Automated F(C)CS analysis of clone # EYFPC1hi

Created on 12/08/2014
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Gene and protein information

Clone # EYFPC1hi
Gene symbol: EYFPC1
Description: enhanced yellow fluorescent protein C1
Molecular weight (with FP): 27.0 kDa
Localisation: homogeneous nucleus, ave. nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
EYFPC1hi_img_ch1.pngEYFPC1hi_img_ch2.png


Point 1, intensity traces and correlation functions
EYFPC1hi_itr_1.pngEYFPC1hi_cor_1.png

Point 2, intensity traces and correlation functions
EYFPC1hi_itr_2.pngEYFPC1hi_cor_2.png

Point 3, intensity traces and correlation functions
EYFPC1hi_itr_3.pngEYFPC1hi_cor_3.png

Point 4, intensity traces and correlation functions
EYFPC1hi_itr_4.pngEYFPC1hi_cor_4.png

Point 5, intensity traces and correlation functions
EYFPC1hi_itr_5.pngEYFPC1hi_cor_5.png

Point 6, intensity traces and correlation functions
EYFPC1hi_itr_6.pngEYFPC1hi_cor_6.png

Sessions

1 20130109\U00--V00--EYFPC1hi
2 20130110\U00--V00--EYFPC1hi

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.400E+3 8.668E+2 1.406E+3 6.133E+2 6.054E+2 4.552E+2 1.058E+3 6.928E+2 1.020E+3 7.235E+2 7.683E+2 2.449E+2
N_corr 1.432E+3 8.846E+2 1.206E+3 5.026E+2 6.208E+2 4.641E+2 1.075E+3 6.843E+2 9.026E+2 6.501E+2 8.158E+2 2.692E+2
c [nM] 8.709E+3 5.391E+3 6.523E+3 2.845E+3 3.112E+3 2.340E+3 6.580E+3 4.309E+3 4.733E+3 3.356E+3 3.950E+3 1.259E+3
c_corr [nM] 8.906E+3 5.502E+3 5.596E+3 2.331E+3 3.192E+3 2.386E+3 6.683E+3 4.256E+3 4.186E+3 3.015E+3 4.194E+3 1.384E+3
ratioG 3.794E-1 1.560E-1 4.915E-1 1.536E-2
ratioG_corr 9.983E-2 1.090E-1 2.061E-1 1.865E-3
f_1 9.352E-1 4.225E-2 5.324E-1 2.127E-1 9.958E-1 1.347E-2 8.582E-1 8.419E-2 5.064E-1 3.100E-1 1.000E+0 0.000E+0
tauD_1 [ms] 3.783E-1 2.065E-1 1.860E+0 2.220E-16 2.440E-1 2.175E-1 6.441E-1 9.558E-1 1.860E+0 2.220E-16 1.130E-1 1.303E-2
D_1 [mum^2/s] 3.459E+1 1.458E+1 6.926E+0 8.882E-16 7.497E+1 3.743E+1 2.609E+1 1.522E+1 6.926E+0 8.882E-16 1.075E+2 1.239E+1
alpha_1 1.125E+0 9.852E-2 9.200E-1 1.110E-16 1.127E+0 1.251E-1 1.121E+0 1.059E-1 9.200E-1 0.000E+0 9.752E-1 2.248E-1
f_2 6.478E-2 4.225E-2 4.676E-1 2.127E-1 4.216E-3 1.347E-2 1.418E-1 8.419E-2 4.936E-1 3.100E-1 0.000E+0 0.000E+0
tauD_2 [ms] 1.482E+1 1.892E+1 6.162E+1 6.334E+1 4.321E+1 4.396E+1 4.335E+1 7.289E+1 3.086E+1 2.435E+1 2.666E+1 6.303E+0
D_2 [mum^2/s] 1.132E+0 5.300E-1 4.768E-1 5.516E-1 4.103E-1 2.145E-1 5.855E-1 3.742E-1 9.945E-1 1.118E+0 4.764E-1 1.127E-1
alpha_2 1.109E+0 9.755E-2 1.376E+0 3.602E-1 1.104E+0 1.209E-1 1.132E+0 2.580E-1 1.312E+0 3.454E-1 1.081E+0 2.300E-3
tauD_m [ms] 1.509E+0 2.286E+0 3.058E+1 3.678E+1 7.967E-1 2.191E+0 9.551E+0 2.264E+1 1.436E+1 9.513E+0 1.130E-1 1.303E-2
D_m [mum^2/s] 3.254E+1 1.421E+1 3.927E+0 1.343E+0 7.477E+1 3.756E+1 2.298E+1 1.510E+1 3.943E+0 2.015E+0 1.075E+2 1.239E+1
R [1/s] 1.079E+2 6.256E+1 1.104E+2 3.298E+0
CPM [kHz] 4.128E-1 1.479E-1 2.070E-1 1.179E-1 5.333E-1 2.317E-1 1.713E-1 7.113E-2
CPM_corr [kHz] 4.150E-1 1.482E-1 3.214E-1 1.825E-1 5.389E-1 2.344E-1 5.577E-1 4.603E-1
Int [kHz] 5.938E+2 3.153E+2 1.936E+2 4.887E+1 5.197E+2 3.273E+2 1.567E+2 1.053E+2
Int_corr [kHz] 6.096E+2 3.236E+2 2.629E+2 6.235E+1 5.274E+2 3.204E+2 2.223E+2 1.545E+2
# samples 149 149 89 89 38 38 59 59 8 8 2 2
# cells 88

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 8905.641 - sd = 5501.840Int_corr [kHz] - Ch1 - Nuc : ave = 609.609 - sd = 323.562r = 0.832 - r^2 = 0.692
f_1 - Ch1 - Nuc : ave = 0.935 - sd = 0.042D_1 [mum^2/s] - Ch1 - Nuc : ave = 34.589 - sd = 14.584r = 0.007 - r^2 = 5.015E-5
f_2 - Ch1 - Nuc : ave = 0.065 - sd = 0.042D_2 [mum^2/s] - Ch1 - Nuc : ave = 1.132 - sd = 0.530r = -0.457 - r^2 = 0.209
CPM_corr [kHz] - Ch1 - Nuc : ave = 0.415 - sd = 0.148D_m [mum^2/s] - Ch1 - Nuc : ave = 32.542 - sd = 14.206r = 0.158 - r^2 = 0.025
c_corr [nM] - Ch1 - Nuc : ave = 8905.641 - sd = 5501.840ratioG_corr - Cross - Nuc : ave = 0.100 - sd = 0.109r = 0.082 - r^2 = 0.007
c_corr [nM] - Ch1 - Cyto : ave = 6682.877 - sd = 4255.798Int_corr [kHz] - Ch1 - Cyto : ave = 527.418 - sd = 320.404r = 0.919 - r^2 = 0.844
f_1 - Ch1 - Cyto : ave = 0.858 - sd = 0.084D_1 [mum^2/s] - Ch1 - Cyto : ave = 26.092 - sd = 15.221r = 0.347 - r^2 = 0.121
f_2 - Ch1 - Cyto : ave = 0.142 - sd = 0.084D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.586 - sd = 0.374r = -0.703 - r^2 = 0.495
CPM_corr [kHz] - Ch1 - Cyto : ave = 0.539 - sd = 0.234D_m [mum^2/s] - Ch1 - Cyto : ave = 22.985 - sd = 15.104r = -0.264 - r^2 = 0.070