Automated F(C)CS analysis of clone # 2692

Created on 12/08/2014
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Gene and protein information

Clone # 2692
Gene symbol: H3F3B
Description: H3 histone, family 3B (H3.3B)
Molecular weight (with FP): 42.3 kDa
Localisation: granular nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
2692_img_ch1.png2692_img_ch2.png


Point 1, intensity traces and correlation functions
2692_itr_1.png2692_cor_1.png

Point 2, intensity traces and correlation functions
2692_itr_2.png2692_cor_2.png

Point 3, intensity traces and correlation functions
2692_itr_3.png2692_cor_3.png

Point 4, intensity traces and correlation functions
2692_itr_4.png2692_cor_4.png

Point 5, intensity traces and correlation functions
2692_itr_5.png2692_cor_5.png

Point 6, intensity traces and correlation functions
2692_itr_6.png2692_cor_6.png

Sessions

1 20110913\U03--V00--2692
2 20110914\U00--V01--2692
3 20110921\U03--V00--2692
4 20110923\U00--V01--2692
5 20110928\U00--V01--2692

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 2.021E+2 1.781E+2 1.042E+3 5.384E+2 5.506E+1 6.132E+1 3.179E+1 6.055E+1 2.786E+2 3.217E+2 4.359E+1 7.079E+1
N_corr 2.436E+2 2.196E+2 1.227E+3 7.056E+2 8.127E+1 1.143E+2 3.930E+1 8.846E+1 3.341E+2 3.535E+2 8.658E+1 1.577E+2
c [nM] 1.257E+3 1.108E+3 4.834E+3 2.497E+3 2.831E+2 3.153E+2 1.977E+2 3.766E+2 1.292E+3 1.492E+3 2.241E+2 3.640E+2
c_corr [nM] 1.515E+3 1.366E+3 5.692E+3 3.273E+3 4.178E+2 5.874E+2 2.444E+2 5.502E+2 1.550E+3 1.640E+3 4.451E+2 8.106E+2
ratioG 1.969E-1 1.350E-1 4.208E-1 2.684E-1
ratioG_corr 5.511E-2 1.132E-1 2.766E-1 2.655E-1
f_1 8.826E-1 1.127E-1 4.978E-1 2.585E-1 9.639E-1 7.378E-2 9.410E-1 8.787E-2 9.659E-1 1.603E-1 8.840E-1 2.145E-1
tauD_1 [ms] 1.453E+0 8.190E-1 1.835E+0 4.562E-2 1.083E+0 8.037E-1 7.695E-1 3.361E+0 7.793E+0 1.510E+1 2.668E+0 8.687E+0
D_1 [mum^2/s] 9.582E+0 8.226E+0 7.025E+0 1.720E-1 2.034E+1 2.088E+1 2.753E+1 1.529E+1 1.514E+1 2.745E+1 2.783E+1 2.750E+1
alpha_1 9.599E-1 9.978E-2 9.200E-1 0.000E+0 8.704E-1 2.124E-1 9.762E-1 1.289E-1 9.539E-1 2.767E-1 9.778E-1 2.489E-1
f_2 1.174E-1 1.127E-1 5.022E-1 2.585E-1 3.607E-2 7.378E-2 5.896E-2 8.787E-2 3.406E-2 1.603E-1 1.160E-1 2.145E-1
tauD_2 [ms] 2.569E+1 1.825E+1 9.182E+1 1.160E+2 5.664E+1 4.634E+1 1.887E+1 3.414E+1 2.473E+1 8.962E+1 8.856E+1 1.199E+2
D_2 [mum^2/s] 5.595E-1 2.638E-1 6.829E-1 1.045E+0 3.155E-1 1.694E-1 1.044E+0 5.591E-1 2.321E+0 7.270E-1 4.669E-1 4.462E-1
alpha_2 1.268E+0 2.506E-1 1.271E+0 3.294E-1 1.129E+0 1.692E-1 1.107E+0 1.015E-1 1.073E+0 2.277E-1 1.197E+0 2.538E-1
tauD_m [ms] 5.142E+0 5.988E+0 4.584E+1 6.297E+1 3.770E+0 7.315E+0 3.194E+0 1.863E+1 1.548E+1 3.396E+1 2.963E+1 6.937E+1
D_m [mum^2/s] 8.727E+0 8.259E+0 3.964E+0 1.492E+0 1.963E+1 2.024E+1 2.626E+1 1.518E+1 1.489E+1 2.754E+1 2.524E+1 2.650E+1
R [1/s] 3.859E+1 3.494E+1 5.772E+0 2.778E+1
CPM [kHz] 1.476E+0 5.803E-1 3.401E-1 3.101E-1 8.117E-1 7.678E-1 3.648E-1 3.865E-1
CPM_corr [kHz] 1.500E+0 5.776E-1 4.493E-1 4.014E-1 1.098E+0 8.182E-1 6.435E-1 5.679E-1
Int [kHz] 3.155E+2 3.035E+2 1.695E+2 5.092E+1 2.687E+1 8.727E+1 6.461E+1 6.022E+1
Int_corr [kHz] 3.778E+2 3.766E+2 2.591E+2 8.728E+1 3.632E+1 1.236E+2 1.065E+2 1.056E+2
# samples 174 174 136 136 120 120 219 219 36 36 31 31
# cells 102

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 1515.195 - sd = 1365.818Int_corr [kHz] - Ch1 - Nuc : ave = 377.835 - sd = 376.622r = 0.874 - r^2 = 0.764
f_1 - Ch1 - Nuc : ave = 0.883 - sd = 0.113D_1 [mum^2/s] - Ch1 - Nuc : ave = 9.582 - sd = 8.226r = 0.343 - r^2 = 0.117
f_2 - Ch1 - Nuc : ave = 0.117 - sd = 0.113D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.559 - sd = 0.264r = -0.288 - r^2 = 0.083
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.500 - sd = 0.578D_m [mum^2/s] - Ch1 - Nuc : ave = 8.727 - sd = 8.259r = 0.380 - r^2 = 0.145
c_corr [nM] - Ch1 - Nuc : ave = 1515.195 - sd = 1365.818ratioG_corr - Cross - Nuc : ave = 0.055 - sd = 0.113r = 0.281 - r^2 = 0.079
c_corr [nM] - Ch1 - Cyto : ave = 244.401 - sd = 550.180Int_corr [kHz] - Ch1 - Cyto : ave = 36.324 - sd = 123.589r = 0.679 - r^2 = 0.461
f_1 - Ch1 - Cyto : ave = 0.941 - sd = 0.088D_1 [mum^2/s] - Ch1 - Cyto : ave = 27.533 - sd = 15.289r = -0.164 - r^2 = 0.027
f_2 - Ch1 - Cyto : ave = 0.059 - sd = 0.088D_2 [mum^2/s] - Ch1 - Cyto : ave = 1.044 - sd = 0.559r = 0.076 - r^2 = 0.006
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.098 - sd = 0.818D_m [mum^2/s] - Ch1 - Cyto : ave = 26.261 - sd = 15.175r = 0.376 - r^2 = 0.141