Automated F(C)CS analysis of clone # EYFPN2

Created on 12/08/2014
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Gene and protein information

Clone # EYFPN2
Gene symbol: EYFPN2
Description: enhanced yellow fluorescent protein N2
Molecular weight (with FP): 27.0 kDa
Localisation: homogeneous nucleus, ave. nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFPN2 construct), Ch2 (H2B-mCherry) with measurement points
EYFPN2_img_ch1.pngEYFPN2_img_ch2.png


Point 1, intensity traces and correlation functions
EYFPN2_itr_1.pngEYFPN2_cor_1.png

Point 2, intensity traces and correlation functions
EYFPN2_itr_2.pngEYFPN2_cor_2.png

Point 3, intensity traces and correlation functions
EYFPN2_itr_3.pngEYFPN2_cor_3.png

Point 4, intensity traces and correlation functions
EYFPN2_itr_4.pngEYFPN2_cor_4.png

Point 5, intensity traces and correlation functions
EYFPN2_itr_5.pngEYFPN2_cor_5.png

Point 6, intensity traces and correlation functions
EYFPN2_itr_6.pngEYFPN2_cor_6.png

Sessions

1 20120126\U03--V00--EYFPN2
2 20120127\U03--V00--EYFPN2
3 20120131\U03--V00--EYFPN2
4 20120201\U03--V00--EYFPN2
5 20120202\U03--V00--EYFPN2
6 20120203\U03--V00--EYFPN2
7 20120207\U03--V00--EYFPN2
8 20120214\U03--V00--EYFPN2
9 20120215\U03--V00--EYFPN2
10 20120217\U03--V00--EYFPN2
11 20120221\U03--V00--EYFPN2
12 20120223\U03--V00--EYFPN2
13 20120228\U03--V00--EYFPN2
14 20120229\U03--V00--EYFPN2
15 20120306\U03--V00--EYFPN2
16 20120307\U03--V00--EYFPN2
17 20120308\U03--V00--EYFPN2
18 20120309\U03--V00--EYFPN2
19 20120315\U03--V00--EYFPN2
20 20120316\U02--V00--EYFPN2
21 20120322\U03--V00--EYFPN2

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 6.290E+1 2.876E+1 1.033E+3 5.309E+2 5.467E+0 5.655E+0 5.600E+1 5.573E+1 1.463E+2 1.333E+2 3.010E+1 1.972E+1
N_corr 6.322E+1 2.937E+1 1.573E+3 9.606E+2 5.615E+0 5.872E+0 5.889E+1 5.772E+1 9.789E+1 2.341E+2 3.344E+1 2.198E+1
c [nM] 3.912E+2 1.789E+2 4.791E+3 2.463E+3 2.811E+1 2.907E+1 3.483E+2 3.466E+2 6.786E+2 6.185E+2 1.548E+2 1.014E+2
c_corr [nM] 3.932E+2 1.827E+2 7.295E+3 4.456E+3 2.886E+1 3.019E+1 3.662E+2 3.590E+2 4.541E+2 1.086E+3 1.719E+2 1.130E+2
ratioG 7.288E-2 4.259E-2 5.660E-1 2.105E-1
ratioG_corr 6.209E-3 2.313E-2 9.028E-2 8.767E-2
f_1 9.833E-1 4.680E-2 4.868E-1 2.617E-1 9.945E-1 2.492E-2 9.619E-1 1.014E-1 9.029E-1 2.090E-1 9.607E-1 4.773E-2
tauD_1 [ms] 3.770E-1 7.425E-2 1.860E+0 4.441E-16 2.697E-1 2.438E-1 4.487E-1 2.858E-1 1.860E+0 2.220E-16 2.245E-1 2.213E-1
D_1 [mum^2/s] 2.916E+1 5.848E+0 6.926E+0 5.329E-15 9.091E+1 2.289E+2 2.751E+1 8.475E+0 6.926E+0 8.882E-16 7.489E+1 5.663E+1
alpha_1 1.074E+0 1.003E-1 9.200E-1 1.110E-15 1.094E+0 2.322E-1 1.021E+0 1.247E-1 9.200E-1 3.331E-16 1.016E+0 1.996E-1
f_2 1.667E-2 4.680E-2 5.132E-1 2.617E-1 5.494E-3 2.492E-2 3.808E-2 1.014E-1 9.707E-2 2.090E-1 3.929E-2 4.773E-2
tauD_2 [ms] 9.404E+0 1.318E+1 7.621E+1 9.389E+1 3.252E+1 2.233E+1 1.668E+1 2.801E+1 2.705E+1 5.947E+1 1.264E+1 1.027E+1
D_2 [mum^2/s] 3.102E+0 3.912E+0 7.466E-1 9.940E-1 6.547E-1 1.383E+0 2.180E+0 4.206E+0 2.201E+0 3.898E+0 1.417E+0 1.281E+0
alpha_2 1.055E+0 9.796E-2 1.297E+0 3.351E-1 1.069E+0 6.428E-2 1.030E+0 1.723E-1 1.083E+0 1.358E-1 1.082E+0 4.784E-2
tauD_m [ms] 4.563E-1 2.055E-1 3.705E+1 5.224E+1 3.964E-1 9.184E-1 1.104E+0 2.461E+0 4.338E+0 1.582E+1 5.889E-1 5.995E-1
D_m [mum^2/s] 2.875E+1 5.764E+0 3.867E+0 1.475E+0 9.047E+1 2.288E+2 2.626E+1 7.130E+0 7.015E+0 1.317E+0 7.202E+1 5.643E+1
R [1/s] 3.553E+2 5.720E+2 2.528E+2 6.739E+2
CPM [kHz] 1.662E+0 1.699E-1 3.647E-1 3.263E-1 1.631E+0 5.687E-1 1.784E-1 1.195E-1
CPM_corr [kHz] 1.693E+0 1.724E-1 4.087E-1 3.625E-1 1.674E+0 5.655E-1 7.169E-1 3.736E-1
Int [kHz] 1.255E+2 5.353E+1 1.875E+2 6.896E+1 9.899E+1 5.959E+1 2.954E+1 3.329E+1
Int_corr [kHz] 1.281E+2 5.461E+1 3.138E+2 1.376E+2 1.050E+2 6.173E+1 3.834E+1 5.909E+1
# samples 627 627 482 482 447 447 433 433 189 189 171 171
# cells 236

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 393.181 - sd = 182.678Int_corr [kHz] - Ch1 - Nuc : ave = 128.080 - sd = 54.608r = 0.953 - r^2 = 0.908
f_1 - Ch1 - Nuc : ave = 0.983 - sd = 0.047D_1 [mum^2/s] - Ch1 - Nuc : ave = 29.165 - sd = 5.848r = -0.120 - r^2 = 0.015
f_2 - Ch1 - Nuc : ave = 0.017 - sd = 0.047D_2 [mum^2/s] - Ch1 - Nuc : ave = 3.102 - sd = 3.912r = -0.315 - r^2 = 0.099
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.693 - sd = 0.172D_m [mum^2/s] - Ch1 - Nuc : ave = 28.749 - sd = 5.764r = 0.061 - r^2 = 0.004
c_corr [nM] - Ch1 - Nuc : ave = 393.181 - sd = 182.678ratioG_corr - Cross - Nuc : ave = 0.006 - sd = 0.023r = 0.112 - r^2 = 0.012
c_corr [nM] - Ch1 - Cyto : ave = 366.228 - sd = 359.003Int_corr [kHz] - Ch1 - Cyto : ave = 104.978 - sd = 61.728r = 0.627 - r^2 = 0.394
f_1 - Ch1 - Cyto : ave = 0.962 - sd = 0.101D_1 [mum^2/s] - Ch1 - Cyto : ave = 27.512 - sd = 8.475r = -0.162 - r^2 = 0.026
f_2 - Ch1 - Cyto : ave = 0.038 - sd = 0.101D_2 [mum^2/s] - Ch1 - Cyto : ave = 2.180 - sd = 4.206r = -0.209 - r^2 = 0.044
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.674 - sd = 0.566D_m [mum^2/s] - Ch1 - Cyto : ave = 26.260 - sd = 7.130r = 0.286 - r^2 = 0.082