Automated F(C)CS analysis of clone # 3163

Created on 12/08/2014
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Gene and protein information

Clone # 3163
Gene symbol: GADD45A
Description: growth arrest and DNA-damage-inducible, alpha
Molecular weight (with FP): 45.3 kDa
Localisation: homogeneous nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
3163_img_ch1.png3163_img_ch2.png


Point 1, intensity traces and correlation functions
3163_itr_1.png3163_cor_1.png

Point 2, intensity traces and correlation functions
3163_itr_2.png3163_cor_2.png

Point 3, intensity traces and correlation functions
3163_itr_3.png3163_cor_3.png

Point 4, intensity traces and correlation functions
3163_itr_4.png3163_cor_4.png

Point 5, intensity traces and correlation functions
3163_itr_5.png3163_cor_5.png

Point 6, intensity traces and correlation functions
3163_itr_6.png3163_cor_6.png

Sessions

1 20120217\U01--V00--3163
2 20120306\U02--V00--3163
3 20120307\U00--V00--3163
4 20120308\U02--V00--3163
5 20120315\U01--V00--3163

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 6.879E+1 2.788E+1 1.273E+3 5.776E+2 4.644E+0 2.691E+0 7.134E+1 3.820E+1 1.607E+2 1.592E+2 3.609E+1 2.118E+1
N_corr 6.957E+1 2.917E+1 2.058E+3 1.058E+3 4.818E+0 2.873E+0 7.419E+1 3.941E+1 1.231E+2 3.206E+2 4.176E+1 2.466E+1
c [nM] 4.278E+2 1.734E+2 5.904E+3 2.679E+3 2.387E+1 1.383E+1 4.437E+2 2.376E+2 7.452E+2 7.383E+2 1.855E+2 1.089E+2
c_corr [nM] 4.327E+2 1.814E+2 9.546E+3 4.907E+3 2.477E+1 1.477E+1 4.614E+2 2.451E+2 5.712E+2 1.487E+3 2.147E+2 1.268E+2
ratioG 6.061E-2 2.016E-2 5.972E-1 2.004E-1
ratioG_corr 3.797E-3 1.380E-2 1.098E-1 6.605E-2
f_1 9.245E-1 5.619E-2 3.597E-1 2.081E-1 9.844E-1 3.793E-2 9.300E-1 7.311E-2 9.206E-1 2.056E-1 9.800E-1 4.145E-2
tauD_1 [ms] 7.643E-1 3.693E-1 1.860E+0 2.220E-16 5.361E-1 3.912E-1 8.930E-1 1.157E+0 1.860E+0 2.220E-16 4.158E-1 2.443E-1
D_1 [mum^2/s] 1.591E+1 4.684E+0 6.926E+0 0.000E+0 3.770E+1 3.047E+1 1.614E+1 5.784E+0 6.926E+0 8.882E-16 3.352E+1 1.022E+1
alpha_1 9.519E-1 8.718E-2 9.200E-1 0.000E+0 9.246E-1 2.195E-1 9.692E-1 1.210E-1 9.200E-1 0.000E+0 8.174E-1 1.412E-1
f_2 7.550E-2 5.619E-2 6.403E-1 2.081E-1 1.561E-2 3.793E-2 6.998E-2 7.311E-2 7.939E-2 2.056E-1 2.001E-2 4.145E-2
tauD_2 [ms] 1.316E+1 4.620E+0 8.906E+1 1.098E+2 5.881E+1 4.067E+1 2.508E+1 5.760E+1 2.555E+1 2.610E+1 1.822E+1 1.045E+1
D_2 [mum^2/s] 8.865E-1 2.518E-1 5.076E-1 6.103E-1 2.591E-1 1.056E-1 7.211E-1 3.261E-1 1.226E+0 2.503E+0 8.116E-1 3.295E-1
alpha_2 1.157E+0 1.177E-1 1.222E+0 2.577E-1 1.076E+0 5.349E-2 1.086E+0 1.010E-1 1.104E+0 1.614E-1 1.083E+0 3.570E-2
tauD_m [ms] 1.751E+0 1.102E+0 5.931E+1 8.521E+1 2.091E+0 6.017E+0 4.593E+0 2.836E+1 4.224E+0 1.357E+1 7.432E-1 7.429E-1
D_m [mum^2/s] 1.479E+1 4.389E+0 2.850E+0 1.294E+0 3.713E+1 3.011E+1 1.497E+1 5.239E+0 6.687E+0 1.117E+0 3.280E+1 1.003E+1
R [1/s] 7.429E+1 4.129E+1 2.531E+1 2.934E+1
CPM [kHz] 1.371E+0 2.359E-1 2.889E-1 2.778E-1 1.377E+0 5.467E-1 1.460E-1 5.919E-2
CPM_corr [kHz] 1.401E+0 2.367E-1 3.193E-1 3.053E-1 1.408E+0 5.528E-1 6.860E-1 3.913E-1
Int [kHz] 1.070E+2 4.447E+1 1.825E+2 5.080E+1 9.514E+1 4.544E+1 2.755E+1 2.939E+1
Int_corr [kHz] 1.101E+2 4.626E+1 3.227E+2 1.041E+2 1.012E+2 4.741E+1 3.808E+1 5.932E+1
# samples 180 180 123 123 112 112 151 151 65 65 62 62
# cells 65

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 432.700 - sd = 181.400Int_corr [kHz] - Ch1 - Nuc : ave = 110.136 - sd = 46.264r = 0.834 - r^2 = 0.696
f_1 - Ch1 - Nuc : ave = 0.924 - sd = 0.056D_1 [mum^2/s] - Ch1 - Nuc : ave = 15.912 - sd = 4.684r = -0.090 - r^2 = 0.008
f_2 - Ch1 - Nuc : ave = 0.076 - sd = 0.056D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.887 - sd = 0.252r = -0.023 - r^2 = 0.001
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.401 - sd = 0.237D_m [mum^2/s] - Ch1 - Nuc : ave = 14.790 - sd = 4.389r = 0.313 - r^2 = 0.098
c_corr [nM] - Ch1 - Nuc : ave = 432.700 - sd = 181.400ratioG_corr - Cross - Nuc : ave = 0.004 - sd = 0.014r = 0.079 - r^2 = 0.006
c_corr [nM] - Ch1 - Cyto : ave = 461.417 - sd = 245.075Int_corr [kHz] - Ch1 - Cyto : ave = 101.167 - sd = 47.414r = 0.335 - r^2 = 0.112
f_1 - Ch1 - Cyto : ave = 0.930 - sd = 0.073D_1 [mum^2/s] - Ch1 - Cyto : ave = 16.137 - sd = 5.784r = -0.266 - r^2 = 0.071
f_2 - Ch1 - Cyto : ave = 0.070 - sd = 0.073D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.721 - sd = 0.326r = 0.214 - r^2 = 0.046
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.408 - sd = 0.553D_m [mum^2/s] - Ch1 - Cyto : ave = 14.969 - sd = 5.239r = 0.397 - r^2 = 0.157