Automated F(C)CS analysis of clone # CENPAC07

Created on 12/08/2014
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Gene and protein information

Clone # CENPAC07
Gene symbol: CENPA
Description: centromere protein A
Molecular weight (with FP): 43.0 kDa
Localisation: granular nucleus, ave. nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
CENPAC07_img_ch1.pngCENPAC07_img_ch2.png


Point 1, intensity traces and correlation functions
CENPAC07_itr_1.pngCENPAC07_cor_1.png

Point 2, intensity traces and correlation functions
CENPAC07_itr_2.pngCENPAC07_cor_2.png

Point 3, intensity traces and correlation functions
CENPAC07_itr_3.pngCENPAC07_cor_3.png

Point 4, intensity traces and correlation functions
CENPAC07_itr_4.pngCENPAC07_cor_4.png

Point 5, intensity traces and correlation functions
CENPAC07_itr_5.pngCENPAC07_cor_5.png

Point 6, intensity traces and correlation functions
CENPAC07_itr_6.pngCENPAC07_cor_6.png

Sessions

1 20120203\U00--V00--CENPAC07
2 20120221\U02--V00--CENPAC07

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.005E+2 6.516E+1 1.012E+3 4.800E+2 1.048E+1 1.252E+1 2.341E+1 2.958E+1 2.943E+2 1.904E+2 1.450E+1 1.984E+1
N_corr 1.520E+2 1.269E+2 1.537E+3 7.913E+2 2.129E+1 2.949E+1 2.742E+1 4.768E+1 3.739E+2 2.921E+2 4.059E+1 8.412E+1
c [nM] 6.249E+2 4.052E+2 4.692E+3 2.226E+3 5.387E+1 6.434E+1 1.456E+2 1.840E+2 1.365E+3 8.833E+2 7.455E+1 1.020E+2
c_corr [nM] 9.451E+2 7.892E+2 7.129E+3 3.670E+3 1.094E+2 1.516E+2 1.705E+2 2.965E+2 1.734E+3 1.355E+3 2.087E+2 4.325E+2
ratioG 9.279E-2 5.779E-2 2.577E-1 1.597E-1
ratioG_corr 2.311E-2 5.371E-2 1.991E-1 2.421E-1
f_1 8.106E-1 1.538E-1 4.383E-1 2.158E-1 9.520E-1 1.018E-1 8.921E-1 8.474E-2 7.129E-1 3.586E-1 9.181E-1 1.145E-1
tauD_1 [ms] 2.619E+0 2.616E+0 1.860E+0 2.220E-16 2.083E+0 1.956E+0 1.064E+0 1.209E+0 1.860E+0 0.000E+0 1.795E+0 1.269E+0
D_1 [mum^2/s] 6.142E+0 3.729E+0 6.926E+0 8.882E-16 1.148E+1 1.587E+1 1.853E+1 1.443E+1 6.926E+0 0.000E+0 2.163E+1 3.296E+1
alpha_1 9.346E-1 9.898E-2 9.200E-1 0.000E+0 8.014E-1 2.242E-1 9.809E-1 1.111E-1 9.200E-1 0.000E+0 1.041E+0 1.907E-1
f_2 1.894E-1 1.538E-1 5.617E-1 2.158E-1 4.805E-2 1.018E-1 1.079E-1 8.474E-2 2.871E-1 3.586E-1 8.190E-2 1.145E-1
tauD_2 [ms] 3.714E+1 3.039E+1 9.316E+1 1.125E+2 1.180E+2 1.090E+2 2.058E+1 1.494E+1 6.663E+1 8.760E+1 5.167E+1 3.081E+1
D_2 [mum^2/s] 4.059E-1 1.980E-1 4.908E-1 7.188E-1 1.610E-1 8.776E-2 7.206E-1 4.027E-1 1.506E+0 2.703E+0 4.491E-1 5.232E-1
alpha_2 1.366E+0 2.861E-1 1.310E+0 3.065E-1 1.125E+0 1.671E-1 1.138E+0 1.497E-1 1.301E+0 4.114E-1 1.152E+0 2.719E-1
tauD_m [ms] 9.675E+0 9.659E+0 5.163E+1 6.239E+1 1.338E+1 3.852E+1 3.622E+0 4.829E+0 1.033E+1 1.267E+1 8.192E+0 1.405E+1
D_m [mum^2/s] 4.988E+0 2.674E+0 3.358E+0 1.329E+0 1.083E+1 1.441E+1 1.661E+1 1.335E+1 5.869E+0 1.394E+0 2.045E+1 3.167E+1
R [1/s] 2.136E+1 2.028E+1 3.393E+1 4.385E+1
CPM [kHz] 1.543E+0 5.184E-1 4.422E-1 4.368E-1 8.340E-1 5.614E-1 2.154E-1 1.134E-1
CPM_corr [kHz] 1.569E+0 5.136E-1 5.043E-1 4.810E-1 1.139E+0 7.174E-1 3.136E-1 2.035E-1
Int [kHz] 1.547E+2 9.873E+1 1.803E+2 5.551E+1 1.477E+1 1.500E+1 5.519E+1 3.427E+1
Int_corr [kHz] 2.263E+2 1.574E+2 3.137E+2 1.133E+2 1.867E+1 2.053E+1 8.345E+1 6.278E+1
# samples 192 192 140 140 131 131 142 142 24 24 19 19
# cells 66

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 945.078 - sd = 789.240Int_corr [kHz] - Ch1 - Nuc : ave = 226.335 - sd = 157.366r = 0.817 - r^2 = 0.668
f_1 - Ch1 - Nuc : ave = 0.811 - sd = 0.154D_1 [mum^2/s] - Ch1 - Nuc : ave = 6.142 - sd = 3.729r = 0.075 - r^2 = 0.006
f_2 - Ch1 - Nuc : ave = 0.189 - sd = 0.154D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.406 - sd = 0.198r = -0.240 - r^2 = 0.058
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.569 - sd = 0.514D_m [mum^2/s] - Ch1 - Nuc : ave = 4.988 - sd = 2.674r = -0.273 - r^2 = 0.075
c_corr [nM] - Ch1 - Nuc : ave = 945.078 - sd = 789.240ratioG_corr - Cross - Nuc : ave = 0.023 - sd = 0.054r = -0.117 - r^2 = 0.014
c_corr [nM] - Ch1 - Cyto : ave = 170.531 - sd = 296.526Int_corr [kHz] - Ch1 - Cyto : ave = 18.667 - sd = 20.526r = 0.726 - r^2 = 0.527
f_1 - Ch1 - Cyto : ave = 0.892 - sd = 0.085D_1 [mum^2/s] - Ch1 - Cyto : ave = 18.533 - sd = 14.431r = -0.013 - r^2 = 0.000
f_2 - Ch1 - Cyto : ave = 0.108 - sd = 0.085D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.721 - sd = 0.403r = 0.166 - r^2 = 0.028
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.139 - sd = 0.717D_m [mum^2/s] - Ch1 - Cyto : ave = 16.612 - sd = 13.346r = 0.083 - r^2 = 0.007