Automated F(C)CS analysis of clone # H2A

Created on 12/08/2014
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Gene and protein information

Clone # H2A
Gene symbol: HIST3H2A
Description: histone cluster 3, H2A
Molecular weight (with FP): 41.1 kDa
Localisation: granular nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
H2A_img_ch1.pngH2A_img_ch2.png


Point 1, intensity traces and correlation functions
H2A_itr_1.pngH2A_cor_1.png

Point 2, intensity traces and correlation functions
H2A_itr_2.pngH2A_cor_2.png

Point 3, intensity traces and correlation functions
H2A_itr_3.pngH2A_cor_3.png

Point 4, intensity traces and correlation functions
H2A_itr_4.pngH2A_cor_4.png

Point 5, intensity traces and correlation functions
H2A_itr_5.pngH2A_cor_5.png

Point 6, intensity traces and correlation functions
H2A_itr_6.pngH2A_cor_6.png

Sessions

1 20120322\U00--V00--H2A
2 20120712\U01--V00--H2A
3 20120712\U01--V01--H2A
4 20120914\U01--V00--H2A

Results table

Ch1 ave
Nuc
Ch1 sd
Nuc
Ch2 ave
Nuc
Ch2 sd
Nuc
Cross ave
Nuc
Cross sd
Nuc
Ch1 ave
Cyto
Ch1 sd
Cyto
Ch2 ave
Cyto
Ch2 sd
Cyto
Cross ave
Cyto
Cross sd
Cyto
N 5.932E+1 4.028E+1 1.400E+3 8.299E+2 4.165E+0 3.554E+0 2.277E+1 4.265E+1 3.609E+2 4.737E+2 2.761E+1 7.149E+1
N_corr 9.002E+1 6.529E+1 2.306E+3 1.493E+3 1.037E+1 8.615E+0 2.600E+1 6.915E+1 6.482E+2 1.148E+3 9.611E+1 2.172E+2
c [nM] 3.689E+2 2.505E+2 6.494E+3 3.849E+3 2.141E+1 1.827E+1 1.416E+2 2.653E+2 1.674E+3 2.197E+3 1.419E+2 3.675E+2
c_corr [nM] 5.599E+2 4.060E+2 1.069E+4 6.923E+3 5.329E+1 4.429E+1 1.617E+2 4.301E+2 3.006E+3 5.324E+3 4.941E+2 1.117E+3
ratioG 5.709E-2 3.510E-2 2.496E-1 1.893E-1
ratioG_corr 5.337E-2 4.511E-2 2.638E-1 2.870E-1
f_1 8.559E-1 1.201E-1 4.700E-1 2.751E-1 8.925E-1 1.528E-1 9.341E-1 8.492E-2 6.775E-1 3.737E-1 9.075E-1 2.113E-1
tauD_1 [ms] 1.186E+0 5.156E-1 1.860E+0 4.441E-16 2.743E+0 2.359E+0 7.039E-1 1.435E+0 2.282E+0 2.203E+0 6.179E+0 1.198E+1
D_1 [mum^2/s] 1.015E+1 3.403E+0 6.926E+0 2.665E-15 8.155E+0 9.817E+0 3.439E+1 2.316E+1 6.700E+0 1.096E+0 6.062E+0 4.274E+0
alpha_1 1.040E+0 1.444E-1 9.200E-1 6.661E-16 9.931E-1 2.542E-1 1.042E+0 1.740E-1 9.208E-1 7.047E-2 1.014E+0 2.634E-1
f_2 1.441E-1 1.201E-1 5.300E-1 2.751E-1 1.075E-1 1.528E-1 6.590E-2 8.492E-2 3.225E-1 3.737E-1 9.250E-2 2.113E-1
tauD_2 [ms] 2.662E+1 1.889E+1 1.121E+2 1.407E+2 1.628E+2 1.366E+2 1.706E+1 1.926E+1 1.430E+2 1.717E+2 4.331E+2 1.226E+3
D_2 [mum^2/s] 5.351E-1 2.530E-1 5.793E-1 9.551E-1 1.147E-1 6.327E-2 9.821E-1 5.370E-1 7.575E-1 1.273E+0 1.686E-1 8.910E-2
alpha_2 1.349E+0 2.507E-1 1.372E+0 3.373E-1 1.265E+0 2.531E-1 1.178E+0 1.227E-1 1.321E+0 3.249E-1 1.251E+0 2.852E-1
tauD_m [ms] 5.248E+0 4.987E+0 5.564E+1 7.899E+1 3.117E+1 5.788E+1 2.123E+0 4.818E+0 4.636E+1 1.041E+2 2.640E+1 6.086E+1
D_m [mum^2/s] 8.802E+0 3.172E+0 3.678E+0 1.618E+0 7.049E+0 7.371E+0 3.258E+1 2.301E+1 4.947E+0 2.075E+0 5.213E+0 3.749E+0
R [1/s] 3.279E+1 2.692E+1 3.331E+1 2.874E+1
CPM [kHz] 9.807E-1 3.399E-1 4.907E-1 4.894E-1 4.287E-1 5.048E-1 5.612E-1 5.983E-1
CPM_corr [kHz] 1.020E+0 3.476E-1 5.109E-1 5.071E-1 8.476E-1 1.381E+0 -2.635E-2 4.008E+0
Int [kHz] 5.783E+1 3.285E+1 2.755E+2 9.128E+1 8.417E+0 2.026E+1 1.083E+2 9.725E+1
Int_corr [kHz] 8.798E+1 4.495E+1 4.613E+2 1.592E+2 1.280E+1 3.692E+1 1.875E+2 2.381E+2
# samples 321 321 245 245 116 116 173 173 29 29 15 15
# cells 113

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 559.853 - sd = 406.041Int_corr [kHz] - Ch1 - Nuc : ave = 87.983 - sd = 44.952r = 0.735 - r^2 = 0.540
f_1 - Ch1 - Nuc : ave = 0.856 - sd = 0.120D_1 [mum^2/s] - Ch1 - Nuc : ave = 10.155 - sd = 3.403r = 0.301 - r^2 = 0.091
f_2 - Ch1 - Nuc : ave = 0.144 - sd = 0.120D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.535 - sd = 0.253r = -0.397 - r^2 = 0.157
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.020 - sd = 0.348D_m [mum^2/s] - Ch1 - Nuc : ave = 8.802 - sd = 3.172r = 0.180 - r^2 = 0.032
c_corr [nM] - Ch1 - Nuc : ave = 559.853 - sd = 406.041ratioG_corr - Cross - Nuc : ave = 0.053 - sd = 0.045r = 0.194 - r^2 = 0.037
c_corr [nM] - Ch1 - Cyto : ave = 161.717 - sd = 430.050Int_corr [kHz] - Ch1 - Cyto : ave = 12.799 - sd = 36.921r = 0.693 - r^2 = 0.480
f_1 - Ch1 - Cyto : ave = 0.934 - sd = 0.085D_1 [mum^2/s] - Ch1 - Cyto : ave = 34.391 - sd = 23.156r = -0.107 - r^2 = 0.011
f_2 - Ch1 - Cyto : ave = 0.066 - sd = 0.085D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.982 - sd = 0.537r = -0.067 - r^2 = 0.004
CPM_corr [kHz] - Ch1 - Cyto : ave = 0.848 - sd = 1.381D_m [mum^2/s] - Ch1 - Cyto : ave = 32.582 - sd = 23.013r = 0.068 - r^2 = 0.005