Automated F(C)CS analysis of clone # 3155

Created on 12/08/2014
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Gene and protein information

Clone # 3155
Gene symbol: GATA3
Description: GATA binding protein 3
Molecular weight (with FP): 74.9 kDa
Localisation: granular nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
3155_img_ch1.png3155_img_ch2.png


Point 1, intensity traces and correlation functions
3155_itr_1.png3155_cor_1.png

Point 2, intensity traces and correlation functions
3155_itr_2.png3155_cor_2.png

Point 3, intensity traces and correlation functions
3155_itr_3.png3155_cor_3.png

Point 4, intensity traces and correlation functions
3155_itr_4.png3155_cor_4.png

Point 5, intensity traces and correlation functions
3155_itr_5.png3155_cor_5.png

Point 6, intensity traces and correlation functions
3155_itr_6.png3155_cor_6.png

Sessions

1 20120203\U01--V00--3155
2 20120228\U00--V00--3155

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.303E+2 6.691E+1 1.036E+3 4.640E+2 1.505E+1 1.139E+1 3.696E+1 5.617E+1 3.695E+2 2.893E+2 9.091E+1 3.177E+1
N_corr 1.638E+2 8.994E+1 1.602E+3 8.675E+2 2.336E+1 1.645E+1 3.959E+1 6.995E+1 5.589E+2 5.470E+2 1.689E+2 6.904E+1
c [nM] 8.107E+2 4.161E+2 4.804E+3 2.152E+3 7.735E+1 5.858E+1 2.299E+2 3.493E+2 1.714E+3 1.342E+3 4.674E+2 1.633E+2
c_corr [nM] 1.019E+3 5.594E+2 7.432E+3 4.024E+3 1.201E+2 8.459E+1 2.462E+2 4.351E+2 2.593E+3 2.537E+3 8.681E+2 3.549E+2
ratioG 1.002E-1 4.943E-2 4.984E-1 1.755E-2
ratioG_corr 4.337E-2 4.714E-2 5.725E-1 1.698E-2
f_1 5.755E-1 1.363E-1 3.964E-1 2.044E-1 8.397E-1 1.662E-1 8.907E-1 8.667E-2 7.062E-1 3.500E-1 9.391E-1 4.995E-2
tauD_1 [ms] 3.142E+0 3.002E+0 1.860E+0 2.220E-16 7.358E+0 1.089E+1 8.143E-1 6.639E-1 1.860E+0 2.220E-16 2.814E+0 2.990E-1
D_1 [mum^2/s] 6.771E+0 5.577E+0 6.926E+0 8.882E-16 1.644E+1 3.240E+1 2.019E+1 1.432E+1 6.926E+0 0.000E+0 4.309E+0 4.482E-1
alpha_1 9.210E-1 1.051E-1 9.200E-1 2.220E-16 7.470E-1 2.034E-1 9.677E-1 1.372E-1 9.200E-1 1.110E-16 1.200E+0 0.000E+0
f_2 4.245E-1 1.363E-1 6.036E-1 2.044E-1 1.603E-1 1.662E-1 1.093E-1 8.667E-2 2.938E-1 3.500E-1 6.094E-2 4.995E-2
tauD_2 [ms] 5.063E+1 3.686E+1 1.097E+2 1.231E+2 3.173E+2 7.743E+2 2.327E+1 1.824E+1 6.674E+1 7.170E+1 7.173E+1 1.155E+1
D_2 [mum^2/s] 2.918E-1 1.403E-1 3.759E-1 5.480E-1 9.863E-2 8.885E-2 6.590E-1 3.468E-1 1.137E+0 1.641E+0 1.714E-1 2.674E-2
alpha_2 1.550E+0 2.601E-1 1.303E+0 3.019E-1 1.308E+0 3.478E-1 1.118E+0 1.614E-1 1.406E+0 3.996E-1 1.121E+0 5.288E-1
tauD_m [ms] 2.089E+1 1.137E+1 6.575E+1 7.324E+1 5.923E+1 1.259E+2 3.657E+0 4.388E+0 1.451E+1 2.431E+1 7.579E+0 4.327E+0
D_m [mum^2/s] 3.531E+0 2.367E+0 2.994E+0 1.311E+0 1.280E+1 2.611E+1 1.826E+1 1.390E+1 5.709E+0 1.227E+0 4.065E+0 5.600E-1
R [1/s] 1.111E+1 1.112E+1 6.050E+0 3.264E+0
CPM [kHz] 9.303E-1 2.486E-1 4.253E-1 3.673E-1 4.639E-1 3.037E-1 2.317E-1 1.257E-1
CPM_corr [kHz] 9.477E-1 2.516E-1 4.811E-1 4.149E-1 7.203E-1 4.922E-1 7.081E-1 1.734E+0
Int [kHz] 1.173E+2 5.062E+1 1.721E+2 4.710E+1 1.296E+1 2.138E+1 6.462E+1 4.155E+1
Int_corr [kHz] 1.495E+2 6.876E+1 2.939E+2 1.031E+2 1.677E+1 3.106E+1 1.090E+2 8.612E+1
# samples 244 244 165 165 39 39 176 176 18 18 3 3
# cells 88

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 1018.585 - sd = 559.369Int_corr [kHz] - Ch1 - Nuc : ave = 149.540 - sd = 68.759r = 0.874 - r^2 = 0.764
f_1 - Ch1 - Nuc : ave = 0.575 - sd = 0.136D_1 [mum^2/s] - Ch1 - Nuc : ave = 6.771 - sd = 5.577r = -0.668 - r^2 = 0.446
f_2 - Ch1 - Nuc : ave = 0.425 - sd = 0.136D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.292 - sd = 0.140r = 0.376 - r^2 = 0.141
CPM_corr [kHz] - Ch1 - Nuc : ave = 0.948 - sd = 0.252D_m [mum^2/s] - Ch1 - Nuc : ave = 3.531 - sd = 2.367r = 0.125 - r^2 = 0.016
c_corr [nM] - Ch1 - Nuc : ave = 1018.585 - sd = 559.369ratioG_corr - Cross - Nuc : ave = 0.043 - sd = 0.047r = 0.248 - r^2 = 0.062
c_corr [nM] - Ch1 - Cyto : ave = 246.190 - sd = 435.065Int_corr [kHz] - Ch1 - Cyto : ave = 16.772 - sd = 31.057r = 0.781 - r^2 = 0.609
f_1 - Ch1 - Cyto : ave = 0.891 - sd = 0.087D_1 [mum^2/s] - Ch1 - Cyto : ave = 20.187 - sd = 14.324r = 0.006 - r^2 = 4.071E-5
f_2 - Ch1 - Cyto : ave = 0.109 - sd = 0.087D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.659 - sd = 0.347r = -0.137 - r^2 = 0.019
CPM_corr [kHz] - Ch1 - Cyto : ave = 0.720 - sd = 0.492D_m [mum^2/s] - Ch1 - Cyto : ave = 18.262 - sd = 13.897r = 0.215 - r^2 = 0.046