Automated F(C)CS analysis of clone # 3140

Created on 12/08/2014
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Gene and protein information

Clone # 3140
Gene symbol: XBP1
Description: X-box binding protein 1
Molecular weight (with FP): 55.7 kDa
Localisation: granular nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
3140_img_ch1.png3140_img_ch2.png


Point 1, intensity traces and correlation functions
3140_itr_1.png3140_cor_1.png

Point 2, intensity traces and correlation functions
3140_itr_2.png3140_cor_2.png

Point 3, intensity traces and correlation functions
3140_itr_3.png3140_cor_3.png

Point 4, intensity traces and correlation functions
3140_itr_4.png3140_cor_4.png

Point 5, intensity traces and correlation functions
3140_itr_5.png3140_cor_5.png

Point 6, intensity traces and correlation functions
3140_itr_6.png3140_cor_6.png

Sessions

1 20110913\U02--V00--3140
2 20110914\U02--V01--3140
3 20110923\U03--V00--3140
4 20111011\U03--V01--3140

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 3.401E+1 2.505E+1 8.198E+2 3.311E+2 2.493E+0 4.115E+0 1.986E+1 1.149E+1 1.526E+2 1.847E+2 8.568E+0 7.266E+0
N_corr 3.862E+1 3.313E+1 1.223E+3 6.060E+2 3.525E+0 6.789E+0 2.344E+1 1.730E+1 1.783E+2 2.970E+2 1.160E+1 9.714E+0
c [nM] 2.115E+2 1.558E+2 3.803E+3 1.536E+3 1.282E+1 2.115E+1 1.235E+2 7.147E+1 7.077E+2 8.566E+2 4.405E+1 3.735E+1
c_corr [nM] 2.402E+2 2.061E+2 5.673E+3 2.811E+3 1.812E+1 3.490E+1 1.458E+2 1.076E+2 8.271E+2 1.377E+3 5.961E+1 4.994E+1
ratioG 4.912E-2 3.272E-2 4.004E-1 2.449E-1
ratioG_corr 9.382E-3 2.001E-2 8.559E-2 7.853E-2
f_1 8.214E-1 1.016E-1 4.697E-1 2.235E-1 9.633E-1 9.614E-2 8.345E-1 1.150E-1 9.669E-1 1.127E-1 9.433E-1 7.118E-2
tauD_1 [ms] 1.373E+0 1.088E+0 1.877E+0 3.116E-2 1.479E+0 1.493E+0 1.005E+0 1.404E+0 4.564E+0 1.057E+1 7.763E-1 9.512E-1
D_1 [mum^2/s] 1.478E+1 1.225E+1 6.865E+0 1.165E-1 2.422E+1 2.982E+1 2.131E+1 1.503E+1 1.964E+1 2.837E+1 4.453E+1 3.517E+1
alpha_1 9.838E-1 1.289E-1 9.658E-1 3.958E-2 8.572E-1 2.330E-1 9.583E-1 1.880E-1 8.564E-1 2.421E-1 8.294E-1 2.227E-1
f_2 1.786E-1 1.016E-1 5.303E-1 2.235E-1 3.667E-2 9.614E-2 1.655E-1 1.150E-1 3.311E-2 1.127E-1 5.671E-2 7.118E-2
tauD_2 [ms] 2.414E+1 1.543E+1 1.023E+2 1.302E+2 9.488E+1 7.644E+1 2.902E+1 2.728E+1 2.221E+1 5.495E+1 4.092E+1 3.519E+1
D_2 [mum^2/s] 6.719E-1 4.802E-1 5.906E-1 8.965E-1 1.826E-1 1.022E-1 6.204E-1 4.465E-1 2.171E+0 8.913E-1 5.359E-1 3.908E-1
alpha_2 1.420E+0 3.457E-1 1.380E+0 3.556E-1 1.159E+0 2.280E-1 1.217E+0 2.976E-1 1.133E+0 1.472E-1 1.166E+0 2.183E-1
tauD_m [ms] 5.983E+0 4.864E+0 5.405E+1 8.056E+1 7.059E+0 2.145E+1 6.705E+0 8.642E+0 7.955E+0 1.693E+1 3.102E+0 5.601E+0
D_m [mum^2/s] 1.273E+1 1.147E+1 3.639E+0 1.207E+0 2.362E+1 2.965E+1 1.860E+1 1.431E+1 1.942E+1 2.848E+1 4.231E+1 3.402E+1
R [1/s] 4.844E+1 5.529E+1 3.423E+0 9.646E+0
CPM [kHz] 1.551E+0 4.596E-1 3.335E-1 3.353E-1 1.410E+0 4.806E-1 2.098E-1 1.726E-1
CPM_corr [kHz] 1.627E+0 4.631E-1 3.618E-1 3.651E-1 1.787E+0 3.254E+0 7.643E-1 7.599E-1
Int [kHz] 5.773E+1 4.572E+1 1.451E+2 4.234E+1 3.100E+1 1.981E+1 3.138E+1 4.263E+1
Int_corr [kHz] 6.700E+1 5.685E+1 2.275E+2 7.814E+1 3.783E+1 2.393E+1 4.673E+1 6.609E+1
# samples 197 197 145 145 124 124 309 309 89 89 81 81
# cells 107

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 240.169 - sd = 206.072Int_corr [kHz] - Ch1 - Nuc : ave = 67.000 - sd = 56.846r = 0.635 - r^2 = 0.403
f_1 - Ch1 - Nuc : ave = 0.821 - sd = 0.102D_1 [mum^2/s] - Ch1 - Nuc : ave = 14.776 - sd = 12.245r = 0.256 - r^2 = 0.066
f_2 - Ch1 - Nuc : ave = 0.179 - sd = 0.102D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.672 - sd = 0.480r = -0.430 - r^2 = 0.184
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.627 - sd = 0.463D_m [mum^2/s] - Ch1 - Nuc : ave = 12.729 - sd = 11.470r = -0.189 - r^2 = 0.036
c_corr [nM] - Ch1 - Nuc : ave = 240.169 - sd = 206.072ratioG_corr - Cross - Nuc : ave = 0.009 - sd = 0.020r = 0.211 - r^2 = 0.044
c_corr [nM] - Ch1 - Cyto : ave = 145.756 - sd = 107.602Int_corr [kHz] - Ch1 - Cyto : ave = 37.826 - sd = 23.927r = 0.793 - r^2 = 0.629
f_1 - Ch1 - Cyto : ave = 0.834 - sd = 0.115D_1 [mum^2/s] - Ch1 - Cyto : ave = 21.310 - sd = 15.027r = 0.409 - r^2 = 0.167
f_2 - Ch1 - Cyto : ave = 0.166 - sd = 0.115D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.620 - sd = 0.447r = -0.532 - r^2 = 0.283
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.787 - sd = 3.254D_m [mum^2/s] - Ch1 - Cyto : ave = 18.595 - sd = 14.313r = -0.109 - r^2 = 0.012