Automated F(C)CS analysis of clone # 2960

Created on 12/08/2014
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Gene and protein information

Clone # 2960
Gene symbol: F8A2
Description: coagulation factor VIII-associated 2
Molecular weight (with FP): 66.1 kDa
Localisation: homogeneous nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
2960_img_ch1.png2960_img_ch2.png


Point 1, intensity traces and correlation functions
2960_itr_1.png2960_cor_1.png

Point 2, intensity traces and correlation functions
2960_itr_2.png2960_cor_2.png

Point 3, intensity traces and correlation functions
2960_itr_3.png2960_cor_3.png

Point 4, intensity traces and correlation functions
2960_itr_4.png2960_cor_4.png

Point 5, intensity traces and correlation functions
2960_itr_5.png2960_cor_5.png

Point 6, intensity traces and correlation functions
2960_itr_6.png2960_cor_6.png

Sessions

1 20110914\U01--V01--2960
2 20110920\U00--V01--2960
3 20110922\U03--V01--2960
4 20111011\U01--V01--2960
5 20111123\U02--V00--2960
6 20120116\U03--V00--2960
7 20120222\U00--V00--2960

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.010E+2 5.328E+1 1.120E+3 5.315E+2 9.721E+0 8.135E+0 4.980E+1 2.980E+1 8.438E+1 5.749E+1 2.571E+1 1.130E+1
N_corr 1.058E+2 6.225E+1 1.647E+3 1.001E+3 1.049E+1 8.819E+0 5.392E+1 3.326E+1 4.208E+1 1.009E+2 3.717E+1 1.987E+1
c [nM] 6.280E+2 3.314E+2 5.193E+3 2.465E+3 4.997E+1 4.182E+1 3.097E+2 1.853E+2 3.914E+2 2.666E+2 1.322E+2 5.811E+1
c_corr [nM] 6.580E+2 3.872E+2 7.639E+3 4.645E+3 5.392E+1 4.534E+1 3.353E+2 2.069E+2 1.952E+2 4.681E+2 1.911E+2 1.022E+2
ratioG 8.447E-2 4.400E-2 6.177E-1 1.228E-1
ratioG_corr 7.597E-3 2.325E-2 1.415E-1 9.878E-2
f_1 8.997E-1 5.955E-2 4.110E-1 2.309E-1 9.883E-1 3.767E-2 8.981E-1 7.275E-2 8.852E-1 1.594E-1 9.712E-1 8.721E-2
tauD_1 [ms] 1.425E+0 7.168E-1 1.842E+0 2.733E-2 9.405E-1 8.849E-1 1.049E+0 6.808E-1 1.826E+0 5.485E-2 6.387E-1 6.751E-1
D_1 [mum^2/s] 9.093E+0 4.035E+0 6.994E+0 1.051E-1 2.831E+1 2.986E+1 1.309E+1 5.856E+0 7.064E+0 2.357E-1 2.556E+1 1.068E+1
alpha_1 9.574E-1 7.530E-2 9.200E-1 0.000E+0 8.999E-1 2.133E-1 9.316E-1 1.093E-1 9.092E-1 5.921E-2 7.156E-1 7.881E-2
f_2 1.003E-1 5.955E-2 5.890E-1 2.309E-1 1.173E-2 3.767E-2 1.019E-1 7.275E-2 1.148E-1 1.594E-1 2.885E-2 8.721E-2
tauD_2 [ms] 1.885E+1 7.998E+0 6.641E+1 6.959E+1 7.029E+1 4.209E+1 2.871E+1 5.097E+1 9.793E+1 2.855E+2 4.094E+1 9.335E+1
D_2 [mum^2/s] 6.408E-1 2.105E-1 5.887E-1 7.709E-1 2.289E-1 1.271E-1 5.616E-1 2.539E-1 9.815E-1 2.081E+0 5.889E-1 2.435E-1
alpha_2 1.369E+0 3.222E-1 1.299E+0 3.225E-1 1.084E+0 1.017E-1 1.177E+0 2.563E-1 1.085E+0 1.046E-1 1.142E+0 2.137E-1
tauD_m [ms] 3.188E+0 1.914E+0 3.816E+1 4.704E+1 2.190E+0 4.844E+0 5.202E+0 1.861E+1 2.965E+1 1.260E+2 6.107E+0 2.393E+1
D_m [mum^2/s] 8.207E+0 3.539E+0 3.309E+0 1.318E+0 2.810E+1 2.983E+1 1.168E+1 4.991E+0 6.420E+0 1.147E+0 2.515E+1 1.081E+1
R [1/s] 4.466E+1 3.244E+1 1.951E+1 2.600E+1
CPM [kHz] 1.428E+0 2.905E-1 3.578E-1 3.193E-1 1.253E+0 5.437E-1 1.562E-1 8.935E-2
CPM_corr [kHz] 1.452E+0 2.937E-1 4.064E-1 3.546E-1 1.311E+0 5.552E-1 8.216E-1 3.688E-1
Int [kHz] 1.550E+2 7.582E+1 2.007E+2 7.025E+1 6.056E+1 3.419E+1 1.322E+1 7.957E+0
Int_corr [kHz] 1.621E+2 7.947E+1 3.257E+2 1.313E+2 6.717E+1 3.712E+1 2.077E+1 3.555E+1
# samples 145 145 126 126 109 109 118 118 31 31 31 31
# cells 53

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 658.004 - sd = 387.159Int_corr [kHz] - Ch1 - Nuc : ave = 162.113 - sd = 79.473r = 0.882 - r^2 = 0.777
f_1 - Ch1 - Nuc : ave = 0.900 - sd = 0.060D_1 [mum^2/s] - Ch1 - Nuc : ave = 9.093 - sd = 4.035r = -0.232 - r^2 = 0.054
f_2 - Ch1 - Nuc : ave = 0.100 - sd = 0.060D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.641 - sd = 0.210r = 0.019 - r^2 = 0.000
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.452 - sd = 0.294D_m [mum^2/s] - Ch1 - Nuc : ave = 8.207 - sd = 3.539r = 0.102 - r^2 = 0.010
c_corr [nM] - Ch1 - Nuc : ave = 658.004 - sd = 387.159ratioG_corr - Cross - Nuc : ave = 0.008 - sd = 0.023r = 0.226 - r^2 = 0.051
c_corr [nM] - Ch1 - Cyto : ave = 335.335 - sd = 206.856Int_corr [kHz] - Ch1 - Cyto : ave = 67.173 - sd = 37.117r = 0.355 - r^2 = 0.126
f_1 - Ch1 - Cyto : ave = 0.898 - sd = 0.073D_1 [mum^2/s] - Ch1 - Cyto : ave = 13.088 - sd = 5.856r = -0.466 - r^2 = 0.217
f_2 - Ch1 - Cyto : ave = 0.102 - sd = 0.073D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.562 - sd = 0.254r = 0.004 - r^2 = 1.627E-5
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.311 - sd = 0.555D_m [mum^2/s] - Ch1 - Cyto : ave = 11.683 - sd = 4.991r = 0.336 - r^2 = 0.113