Automated F(C)CS analysis of clone # 3033

Created on 12/08/2014
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Gene and protein information

Clone # 3033
Gene symbol: SENP3
Description: SUMO1/sentrin/SMT3 specific peptidase 3
Molecular weight (with FP): 91.9 kDa
Localisation: granular nucleus, bright nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
3033_img_ch1.png3033_img_ch2.png


Point 1, intensity traces and correlation functions
3033_itr_1.png3033_cor_1.png

Point 2, intensity traces and correlation functions
3033_itr_2.png3033_cor_2.png

Point 3, intensity traces and correlation functions
3033_itr_3.png3033_cor_3.png

Point 4, intensity traces and correlation functions
3033_itr_4.png3033_cor_4.png

Point 5, intensity traces and correlation functions
3033_itr_5.png3033_cor_5.png

Point 6, intensity traces and correlation functions
3033_itr_6.png3033_cor_6.png

Sessions

1 20111123\U03--V00--3033
2 20111207\U03--V00--3033
3 20120125\U01--V00--3033
4 20120223\U02--V00--3033

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 2.076E+2 2.042E+2 1.138E+3 5.963E+2 1.936E+1 2.457E+1 1.903E+1 1.622E+1 2.234E+2 1.991E+2 6.804E+0 6.883E+0
N_corr 2.771E+2 3.070E+2 1.563E+3 9.741E+2 2.868E+1 3.947E+1 1.933E+1 1.902E+1 3.043E+2 3.262E+2 1.240E+1 1.734E+1
c [nM] 1.291E+3 1.270E+3 5.278E+3 2.766E+3 9.953E+1 1.263E+2 1.183E+2 1.009E+2 1.036E+3 9.235E+2 3.498E+1 3.539E+1
c_corr [nM] 1.724E+3 1.910E+3 7.248E+3 4.518E+3 1.475E+2 2.029E+2 1.202E+2 1.183E+2 1.412E+3 1.513E+3 6.376E+1 8.916E+1
ratioG 1.061E-1 8.255E-2 2.809E-1 2.298E-1
ratioG_corr 7.842E-3 8.219E-2 9.306E-2 8.482E-2
f_1 7.612E-1 1.453E-1 4.008E-1 2.483E-1 9.413E-1 1.253E-1 8.865E-1 6.000E-2 6.507E-1 4.300E-1 9.323E-1 9.401E-2
tauD_1 [ms] 2.979E+0 2.120E+0 1.860E+0 4.441E-16 2.570E+0 3.952E+0 6.889E-1 4.427E-1 1.860E+0 0.000E+0 1.215E+0 8.794E-1
D_1 [mum^2/s] 4.574E+0 2.394E+0 6.926E+0 8.882E-16 1.352E+1 1.885E+1 2.243E+1 1.526E+1 6.926E+0 0.000E+0 2.675E+1 3.289E+1
alpha_1 9.782E-1 1.199E-1 9.200E-1 0.000E+0 8.184E-1 2.429E-1 9.776E-1 1.084E-1 9.200E-1 0.000E+0 9.521E-1 2.477E-1
f_2 2.388E-1 1.453E-1 5.992E-1 2.483E-1 5.874E-2 1.253E-1 1.135E-1 6.000E-2 3.493E-1 4.300E-1 6.768E-2 9.401E-2
tauD_2 [ms] 5.009E+1 4.208E+1 6.918E+1 9.947E+1 1.144E+2 1.078E+2 1.819E+1 1.876E+1 7.218E+1 1.034E+2 5.628E+1 7.622E+1
D_2 [mum^2/s] 3.104E-1 1.597E-1 8.668E-1 1.042E+0 1.611E-1 8.440E-2 8.310E-1 4.010E-1 1.082E+0 1.395E+0 5.126E-1 4.232E-1
alpha_2 1.526E+0 3.283E-1 1.289E+0 3.256E-1 1.190E+0 2.858E-1 1.142E+0 1.820E-1 1.209E+0 3.395E-1 1.248E+0 3.243E-1
tauD_m [ms] 1.436E+1 1.220E+1 3.815E+1 6.588E+1 1.453E+1 3.679E+1 2.972E+0 3.776E+0 2.155E+1 5.433E+1 4.212E+0 6.508E+0
D_m [mum^2/s] 3.440E+0 1.871E+0 3.410E+0 1.381E+0 1.273E+1 1.833E+1 2.025E+1 1.476E+1 5.261E+0 2.155E+0 2.537E+1 3.234E+1
R [1/s] 5.663E+0 8.704E+0 4.069E+1 3.019E+1
CPM [kHz] 1.518E+0 5.107E-1 3.136E-1 2.983E-1 9.326E-1 4.400E-1 2.768E-1 3.412E-1
CPM_corr [kHz] 1.536E+0 5.175E-1 3.710E-1 3.405E-1 1.124E+0 4.815E-1 -2.807E-1 3.541E+0
Int [kHz] 2.561E+2 1.952E+2 1.833E+2 4.535E+1 1.850E+1 1.701E+1 4.333E+1 4.457E+1
Int_corr [kHz] 3.360E+2 2.943E+2 2.896E+2 8.797E+1 2.121E+1 2.054E+1 7.240E+1 8.468E+1
# samples 184 184 120 120 107 107 144 144 16 16 13 13
# cells 64

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 1723.566 - sd = 1909.566Int_corr [kHz] - Ch1 - Nuc : ave = 336.045 - sd = 294.286r = 0.860 - r^2 = 0.739
f_1 - Ch1 - Nuc : ave = 0.761 - sd = 0.145D_1 [mum^2/s] - Ch1 - Nuc : ave = 4.574 - sd = 2.394r = -0.495 - r^2 = 0.245
f_2 - Ch1 - Nuc : ave = 0.239 - sd = 0.145D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.310 - sd = 0.160r = -0.033 - r^2 = 0.001
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.536 - sd = 0.518D_m [mum^2/s] - Ch1 - Nuc : ave = 3.440 - sd = 1.871r = -0.282 - r^2 = 0.080
c_corr [nM] - Ch1 - Nuc : ave = 1723.566 - sd = 1909.566ratioG_corr - Cross - Nuc : ave = 0.008 - sd = 0.082r = 0.357 - r^2 = 0.128
c_corr [nM] - Ch1 - Cyto : ave = 120.203 - sd = 118.276Int_corr [kHz] - Ch1 - Cyto : ave = 21.213 - sd = 20.542r = 0.594 - r^2 = 0.352
f_1 - Ch1 - Cyto : ave = 0.886 - sd = 0.060D_1 [mum^2/s] - Ch1 - Cyto : ave = 22.430 - sd = 15.259r = 0.265 - r^2 = 0.070
f_2 - Ch1 - Cyto : ave = 0.114 - sd = 0.060D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.831 - sd = 0.401r = -0.404 - r^2 = 0.163
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.124 - sd = 0.481D_m [mum^2/s] - Ch1 - Cyto : ave = 20.249 - sd = 14.760r = -0.138 - r^2 = 0.019