.637E+2
ratioG 1.640E-1 8.562E-2 6.301E-1 2.048E-1
ratioG_corr 5.088E-2 1.030E-1 2.242E-1 1.121E-1
f_1 8.354E-1 1.709E-1 4.132E-1 2.460E-1 9.421E-1 1.134E-1 8.882E-1 1.425E-1 3.540E-1 2.543E-1 7.882E-1 1.819E-1
tauD_1 [ms] 6.034E+0 5.793E+0 1.860E+0 4.441E-16 3.842E+0 3.089E+0 7.559E+0 8.946E+0 1.860E+0 2.220E-16 8.591E+0 8.156E+0
D_1 [mum^2/s] 5.072E+0 9.031E+0 6.926E+0 8.882E-16 6.573E+0 1.002E+1 9.526E+0 1.685E+1 6.926E+0 8.882E-16 3.736E+0 7.799E+0
alpha_1 9.949E-1 1.237E-1 9.200E-1 1.110E-16 9.629E-1 2.163E-1 9.402E-1 1.449E-1 9.200E-1 2.220E-16 9.169E-1 1.544E-1
f_2 1.646E-1 1.709E-1 5.868E-1 2.460E-1 5.791E-2 1.134E-1 1.118E-1 1.425E-1 6.460E-1 2.543E-1 2.118E-1 1.819E-1
tauD_2 [ms] 5.645E+1 3.115E+1 7.163E+1 9.786E+1 1.106E+2 7.505E+1 8.529E+1 1.028E+2 4.410E+1 3.776E+1 9.888E+1 7.553E+1
D_2 [mum^2/s] 2.774E-1 2.778E-1 6.168E-1 8.923E-1 1.439E-1 7.198E-2 3.057E-1 3.481E-1 5.399E-1 4.737E-1 1.689E-1 8.435E-2
alpha_2 1.410E+0 3.229E-1 1.459E+0 3.752E-1 1.190E+0 2.697E-1 1.268E+0 2.470E-1 1.196E+0 2.553E-1 1.547E+0 3.803E-1
tauD_m [ms] 1.485E+1 1.118E+1 3.839E+1 6.408E+1 1.010E+1 1.420E+1 1.833E+1 2.733E+1 2.721E+1 2.374E+1 2.632E+1 1.922E+1
D_m [mum^2/s] 3.784E+0 4.557E+0 3.314E+0 1.446E+0 6.286E+0 1.004E+1 8.770E+0 1.649E+1 2.816E+0 1.622E+0 3.113E+0 7.522E+0
R [1/s] 4.705E+0 8.733E+0 1.426E+0 7.877E+0
CPM [kHz] 1.026E+0 7.286E-1 3.381E-1 2.081E-1 2.820E+0 3.521E+0 4.930E-1 3.849E-1
CPM_corr [kHz] 1.046E+0 7.261E-1 4.034E-1 2.749E-1 2.919E+0 3.523E+0 2.082E+0 1.967E+0
Int [kHz] 1.577E+2 1.096E+2 2.011E+2 1.248E+2 9.570E+1 9.476E+1 4.273E+1 4.326E+1
Int_corr [kHz] 1.762E+2 1.282E+2 2.737E+2 1.431E+2 1.310E+2 1.393E+2 7.983E+1 1.134E+2
# samples 189 189 155 155 86 86 131 131 76 76 68 68
# cells 80

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 1174.563 - sd = 746.806Int_corr [kHz] - Ch1 - Nuc : ave = 176.183 - sd = 128.209r = 0.637 - r^2 = 0.406
f_1 - Ch1 - Nuc : ave = 0.835 - sd = 0.171D_1 [mum^2/s] - Ch1 - Nuc : ave = 5.072 - sd = 9.031r = -0.513 - r^2 = 0.263
f_2 - Ch1 - Nuc : ave = 0.165 - sd = 0.171D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.277 - sd = 0.278r = 0.112 - r^2 = 0.013
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.046 - sd = 0.726D_m [mum^2/s] - Ch1 - Nuc : ave = 3.784 - sd = 4.557r = -0.230 - r^2 = 0.053
c_corr [nM] - Ch1 - Nuc : ave = 1174.563 - sd = 746.806ratioG_corr - Cross - Nuc : ave = 0.051 - sd = 0.103r = 0.113 - r^2 = 0.013
c_corr [nM] - Ch1 - Cyto : ave = 389.490 - sd = 379.775Int_corr [kHz] - Ch1 - Cyto : ave = 130.958 - sd = 139.269r = 0.275 - r^2 = 0.075
f_1 - Ch1 - Cyto : ave = 0.888 - sd = 0.143D_1 [mum^2/s] - Ch1 - Cyto : ave = 9.526 - sd = 16.855r = -0.310 - r^2 = 0.096
f_2 - Ch1 - Cyto : ave = 0.112 - sd = 0.143D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.306 - sd = 0.348r = 0.079 - r^2 = 0.006
CPM_corr [kHz] - Ch1 - Cyto : ave = 2.919 - sd = 3.523D_m [mum^2/s] - Ch1 - Cyto : ave = 8.770 - sd = 16.487r = -0.723 - r^2 = 0.523