Automated F(C)CS analysis of clone # 3155hi

Created on 12/08/2014
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Gene and protein information

Clone # 3155hi
Gene symbol: GATA3
Description: GATA binding protein 3
Molecular weight (with FP): 74.9 kDa
Localisation: granular nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
3155hi_img_ch1.png3155hi_img_ch2.png


Point 1, intensity traces and correlation functions
3155hi_itr_1.png3155hi_cor_1.png

Point 2, intensity traces and correlation functions
3155hi_itr_2.png3155hi_cor_2.png

Point 3, intensity traces and correlation functions
3155hi_itr_3.png3155hi_cor_3.png

Point 4, intensity traces and correlation functions
3155hi_itr_4.png3155hi_cor_4.png

Point 5, intensity traces and correlation functions
3155hi_itr_5.png3155hi_cor_5.png

Point 6, intensity traces and correlation functions
3155hi_itr_6.png3155hi_cor_6.png

Sessions

1 20130125\U03--V00--3155hi
2 20130125\U03--V01--3155hi
3 20130131\U02--V00--3155hi
4 20130131\U02--V01--3155hi
5 20130201\U02--V00--3155hi

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.553E+2 9.835E+1 6.609E+2 3.163E+2 5.245E+1 5.560E+1 4.521E+1 8.043E+1 2.731E+2 1.220E+2 2.227E+1 8.018E+0
N_corr 1.749E+2 1.225E+2 1.074E+3 5.562E+2 7.497E+1 9.029E+1 4.029E+1 8.134E+1 3.863E+2 2.224E+2 2.406E+1 1.021E+1
c [nM] 9.661E+2 6.117E+2 3.065E+3 1.467E+3 2.696E+2 2.858E+2 2.812E+2 5.002E+2 1.267E+3 5.661E+2 1.145E+2 4.122E+1
c_corr [nM] 1.088E+3 7.616E+2 4.984E+3 2.580E+3 3.854E+2 4.642E+2 2.506E+2 5.059E+2 1.792E+3 1.032E+3 1.237E+2 5.247E+1
ratioG 2.293E-1 1.137E-1 2.888E-1 1.436E-1
ratioG_corr 1.798E-1 1.035E-1 2.537E-1 1.256E-1
f_1 6.523E-1 2.171E-1 2.708E-1 1.691E-1 8.199E-1 1.922E-1 8.463E-1 1.943E-1 4.628E-1 2.003E-1 8.163E-1 2.172E-1
tauD_1 [ms] 6.143E+0 5.338E+0 1.860E+0 0.000E+0 9.744E+0 8.992E+0 3.187E+0 7.137E+0 1.860E+0 0.000E+0 1.496E+1 1.225E+1
D_1 [mum^2/s] 4.224E+0 4.097E+0 6.926E+0 0.000E+0 3.115E+0 3.490E+0 2.367E+1 2.286E+1 6.926E+0 0.000E+0 1.787E+0 1.443E+0
alpha_1 9.457E-1 1.113E-1 9.200E-1 1.110E-16 9.102E-1 2.017E-1 1.044E+0 1.705E-1 9.200E-1 0.000E+0 1.103E+0 1.375E-1
f_2 3.477E-1 2.171E-1 7.292E-1 1.691E-1 1.801E-1 1.922E-1 1.537E-1 1.943E-1 5.372E-1 2.003E-1 1.837E-1 2.172E-1
tauD_2 [ms] 6.972E+1 4.348E+1 5.580E+1 3.223E+1 1.343E+2 7.534E+1 2.950E+1 2.697E+1 6.427E+1 8.129E+0 1.452E+2 4.683E+1
D_2 [mum^2/s] 1.912E-1 8.210E-2 3.503E-1 3.222E-1 1.200E-1 6.684E-2 6.085E-1 3.572E-1 2.041E-1 2.879E-2 9.397E-2 3.598E-2
alpha_2 1.506E+0 3.401E-1 1.396E+0 3.388E-1 1.466E+0 4.304E-1 1.219E+0 2.841E-1 1.390E+0 3.880E-1 1.395E+0 4.283E-1
tauD_m [ms] 2.515E+1 1.103E+1 3.978E+1 2.355E+1 2.364E+1 1.344E+1 8.204E+0 1.187E+1 3.608E+1 1.504E+1 3.143E+1 1.525E+1
D_m [mum^2/s] 2.213E+0 1.659E+0 2.152E+0 1.042E+0 2.258E+0 2.252E+0 2.216E+1 2.269E+1 3.312E+0 1.355E+0 1.190E+0 6.701E-1
R [1/s] 4.046E+0 7.906E+0 1.190E+0 1.683E+0
CPM [kHz] 4.016E-1 1.804E-1 3.263E-1 2.815E-1 2.250E-1 1.386E-1 5.178E-1 3.635E-1
CPM_corr [kHz] 4.183E-1 1.784E-1 3.644E-1 3.027E-1 8.811E+0 4.374E+1 5.756E-1 4.313E-1
Int [kHz] 6.561E+1 6.331E+1 1.151E+2 3.221E+1 1.105E+1 1.737E+1 7.222E+1 1.493E+1
Int_corr [kHz] 7.769E+1 8.348E+1 2.105E+2 7.229E+1 1.170E+1 1.827E+1 1.093E+2 3.879E+1
# samples 59 59 46 46 27 27 39 39 4 4 3 3
# cells 30

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 1087.651 - sd = 761.556Int_corr [kHz] - Ch1 - Nuc : ave = 77.694 - sd = 83.483r = 0.780 - r^2 = 0.608
f_1 - Ch1 - Nuc : ave = 0.652 - sd = 0.217D_1 [mum^2/s] - Ch1 - Nuc : ave = 4.224 - sd = 4.097r = -0.714 - r^2 = 0.510
f_2 - Ch1 - Nuc : ave = 0.348 - sd = 0.217D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.191 - sd = 0.082r = 0.215 - r^2 = 0.046
CPM_corr [kHz] - Ch1 - Nuc : ave = 0.418 - sd = 0.178D_m [mum^2/s] - Ch1 - Nuc : ave = 2.213 - sd = 1.659r = 0.079 - r^2 = 0.006
c_corr [nM] - Ch1 - Nuc : ave = 1087.651 - sd = 761.556ratioG_corr - Cross - Nuc : ave = 0.180 - sd = 0.104r = 0.665 - r^2 = 0.442
c_corr [nM] - Ch1 - Cyto : ave = 250.554 - sd = 505.899Int_corr [kHz] - Ch1 - Cyto : ave = 11.703 - sd = 18.267r = 0.921 - r^2 = 0.848
f_1 - Ch1 - Cyto : ave = 0.846 - sd = 0.194D_1 [mum^2/s] - Ch1 - Cyto : ave = 23.671 - sd = 22.863r = 0.355 - r^2 = 0.126
f_2 - Ch1 - Cyto : ave = 0.154 - sd = 0.194D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.608 - sd = 0.357r = -0.398 - r^2 = 0.159
CPM_corr [kHz] - Ch1 - Cyto : ave = 8.811 - sd = 43.737D_m [mum^2/s] - Ch1 - Cyto : ave = 22.163 - sd = 22.688r = 0.766 - r^2 = 0.587