Automated F(C)CS analysis of clone # DDIT3G06

Created on 12/08/2014
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Gene and protein information

Clone # DDIT3G06
Gene symbol: DDIT3
Description: DNA-damage-inducible transcript 3
Molecular weight (with FP): 46.2 kDa
Localisation: homogeneous nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
DDIT3G06_img_ch1.pngDDIT3G06_img_ch2.png


Point 1, intensity traces and correlation functions
DDIT3G06_itr_1.pngDDIT3G06_cor_1.png

Point 2, intensity traces and correlation functions
DDIT3G06_itr_2.pngDDIT3G06_cor_2.png

Point 3, intensity traces and correlation functions
DDIT3G06_itr_3.pngDDIT3G06_cor_3.png

Point 4, intensity traces and correlation functions
DDIT3G06_itr_4.pngDDIT3G06_cor_4.png

Point 5, intensity traces and correlation functions
DDIT3G06_itr_5.pngDDIT3G06_cor_5.png

Point 6, intensity traces and correlation functions
DDIT3G06_itr_6.pngDDIT3G06_cor_6.png

Sessions

1 20120202\U02--V00--DDIT3G06
2 20120207\U02--V00--DDIT3G06

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 7.951E+1 3.386E+1 9.112E+2 4.466E+2 1.209E+1 8.130E+0 2.158E+1 2.949E+1 1.443E+2 1.713E+2 7.152E+0 4.947E+0
N_corr 8.272E+1 3.624E+1 1.155E+3 6.619E+2 1.326E+1 9.194E+0 2.129E+1 3.030E+1 1.463E+2 2.516E+2 8.751E+0 5.960E+0
c [nM] 4.945E+2 2.106E+2 4.226E+3 2.071E+3 6.218E+1 4.180E+1 1.342E+2 1.834E+2 6.692E+2 7.945E+2 3.677E+1 2.543E+1
c_corr [nM] 5.144E+2 2.254E+2 5.356E+3 3.070E+3 6.818E+1 4.727E+1 1.324E+2 1.884E+2 6.786E+2 1.167E+3 4.499E+1 3.064E+1
ratioG 1.330E-1 5.130E-2 3.779E-1 1.787E-1
ratioG_corr 9.304E-3 2.590E-2 7.969E-2 5.454E-2
f_1 8.821E-1 1.015E-1 4.547E-1 1.913E-1 9.806E-1 4.553E-2 8.975E-1 6.566E-2 8.636E-1 2.263E-1 9.783E-1 4.276E-2
tauD_1 [ms] 2.720E+0 1.150E+0 1.860E+0 2.220E-16 1.763E+0 7.054E-1 1.230E+0 6.985E-1 1.860E+0 2.220E-16 8.009E-1 6.663E-1
D_1 [mum^2/s] 4.344E+0 1.305E+0 6.926E+0 0.000E+0 8.182E+0 4.194E+0 1.152E+1 9.637E+0 6.926E+0 0.000E+0 2.527E+1 1.737E+1
alpha_1 9.776E-1 8.236E-2 9.200E-1 2.220E-16 8.533E-1 1.794E-1 9.448E-1 1.031E-1 9.200E-1 1.110E-16 7.766E-1 1.708E-1
f_2 1.179E-1 1.015E-1 5.453E-1 1.913E-1 1.944E-2 4.553E-2 1.025E-1 6.566E-2 1.364E-1 2.263E-1 2.174E-2 4.276E-2
tauD_2 [ms] 3.071E+1 1.573E+1 8.482E+1 9.525E+1 5.464E+1 3.716E+1 2.468E+1 1.768E+1 4.708E+1 4.723E+1 3.004E+1 2.079E+1
D_2 [mum^2/s] 4.032E-1 1.389E-1 6.155E-1 9.642E-1 2.783E-1 1.092E-1 5.676E-1 2.657E-1 8.126E-1 1.529E+0 5.393E-1 2.767E-1
alpha_2 1.335E+0 2.643E-1 1.287E+0 3.324E-1 1.133E+0 2.170E-1 1.167E+0 1.713E-1 1.169E+0 2.988E-1 1.113E+0 1.475E-1
tauD_m [ms] 6.236E+0 4.287E+0 4.416E+1 5.419E+1 2.884E+0 3.560E+0 3.584E+0 3.085E+0 6.581E+0 9.091E+0 1.329E+0 1.399E+0
D_m [mum^2/s] 3.880E+0 1.211E+0 3.572E+0 1.099E+0 8.055E+0 4.221E+0 1.015E+1 6.862E+0 6.279E+0 9.941E-1 2.480E+1 1.707E+1
R [1/s] 2.102E+1 2.026E+1 2.902E+1 3.076E+1
CPM [kHz] 2.377E+0 6.476E-1 3.683E-1 3.034E-1 1.499E+0 7.432E-1 2.058E-1 1.319E-1
CPM_corr [kHz] 2.405E+0 6.436E-1 4.599E-1 3.655E-1 1.696E+0 7.892E-1 6.042E-1 6.316E-1
Int [kHz] 2.086E+2 1.118E+2 1.646E+2 5.209E+1 2.582E+1 2.470E+1 3.324E+1 4.590E+1
Int_corr [kHz] 2.190E+2 1.181E+2 2.586E+2 9.535E+1 2.813E+1 2.631E+1 4.869E+1 8.001E+1
# samples 216 216 162 162 162 162 167 167 53 53 43 43
# cells 74

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 514.447 - sd = 225.398Int_corr [kHz] - Ch1 - Nuc : ave = 218.983 - sd = 118.055r = 0.853 - r^2 = 0.728
f_1 - Ch1 - Nuc : ave = 0.882 - sd = 0.102D_1 [mum^2/s] - Ch1 - Nuc : ave = 4.344 - sd = 1.305r = 0.101 - r^2 = 0.010
f_2 - Ch1 - Nuc : ave = 0.118 - sd = 0.102D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.403 - sd = 0.139r = -0.289 - r^2 = 0.084
CPM_corr [kHz] - Ch1 - Nuc : ave = 2.405 - sd = 0.644D_m [mum^2/s] - Ch1 - Nuc : ave = 3.880 - sd = 1.211r = 0.250 - r^2 = 0.063
c_corr [nM] - Ch1 - Nuc : ave = 514.447 - sd = 225.398ratioG_corr - Cross - Nuc : ave = 0.009 - sd = 0.026r = 0.062 - r^2 = 0.004
c_corr [nM] - Ch1 - Cyto : ave = 132.407 - sd = 188.442Int_corr [kHz] - Ch1 - Cyto : ave = 28.130 - sd = 26.309r = 0.553 - r^2 = 0.306
f_1 - Ch1 - Cyto : ave = 0.897 - sd = 0.066D_1 [mum^2/s] - Ch1 - Cyto : ave = 11.524 - sd = 9.637r = -0.293 - r^2 = 0.086
f_2 - Ch1 - Cyto : ave = 0.103 - sd = 0.066D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.568 - sd = 0.266r = -0.032 - r^2 = 0.001
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.696 - sd = 0.789D_m [mum^2/s] - Ch1 - Cyto : ave = 10.155 - sd = 6.862r = 0.210 - r^2 = 0.044