Automated F(C)CS analysis of clone # HP1

Created on 12/08/2014
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Gene and protein information

Clone # HP1
Gene symbol: CBX5
Description: chromobox homolog 5, heterochromatin protein 1 isoform alpha
Molecular weight (with FP): 49.2 kDa
Localisation: granular nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
HP1_img_ch1.pngHP1_img_ch2.png


Point 1, intensity traces and correlation functions
HP1_itr_1.pngHP1_cor_1.png

Point 2, intensity traces and correlation functions
HP1_itr_2.pngHP1_cor_2.png

Point 3, intensity traces and correlation functions
HP1_itr_3.pngHP1_cor_3.png

Point 4, intensity traces and correlation functions
HP1_itr_4.pngHP1_cor_4.png

Point 5, intensity traces and correlation functions
HP1_itr_5.pngHP1_cor_5.png

Point 6, intensity traces and correlation functions
HP1_itr_6.pngHP1_cor_6.png

Sessions

1 20120712\U00--V00--HP1
2 20120712\U00--V01--HP1
3 20120914\U03--V00--HP1
4 20121017\U00--V00--HP1
5 20121023\U00--V00--HP1
6 20121024\U00--V00--HP1

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.021E+2 5.340E+1 1.183E+3 6.579E+2 8.727E+0 1.076E+1 3.306E+1 5.667E+1 5.354E+2 4.214E+2 1.172E+1 2.091E+1
N_corr 1.091E+2 6.354E+1 1.730E+3 1.040E+3 1.033E+1 1.414E+1 3.616E+1 7.609E+1 8.285E+2 7.539E+2 1.788E+1 4.357E+1
c [nM] 6.353E+2 3.321E+2 5.487E+3 3.052E+3 4.487E+1 5.533E+1 2.056E+2 3.525E+2 2.484E+3 1.955E+3 6.026E+1 1.075E+2
c_corr [nM] 6.784E+2 3.952E+2 8.024E+3 4.824E+3 5.311E+1 7.269E+1 2.249E+2 4.732E+2 3.843E+3 3.497E+3 9.191E+1 2.240E+2
ratioG 7.689E-2 6.464E-2 1.620E-1 1.947E-1
ratioG_corr 2.103E-2 6.971E-2 1.081E-1 1.958E-1
f_1 8.146E-1 1.050E-1 4.415E-1 2.411E-1 9.151E-1 1.509E-1 8.799E-1 9.402E-2 5.566E-1 2.903E-1 8.609E-1 1.884E-1
tauD_1 [ms] 2.853E+0 1.158E+0 1.860E+0 1.332E-15 4.258E+0 7.426E+0 1.123E+0 1.397E+0 1.860E+0 2.220E-16 5.588E+0 1.092E+1
D_1 [mum^2/s] 4.351E+0 1.981E+0 6.926E+0 6.217E-15 7.407E+0 1.212E+1 2.289E+1 2.107E+1 6.926E+0 0.000E+0 6.450E+0 6.482E+0
alpha_1 9.443E-1 1.122E-1 9.200E-1 1.221E-15 9.085E-1 2.159E-1 1.014E+0 1.800E-1 9.200E-1 0.000E+0 9.777E-1 1.645E-1
f_2 1.854E-1 1.050E-1 5.585E-1 2.411E-1 8.494E-2 1.509E-1 1.201E-1 9.402E-2 4.434E-1 2.903E-1 1.391E-1 1.884E-1
tauD_2 [ms] 4.625E+1 2.801E+1 1.282E+2 1.393E+2 1.735E+2 2.057E+2 3.221E+1 4.385E+1 1.793E+2 2.026E+2 3.081E+2 7.078E+2
D_2 [mum^2/s] 2.874E-1 1.241E-1 3.679E-1 6.835E-1 1.160E-1 1.398E-1 5.723E-1 3.369E-1 3.082E-1 6.399E-1 1.242E-1 9.989E-2
alpha_2 1.517E+0 2.937E-1 1.285E+0 3.095E-1 1.188E+0 2.549E-1 1.197E+0 2.198E-1 1.253E+0 2.913E-1 1.181E+0 2.921E-1
tauD_m [ms] 1.095E+1 8.815E+0 6.962E+1 8.400E+1 2.776E+1 7.892E+1 5.809E+0 1.452E+1 9.316E+1 1.341E+2 8.246E+1 2.158E+2
D_m [mum^2/s] 3.516E+0 1.440E+0 3.314E+0 1.520E+0 6.614E+0 1.061E+1 2.062E+1 1.990E+1 4.013E+0 1.896E+0 5.589E+0 5.792E+0
R [1/s] 1.616E+1 1.486E+1 1.465E+1 1.310E+1
CPM [kHz] 1.423E+0 4.519E-1 5.474E-1 4.778E-1 5.024E-1 5.679E-1 6.223E-1 5.768E-1
CPM_corr [kHz] 1.448E+0 4.511E-1 6.028E-1 5.259E-1 4.581E-1 7.499E+0 6.717E-1 9.533E-1
Int [kHz] 1.513E+2 9.214E+1 2.607E+2 7.959E+1 2.172E+1 4.658E+1 1.530E+2 9.738E+1
Int_corr [kHz] 1.616E+2 9.749E+1 4.127E+2 1.349E+2 2.790E+1 6.596E+1 2.514E+2 1.783E+2
# samples 839 839 628 628 468 468 398 398 103 103 73 73
# cells 304

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 678.408 - sd = 395.161Int_corr [kHz] - Ch1 - Nuc : ave = 161.558 - sd = 97.492r = 0.809 - r^2 = 0.655
f_1 - Ch1 - Nuc : ave = 0.815 - sd = 0.105D_1 [mum^2/s] - Ch1 - Nuc : ave = 4.351 - sd = 1.981r = -0.483 - r^2 = 0.234
f_2 - Ch1 - Nuc : ave = 0.185 - sd = 0.105D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.287 - sd = 0.124r = -0.034 - r^2 = 0.001
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.448 - sd = 0.451D_m [mum^2/s] - Ch1 - Nuc : ave = 3.516 - sd = 1.440r = 0.122 - r^2 = 0.015
c_corr [nM] - Ch1 - Nuc : ave = 678.408 - sd = 395.161ratioG_corr - Cross - Nuc : ave = 0.021 - sd = 0.070r = 0.087 - r^2 = 0.008
c_corr [nM] - Ch1 - Cyto : ave = 224.896 - sd = 473.192Int_corr [kHz] - Ch1 - Cyto : ave = 27.898 - sd = 65.965r = 0.750 - r^2 = 0.562
f_1 - Ch1 - Cyto : ave = 0.880 - sd = 0.094D_1 [mum^2/s] - Ch1 - Cyto : ave = 22.894 - sd = 21.067r = 0.151 - r^2 = 0.023
f_2 - Ch1 - Cyto : ave = 0.120 - sd = 0.094D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.572 - sd = 0.337r = 0.020 - r^2 = 0.000
CPM_corr [kHz] - Ch1 - Cyto : ave = 0.458 - sd = 7.499D_m [mum^2/s] - Ch1 - Cyto : ave = 20.616 - sd = 19.905r = -0.273 - r^2 = 0.075