Automated F(C)CS analysis of clone # CENPAC07hi

Created on 12/08/2014
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Gene and protein information

Clone # CENPAC07hi
Gene symbol: CENPA
Description: centromere protein A
Molecular weight (with FP): 43.0 kDa
Localisation: granular nucleus, ave. nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
CENPAC07hi_img_ch1.pngCENPAC07hi_img_ch2.png


Point 1, intensity traces and correlation functions
CENPAC07hi_itr_1.pngCENPAC07hi_cor_1.png

Point 2, intensity traces and correlation functions
CENPAC07hi_itr_2.pngCENPAC07hi_cor_2.png

Point 3, intensity traces and correlation functions
CENPAC07hi_itr_3.pngCENPAC07hi_cor_3.png

Point 4, intensity traces and correlation functions
CENPAC07hi_itr_4.pngCENPAC07hi_cor_4.png

Point 5, intensity traces and correlation functions
CENPAC07hi_itr_5.pngCENPAC07hi_cor_5.png

Point 6, intensity traces and correlation functions
CENPAC07hi_itr_6.pngCENPAC07hi_cor_6.png

Sessions

1 20130109\U06--V00--CENPAC07hi
2 20130111\U00--V01--CENPAC07hi

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.456E+2 1.017E+2 6.065E+2 2.817E+2 3.648E+1 3.995E+1 3.062E+1 5.346E+1 2.659E+2 2.486E+2 5.862E+0 4.574E+0
N_corr 3.106E+2 2.653E+2 9.837E+2 5.488E+2 2.093E+2 2.582E+2 4.398E+1 1.003E+2 4.321E+2 4.781E+2 2.657E+1 2.261E+1
c [nM] 9.055E+2 6.327E+2 2.813E+3 1.307E+3 1.875E+2 2.054E+2 1.904E+2 3.325E+2 1.234E+3 1.153E+3 3.014E+1 2.351E+1
c_corr [nM] 1.932E+3 1.650E+3 4.563E+3 2.546E+3 1.076E+3 1.327E+3 2.735E+2 6.240E+2 2.004E+3 2.218E+3 1.366E+2 1.162E+2
ratioG 1.961E-1 1.146E-1 1.621E-1 9.538E-2
ratioG_corr 3.584E-1 2.617E-1 2.063E-1 1.478E-1
f_1 8.071E-1 1.742E-1 3.824E-1 2.148E-1 7.238E-1 2.313E-1 8.943E-1 8.878E-2 5.065E-1 3.554E-1 8.908E-1 1.392E-1
tauD_1 [ms] 3.463E+0 3.977E+0 1.860E+0 4.441E-16 7.210E+0 1.162E+1 1.175E+0 1.248E+0 1.860E+0 2.220E-16 4.352E+0 2.331E+0
D_1 [mum^2/s] 5.957E+0 6.207E+0 6.926E+0 3.553E-15 8.271E+0 1.527E+1 1.922E+1 1.457E+1 6.926E+0 8.882E-16 3.628E+0 1.729E+0
alpha_1 9.536E-1 1.484E-1 9.200E-1 7.772E-16 9.186E-1 2.436E-1 1.018E+0 1.590E-1 9.200E-1 1.110E-16 1.074E+0 9.935E-2
f_2 1.929E-1 1.742E-1 6.176E-1 2.148E-1 2.762E-1 2.313E-1 1.057E-1 8.878E-2 4.935E-1 3.554E-1 1.092E-1 1.392E-1
tauD_2 [ms] 6.607E+1 7.157E+1 8.713E+1 8.423E+1 1.617E+2 1.958E+2 2.598E+1 1.502E+1 5.201E+1 7.442E+1 9.019E+1 2.525E+1
D_2 [mum^2/s] 2.557E-1 1.261E-1 4.518E-1 6.447E-1 1.332E-1 8.450E-2 5.495E-1 3.069E-1 1.373E+0 1.706E+0 1.451E-1 4.432E-2
alpha_2 1.260E+0 2.858E-1 1.458E+0 4.042E-1 1.499E+0 4.076E-1 1.179E+0 1.914E-1 1.470E+0 4.120E-1 1.389E+0 4.320E-1
tauD_m [ms] 1.657E+1 2.412E+1 5.334E+1 5.925E+1 5.394E+1 7.675E+1 3.961E+0 3.590E+0 2.524E+1 5.102E+1 1.374E+1 1.215E+1
D_m [mum^2/s] 4.650E+0 4.099E+0 2.975E+0 1.306E+0 5.893E+0 1.128E+1 1.731E+1 1.347E+1 4.605E+0 1.704E+0 3.276E+0 1.832E+0
R [1/s] 6.838E+0 1.084E+1 1.424E+1 1.858E+1
CPM [kHz] 5.544E-1 1.107E+0 4.770E-1 3.492E-1 3.853E-1 5.994E-1 2.797E-1 1.757E-1
CPM_corr [kHz] 5.747E-1 1.110E+0 5.141E-1 3.737E-1 9.085E+0 3.966E+1 3.142E-1 2.396E-1
Int [kHz] 5.662E+1 7.461E+1 1.619E+2 4.028E+1 1.057E+1 1.731E+1 6.358E+1 5.823E+1
Int_corr [kHz] 1.108E+2 1.146E+2 2.730E+2 7.927E+1 1.978E+1 4.451E+1 1.211E+2 1.178E+2
# samples 320 320 275 275 208 208 71 71 9 9 3 3
# cells 150

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 1931.938 - sd = 1649.722Int_corr [kHz] - Ch1 - Nuc : ave = 110.835 - sd = 114.554r = 0.716 - r^2 = 0.513
f_1 - Ch1 - Nuc : ave = 0.807 - sd = 0.174D_1 [mum^2/s] - Ch1 - Nuc : ave = 5.957 - sd = 6.207r = -0.141 - r^2 = 0.020
f_2 - Ch1 - Nuc : ave = 0.193 - sd = 0.174D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.256 - sd = 0.126r = 0.040 - r^2 = 0.002
CPM_corr [kHz] - Ch1 - Nuc : ave = 0.575 - sd = 1.110D_m [mum^2/s] - Ch1 - Nuc : ave = 4.650 - sd = 4.099r = -0.104 - r^2 = 0.011
c_corr [nM] - Ch1 - Nuc : ave = 1931.938 - sd = 1649.722ratioG_corr - Cross - Nuc : ave = 0.358 - sd = 0.262r = 0.801 - r^2 = 0.641
c_corr [nM] - Ch1 - Cyto : ave = 273.532 - sd = 623.952Int_corr [kHz] - Ch1 - Cyto : ave = 19.780 - sd = 44.509r = 0.951 - r^2 = 0.904
f_1 - Ch1 - Cyto : ave = 0.894 - sd = 0.089D_1 [mum^2/s] - Ch1 - Cyto : ave = 19.218 - sd = 14.572r = 0.079 - r^2 = 0.006
f_2 - Ch1 - Cyto : ave = 0.106 - sd = 0.089D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.550 - sd = 0.307r = -0.090 - r^2 = 0.008
CPM_corr [kHz] - Ch1 - Cyto : ave = 9.085 - sd = 39.657D_m [mum^2/s] - Ch1 - Cyto : ave = 17.306 - sd = 13.472r = -0.150 - r^2 = 0.023