Automated F(C)CS analysis of clone # 1153

Created on 12/08/2014
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Gene and protein information

Clone # 1153
Gene symbol: ZC3H13
Description: zinc finger CCCH-type containing 13
Molecular weight (with FP): 223.6 kDa
Localisation: homogeneous nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
1153_img_ch1.png1153_img_ch2.png


Point 1, intensity traces and correlation functions
1153_itr_1.png1153_cor_1.png

Point 2, intensity traces and correlation functions
1153_itr_2.png1153_cor_2.png

Point 3, intensity traces and correlation functions
1153_itr_3.png1153_cor_3.png

Point 4, intensity traces and correlation functions
1153_itr_4.png1153_cor_4.png

Point 5, intensity traces and correlation functions
1153_itr_5.png1153_cor_5.png

Point 6, intensity traces and correlation functions
1153_itr_6.png1153_cor_6.png

Sessions

1 20111130\U00--V01--1153
2 20120201\U02--V00--1153

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 4.345E+1 1.637E+0 1.355E+3 1.794E+2 3.755E+1 4.964E+0
N_corr 3.921E+1 6.642E-1 2.107E+3 3.019E+2 3.991E+1 8.939E+0
c [nM] 2.702E+2 1.018E+1 6.287E+3 8.320E+2 2.335E+2 3.087E+1
c_corr [nM] 2.439E+2 4.131E+0 9.772E+3 1.401E+3 2.482E+2 5.560E+1
ratioG
ratioG_corr
f_1 7.147E-1 5.920E-3 4.353E-1 1.176E-1 9.324E-1 5.004E-2
tauD_1 [ms] 3.902E-1 1.083E-2 1.860E+0 0.000E+0 2.265E-1 2.453E-2
D_1 [mum^2/s] 2.721E+1 7.554E-1 6.926E+0 0.000E+0 4.743E+1 5.540E+0
alpha_1 1.100E+0 0.000E+0 9.200E-1 0.000E+0 1.014E+0 1.220E-1
f_2 2.853E-1 5.920E-3 5.647E-1 1.176E-1 6.757E-2 5.004E-2
tauD_2 [ms] 5.017E+0 6.500E-4 2.640E+1 1.254E+1 5.002E+0 1.757E-3
D_2 [mum^2/s] 2.115E+0 2.740E-4 6.665E-1 3.943E-1 2.121E+0 7.449E-4
alpha_2 1.032E+0 2.685E-2 1.530E+0 3.728E-1 9.101E-1 1.803E-1
tauD_m [ms] 1.710E+0 1.983E-2 1.426E+1 4.584E+0 5.497E-1 2.315E-1
D_m [mum^2/s] 2.005E+1 3.912E-1 3.437E+0 4.965E-1 4.428E+1 4.868E+0
R [1/s]
CPM [kHz] 1.138E+0 2.230E-2 2.683E-1 3.785E-2 1.101E+0 1.679E-1
CPM_corr [kHz] 1.178E+0 2.238E-2 2.763E-1 3.835E-2 1.147E+0 1.764E-1
Int [kHz] 6.056E+1 2.490E-1 3.269E+2 3.605E+1 4.806E+1 3.615E-1
Int_corr [kHz] 5.662E+1 1.374E+0 5.279E+2 9.534E+1 5.252E+1 4.635E+0
# samples 2 2 3 3 0 0 3 3 0 0 0 0
# cells 2

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 243.867 - sd = 4.131Int_corr [kHz] - Ch1 - Nuc : ave = 56.617 - sd = 1.374r = NaN - r^2 = NaN
f_1 - Ch1 - Nuc : ave = 0.715 - sd = 0.006D_1 [mum^2/s] - Ch1 - Nuc : ave = 27.212 - sd = 0.755r = NaN - r^2 = NaN
f_2 - Ch1 - Nuc : ave = 0.285 - sd = 0.006D_2 [mum^2/s] - Ch1 - Nuc : ave = 2.115 - sd = 0.000r = NaN - r^2 = NaN
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.178 - sd = 0.022D_m [mum^2/s] - Ch1 - Nuc : ave = 20.048 - sd = 0.391r = NaN - r^2 = NaN
c_corr [nM] - Ch1 - Nuc : ave = 243.867 - sd = 4.131ratioG_corr - Cross - Nuc : ave = NaN - sd = NaNr = NaN - r^2 = NaN
c_corr [nM] - Ch1 - Cyto : ave = 248.240 - sd = 55.597Int_corr [kHz] - Ch1 - Cyto : ave = 52.520 - sd = 4.635r = NaN - r^2 = NaN
f_1 - Ch1 - Cyto : ave = 0.932 - sd = 0.050D_1 [mum^2/s] - Ch1 - Cyto : ave = 47.432 - sd = 5.540r = NaN - r^2 = NaN
f_2 - Ch1 - Cyto : ave = 0.068 - sd = 0.050D_2 [mum^2/s] - Ch1 - Cyto : ave = 2.121 - sd = 0.001r = NaN - r^2 = NaN
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.147 - sd = 0.176D_m [mum^2/s] - Ch1 - Cyto : ave = 44.284 - sd = 4.868r = NaN - r^2 = NaN