Automated F(C)CS analysis of clone # 3099

Created on 12/08/2014
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Gene and protein information

Clone # 3099
Gene symbol: SF3B5
Description: splicing factor 3b, subunit 5, 10kDa
Molecular weight (with FP): 37.1 kDa
Localisation: granular nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
3099_img_ch1.png3099_img_ch2.png


Point 1, intensity traces and correlation functions
3099_itr_1.png3099_cor_1.png

Point 2, intensity traces and correlation functions
3099_itr_2.png3099_cor_2.png

Point 3, intensity traces and correlation functions
3099_itr_3.png3099_cor_3.png

Point 4, intensity traces and correlation functions
3099_itr_4.png3099_cor_4.png

Point 5, intensity traces and correlation functions
3099_itr_5.png3099_cor_5.png

Point 6, intensity traces and correlation functions
3099_itr_6.png3099_cor_6.png

Sessions

1 20110826\U02--V00--3099
2 20110826\U03--V00--3099
3 20110909\U03--V00--3099
4 20110923\U01--V00--3099
5 20110927\U01--V00--3099
6 20110928\U01--V00--3099
7 20110930\U01--V00--3099

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 7.274E+1 5.079E+1 9.312E+2 3.441E+2 7.715E+0 1.059E+1 4.901E+1 6.004E+1 2.017E+2 3.063E+2 3.026E+1 3.871E+1
N_corr 7.816E+1 5.485E+1 1.364E+3 5.659E+2 8.745E+0 1.196E+1 5.556E+1 7.241E+1 1.904E+2 3.655E+2 3.691E+1 5.200E+1
c [nM] 4.524E+2 3.159E+2 4.319E+3 1.596E+3 3.966E+1 5.444E+1 3.048E+2 3.734E+2 9.356E+2 1.421E+3 1.555E+2 1.990E+2
c_corr [nM] 4.861E+2 3.411E+2 6.326E+3 2.625E+3 4.496E+1 6.147E+1 3.455E+2 4.503E+2 8.833E+2 1.695E+3 1.898E+2 2.673E+2
ratioG 7.925E-2 5.983E-2 5.408E-1 2.302E-1
ratioG_corr 9.766E-3 4.278E-2 1.075E-1 1.094E-1
f_1 8.641E-1 7.159E-2 4.647E-1 2.195E-1 9.927E-1 3.105E-2 9.035E-1 5.950E-2 9.850E-1 7.978E-2 9.817E-1 4.634E-2
tauD_1 [ms] 1.087E+0 5.720E-1 1.815E+0 1.124E-1 8.255E-1 8.599E-1 7.797E-1 4.755E-1 1.845E+0 2.918E+0 4.609E-1 3.137E-1
D_1 [mum^2/s] 1.186E+1 4.682E+0 7.127E+0 4.478E-1 3.219E+1 3.125E+1 1.638E+1 7.315E+0 2.108E+1 2.426E+1 3.401E+1 1.676E+1
alpha_1 9.740E-1 8.634E-2 9.212E-1 1.809E-2 8.508E-1 2.204E-1 1.022E+0 1.501E-1 8.931E-1 2.211E-1 7.956E-1 1.657E-1
f_2 1.359E-1 7.159E-2 5.353E-1 2.195E-1 7.337E-3 3.105E-2 9.654E-2 5.950E-2 1.501E-2 7.978E-2 1.828E-2 4.634E-2
tauD_2 [ms] 1.936E+1 9.702E+0 8.090E+1 1.148E+2 7.118E+1 5.151E+1 1.949E+1 2.025E+1 2.546E+1 6.081E+1 2.205E+1 2.688E+1
D_2 [mum^2/s] 6.643E-1 2.763E-1 5.811E-1 8.535E-1 2.445E-1 1.506E-1 8.016E-1 3.571E-1 1.581E+0 1.062E+0 8.248E-1 5.167E-1
alpha_2 1.347E+0 3.045E-1 1.336E+0 3.298E-1 1.134E+0 6.571E-2 1.135E+0 1.977E-1 1.039E+0 7.969E-2 1.159E+0 1.352E-1
tauD_m [ms] 3.600E+0 2.291E+0 4.064E+1 5.141E+1 1.652E+0 4.568E+0 2.320E+0 2.413E+0 5.818E+0 2.594E+1 8.427E-1 2.068E+0
D_m [mum^2/s] 1.040E+1 4.300E+0 3.667E+0 1.398E+0 3.189E+1 3.078E+1 1.479E+1 6.095E+0 2.098E+1 2.433E+1 3.333E+1 1.650E+1
R [1/s] 4.331E+1 3.526E+1 2.573E+1 3.796E+1
CPM [kHz] 1.404E+0 2.810E-1 2.942E-1 2.162E-1 1.381E+0 4.853E-1 1.684E-1 1.215E-1
CPM_corr [kHz] 1.442E+0 2.848E-1 3.329E-1 2.501E-1 1.473E+0 4.742E-1 6.400E-1 3.880E-1
Int [kHz] 1.120E+2 7.853E+1 1.495E+2 3.971E+1 7.408E+1 1.450E+2 3.351E+1 4.513E+1
Int_corr [kHz] 1.219E+2 8.353E+1 2.436E+2 6.952E+1 8.195E+1 1.510E+2 5.289E+1 8.598E+1
# samples 236 236 168 168 149 149 317 317 115 115 104 104
# cells 136

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 486.091 - sd = 341.130Int_corr [kHz] - Ch1 - Nuc : ave = 121.864 - sd = 83.529r = 0.922 - r^2 = 0.850
f_1 - Ch1 - Nuc : ave = 0.864 - sd = 0.072D_1 [mum^2/s] - Ch1 - Nuc : ave = 11.858 - sd = 4.682r = 0.444 - r^2 = 0.197
f_2 - Ch1 - Nuc : ave = 0.136 - sd = 0.072D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.664 - sd = 0.276r = -0.465 - r^2 = 0.216
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.442 - sd = 0.285D_m [mum^2/s] - Ch1 - Nuc : ave = 10.396 - sd = 4.300r = 0.242 - r^2 = 0.059
c_corr [nM] - Ch1 - Nuc : ave = 486.091 - sd = 341.130ratioG_corr - Cross - Nuc : ave = 0.010 - sd = 0.043r = 0.328 - r^2 = 0.108
c_corr [nM] - Ch1 - Cyto : ave = 345.520 - sd = 450.324Int_corr [kHz] - Ch1 - Cyto : ave = 81.949 - sd = 150.984r = 0.868 - r^2 = 0.753
f_1 - Ch1 - Cyto : ave = 0.903 - sd = 0.060D_1 [mum^2/s] - Ch1 - Cyto : ave = 16.384 - sd = 7.315r = -0.133 - r^2 = 0.018
f_2 - Ch1 - Cyto : ave = 0.097 - sd = 0.060D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.802 - sd = 0.357r = 0.195 - r^2 = 0.038
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.473 - sd = 0.474D_m [mum^2/s] - Ch1 - Cyto : ave = 14.788 - sd = 6.095r = 0.296 - r^2 = 0.088