Automated F(C)CS analysis of clone # 2464

Created on 12/08/2014
Back to overview

Gene and protein information

Clone # 2464
Gene symbol: TCF12
Description: transcription factor 12
Molecular weight (with FP): 100.0 kDa
Localisation: granular nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
2464_img_ch1.png2464_img_ch2.png


Point 1, intensity traces and correlation functions
2464_itr_1.png2464_cor_1.png

Point 2, intensity traces and correlation functions
2464_itr_2.png2464_cor_2.png

Point 3, intensity traces and correlation functions
2464_itr_3.png2464_cor_3.png

Point 4, intensity traces and correlation functions
2464_itr_4.png2464_cor_4.png

Point 5, intensity traces and correlation functions
2464_itr_5.png2464_cor_5.png

Point 6, intensity traces and correlation functions
2464_itr_6.png2464_cor_6.png

Sessions

1 20111123\U00--V01--2464
2 20111125\U03--V00--2464
3 20111207\U02--V00--2464
4 20120217\U00--V00--2464

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.879E+2 1.007E+2 1.191E+3 5.520E+2 3.845E+1 4.057E+1 3.172E+1 5.475E+1 3.628E+2 2.463E+2 5.333E+1 6.530E+1
N_corr 2.055E+2 1.182E+2 1.460E+3 7.184E+2 5.131E+1 7.156E+1 3.107E+1 5.744E+1 4.746E+2 3.677E+2 6.344E+1 7.543E+1
c [nM] 1.169E+3 6.262E+2 5.525E+3 2.560E+3 1.977E+2 2.086E+2 1.973E+2 3.405E+2 1.683E+3 1.143E+3 2.741E+2 3.357E+2
c_corr [nM] 1.278E+3 7.350E+2 6.772E+3 3.332E+3 2.638E+2 3.679E+2 1.932E+2 3.573E+2 2.201E+3 1.705E+3 3.261E+2 3.878E+2
ratioG 1.660E-1 9.717E-2 3.158E-1 1.889E-1
ratioG_corr 3.001E-2 1.300E-1 2.452E-1 2.217E-1
f_1 8.701E-1 1.063E-1 3.847E-1 2.146E-1 9.593E-1 8.767E-2 9.125E-1 5.977E-2 5.632E-1 3.570E-1 9.158E-1 1.073E-1
tauD_1 [ms] 2.403E+0 1.164E+0 1.860E+0 2.220E-16 1.786E+0 1.116E+0 6.733E-1 6.607E-1 1.860E+0 0.000E+0 1.986E+0 8.838E-1
D_1 [mum^2/s] 5.081E+0 2.148E+0 6.926E+0 3.553E-15 1.118E+1 1.388E+1 2.596E+1 1.842E+1 6.926E+0 8.882E-16 1.154E+1 1.679E+1
alpha_1 9.513E-1 1.028E-1 9.200E-1 7.772E-16 8.476E-1 2.041E-1 1.009E+0 1.433E-1 9.200E-1 1.110E-16 1.120E+0 1.805E-1
f_2 1.299E-1 1.063E-1 6.153E-1 2.146E-1 4.073E-2 8.767E-2 8.746E-2 5.977E-2 4.368E-1 3.570E-1 8.418E-2 1.073E-1
tauD_2 [ms] 3.589E+1 2.520E+1 7.442E+1 9.109E+1 7.336E+1 6.306E+1 1.716E+1 2.231E+1 1.226E+2 1.930E+2 1.127E+2 1.597E+2
D_2 [mum^2/s] 3.767E-1 1.600E-1 5.477E-1 6.773E-1 2.401E-1 1.909E-1 9.105E-1 4.386E-1 1.161E+0 1.600E+0 2.392E-1 2.052E-1
alpha_2 1.343E+0 3.017E-1 1.255E+0 2.969E-1 1.159E+0 2.068E-1 1.106E+0 1.139E-1 1.389E+0 4.345E-1 1.113E+0 2.178E-1
tauD_m [ms] 7.418E+0 7.629E+0 4.329E+1 5.355E+1 5.705E+0 1.443E+1 2.472E+0 6.670E+0 4.755E+1 9.611E+1 2.519E+1 5.913E+1
D_m [mum^2/s] 4.424E+0 1.810E+0 3.051E+0 1.301E+0 1.074E+1 1.302E+1 2.395E+1 1.791E+1 4.842E+0 1.515E+0 1.049E+1 1.518E+1
R [1/s] 1.514E+1 1.812E+1 2.651E+1 5.763E+1
CPM [kHz] 1.345E+0 5.014E-1 3.010E-1 2.351E-1 5.846E-1 3.509E-1 2.958E-1 2.302E-1
CPM_corr [kHz] 1.359E+0 5.008E-1 3.865E-1 2.902E-1 7.987E-1 4.294E-1 7.896E-1 2.285E+0
Int [kHz] 2.760E+2 2.385E+2 1.867E+2 5.978E+1 1.501E+1 2.113E+1 6.914E+1 4.121E+1
Int_corr [kHz] 3.006E+2 2.624E+2 2.937E+2 1.066E+2 1.654E+1 2.286E+1 1.063E+2 6.543E+1
# samples 350 350 246 246 223 223 274 274 31 31 24 24
# cells 134

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 1277.973 - sd = 734.954Int_corr [kHz] - Ch1 - Nuc : ave = 300.562 - sd = 262.396r = 0.780 - r^2 = 0.609
f_1 - Ch1 - Nuc : ave = 0.870 - sd = 0.106D_1 [mum^2/s] - Ch1 - Nuc : ave = 5.081 - sd = 2.148r = -0.165 - r^2 = 0.027
f_2 - Ch1 - Nuc : ave = 0.130 - sd = 0.106D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.377 - sd = 0.160r = -0.066 - r^2 = 0.004
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.359 - sd = 0.501D_m [mum^2/s] - Ch1 - Nuc : ave = 4.424 - sd = 1.810r = -0.015 - r^2 = 0.000
c_corr [nM] - Ch1 - Nuc : ave = 1277.973 - sd = 734.954ratioG_corr - Cross - Nuc : ave = 0.030 - sd = 0.130r = 0.018 - r^2 = 0.000
c_corr [nM] - Ch1 - Cyto : ave = 193.244 - sd = 357.258Int_corr [kHz] - Ch1 - Cyto : ave = 16.545 - sd = 22.856r = 0.686 - r^2 = 0.470
f_1 - Ch1 - Cyto : ave = 0.913 - sd = 0.060D_1 [mum^2/s] - Ch1 - Cyto : ave = 25.955 - sd = 18.421r = -0.130 - r^2 = 0.017
f_2 - Ch1 - Cyto : ave = 0.087 - sd = 0.060D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.911 - sd = 0.439r = -0.106 - r^2 = 0.011
CPM_corr [kHz] - Ch1 - Cyto : ave = 0.799 - sd = 0.429D_m [mum^2/s] - Ch1 - Cyto : ave = 23.946 - sd = 17.909r = 0.291 - r^2 = 0.085