Automated F(C)CS analysis of clone # H2Ahi

Created on 12/08/2014
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Gene and protein information

Clone # H2Ahi
Gene symbol: HIST3H2A
Description: histone cluster 3, H2A
Molecular weight (with FP): 41.1 kDa
Localisation: granular nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
H2Ahi_img_ch1.pngH2Ahi_img_ch2.png


Point 1, intensity traces and correlation functions
H2Ahi_itr_1.pngH2Ahi_cor_1.png

Point 2, intensity traces and correlation functions
H2Ahi_itr_2.pngH2Ahi_cor_2.png

Point 3, intensity traces and correlation functions
H2Ahi_itr_3.pngH2Ahi_cor_3.png

Point 4, intensity traces and correlation functions
H2Ahi_itr_4.pngH2Ahi_cor_4.png

Point 5, intensity traces and correlation functions
H2Ahi_itr_5.pngH2Ahi_cor_5.png

Point 6, intensity traces and correlation functions
H2Ahi_itr_6.pngH2Ahi_cor_6.png

Sessions

1 20130109\U03--V00--H2Ahi
2 20130110\U01--V00--H2Ahi

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 3.523E+2 2.039E+2 6.570E+2 2.915E+2 1.329E+2 1.118E+2 3.118E+1 4.102E+1 5.156E+2 3.491E+2 3.232E+1 3.579E+1
N_corr 5.564E+2 3.171E+2 8.795E+2 4.145E+2 4.066E+2 3.736E+2 3.745E+1 8.646E+1 8.958E+2 5.645E+2 1.876E+2 2.177E+2
c [nM] 2.191E+3 1.268E+3 3.048E+3 1.352E+3 6.833E+2 5.748E+2 1.939E+2 2.551E+2 2.391E+3 1.619E+3 1.661E+2 1.840E+2
c_corr [nM] 3.461E+3 1.972E+3 4.080E+3 1.923E+3 2.091E+3 1.921E+3 2.329E+2 5.377E+2 4.155E+3 2.619E+3 9.645E+2 1.119E+3
ratioG 3.379E-1 1.520E-1 1.799E-1 7.139E-2
ratioG_corr 4.424E-1 2.624E-1 3.314E-1 1.723E-1
f_1 7.625E-1 1.473E-1 4.274E-1 2.756E-1 7.728E-1 2.220E-1 9.527E-1 5.321E-2 6.321E-1 3.273E-1 8.987E-1 1.266E-1
tauD_1 [ms] 2.805E+0 3.435E+0 1.860E+0 4.441E-16 3.618E+0 5.031E+0 3.554E-1 3.848E-1 1.860E+0 2.220E-16 2.716E+0 9.729E-1
D_1 [mum^2/s] 7.195E+0 6.004E+0 6.926E+0 8.882E-16 7.056E+0 1.264E+1 4.437E+1 2.208E+1 6.926E+0 8.882E-16 4.904E+0 1.405E+0
alpha_1 9.492E-1 1.632E-1 9.200E-1 0.000E+0 9.145E-1 2.359E-1 1.057E+0 1.527E-1 9.200E-1 1.110E-16 9.504E-1 2.020E-1
f_2 2.375E-1 1.473E-1 5.726E-1 2.756E-1 2.272E-1 2.220E-1 4.726E-2 5.321E-2 3.679E-1 3.273E-1 1.013E-1 1.266E-1
tauD_2 [ms] 5.921E+1 6.541E+1 7.374E+1 8.712E+1 1.235E+2 1.955E+2 1.744E+1 1.951E+1 9.717E+1 7.748E+1 6.771E+1 1.790E+1
D_2 [mum^2/s] 2.841E-1 2.073E-1 7.266E-1 9.530E-1 1.704E-1 9.168E-2 1.079E+0 6.468E-1 3.004E-1 2.913E-1 1.907E-1 5.328E-2
alpha_2 1.528E+0 3.619E-1 1.401E+0 4.317E-1 1.397E+0 4.029E-1 1.121E+0 1.349E-1 1.201E+0 2.899E-1 1.326E+0 3.898E-1
tauD_m [ms] 1.628E+1 1.949E+1 3.644E+1 4.991E+1 3.381E+1 5.252E+1 1.611E+0 3.196E+0 2.649E+1 2.107E+1 1.125E+1 1.253E+1
D_m [mum^2/s] 5.515E+0 5.065E+0 3.516E+0 1.510E+0 5.798E+0 1.240E+1 4.279E+1 2.197E+1 4.524E+0 2.115E+0 4.569E+0 1.639E+0
R [1/s] 5.759E+0 9.541E+0 1.896E+1 2.068E+1
CPM [kHz] 4.880E-1 1.903E-1 3.685E-1 2.618E-1 3.236E-1 1.161E-1 5.035E-1 3.563E-1
CPM_corr [kHz] 4.963E-1 1.913E-1 4.337E-1 2.932E-1 5.014E-1 2.205E-1 5.652E-1 4.193E-1
Int [kHz] 1.509E+2 9.377E+1 1.460E+2 3.297E+1 1.084E+1 1.424E+1 1.020E+2 3.600E+1
Int_corr [kHz] 2.367E+2 1.320E+2 2.339E+2 6.754E+1 1.508E+1 2.975E+1 2.038E+2 9.943E+1
# samples 137 137 118 118 101 101 95 95 9 9 4 4
# cells 74

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 3460.501 - sd = 1971.837Int_corr [kHz] - Ch1 - Nuc : ave = 236.699 - sd = 131.967r = 0.876 - r^2 = 0.768
f_1 - Ch1 - Nuc : ave = 0.762 - sd = 0.147D_1 [mum^2/s] - Ch1 - Nuc : ave = 7.195 - sd = 6.004r = -0.316 - r^2 = 0.100
f_2 - Ch1 - Nuc : ave = 0.238 - sd = 0.147D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.284 - sd = 0.207r = -0.005 - r^2 = 2.197E-5
CPM_corr [kHz] - Ch1 - Nuc : ave = 0.496 - sd = 0.191D_m [mum^2/s] - Ch1 - Nuc : ave = 5.515 - sd = 5.065r = -0.364 - r^2 = 0.133
c_corr [nM] - Ch1 - Nuc : ave = 3460.501 - sd = 1971.837ratioG_corr - Cross - Nuc : ave = 0.442 - sd = 0.262r = 0.630 - r^2 = 0.396
c_corr [nM] - Ch1 - Cyto : ave = 232.881 - sd = 537.732Int_corr [kHz] - Ch1 - Cyto : ave = 15.081 - sd = 29.746r = 0.808 - r^2 = 0.653
f_1 - Ch1 - Cyto : ave = 0.953 - sd = 0.053D_1 [mum^2/s] - Ch1 - Cyto : ave = 44.365 - sd = 22.079r = -0.045 - r^2 = 0.002
f_2 - Ch1 - Cyto : ave = 0.047 - sd = 0.053D_2 [mum^2/s] - Ch1 - Cyto : ave = 1.079 - sd = 0.647r = -0.058 - r^2 = 0.003
CPM_corr [kHz] - Ch1 - Cyto : ave = 0.501 - sd = 0.221D_m [mum^2/s] - Ch1 - Cyto : ave = 42.793 - sd = 21.974r = 0.172 - r^2 = 0.029