Automated F(C)CS analysis of clone # 2432

Created on 12/08/2014
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Gene and protein information

Clone # 2432
Gene symbol: CGGBP1
Description: CGG triplet repeat binding protein 1
Molecular weight (with FP): 45.8 kDa
Localisation: homogeneous nucleus, ave. nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
2432_img_ch1.png2432_img_ch2.png


Point 1, intensity traces and correlation functions
2432_itr_1.png2432_cor_1.png

Point 2, intensity traces and correlation functions
2432_itr_2.png2432_cor_2.png

Point 3, intensity traces and correlation functions
2432_itr_3.png2432_cor_3.png

Point 4, intensity traces and correlation functions
2432_itr_4.png2432_cor_4.png

Point 5, intensity traces and correlation functions
2432_itr_5.png2432_cor_5.png

Point 6, intensity traces and correlation functions
2432_itr_6.png2432_cor_6.png

Sessions

1 20110909\U02--V00--2432
2 20110923\U02--V00--2432
3 20110927\U02--V00--2432
4 20110928\U02--V00--2432

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.907E+2 2.025E+2 1.032E+3 3.679E+2 9.284E+1 1.266E+2 5.055E+1 5.914E+1 5.388E+2 3.406E+2 2.867E+1 3.926E+1
N_corr 2.100E+2 2.127E+2 1.099E+3 6.189E+2 1.308E+2 1.675E+2 4.422E+1 5.494E+1 6.620E+2 4.625E+2 2.648E+1 3.580E+1
c [nM] 1.186E+3 1.260E+3 4.788E+3 1.706E+3 4.773E+2 6.509E+2 3.144E+2 3.678E+2 2.499E+3 1.580E+3 1.474E+2 2.019E+2
c_corr [nM] 1.306E+3 1.323E+3 5.096E+3 2.871E+3 6.727E+2 8.609E+2 2.750E+2 3.417E+2 3.071E+3 2.145E+3 1.361E+2 1.841E+2
ratioG 2.376E-1 1.922E-1 2.236E-1 1.636E-1
ratioG_corr 4.743E-2 1.051E-1 1.393E-1 1.479E-1
f_1 8.891E-1 8.355E-2 5.558E-1 1.900E-1 9.193E-1 9.038E-2 9.676E-1 4.539E-2 7.285E-1 3.450E-1 9.432E-1 3.625E-2
tauD_1 [ms] 2.918E+0 1.459E+0 1.823E+0 2.919E-2 1.752E+0 9.985E-1 1.705E+0 7.624E-1 2.368E+0 9.249E-1 1.585E+0 5.949E-1
D_1 [mum^2/s] 4.333E+0 1.483E+0 7.068E+0 1.123E-1 9.863E+0 7.813E+0 8.815E+0 7.067E+0 6.033E+0 1.528E+0 8.796E+0 3.292E+0
alpha_1 1.068E+0 7.379E-2 9.200E-1 0.000E+0 8.233E-1 1.251E-1 1.009E+0 1.632E-1 1.032E+0 1.372E-1 1.073E+0 2.197E-1
f_2 1.109E-1 8.355E-2 4.442E-1 1.900E-1 8.067E-2 9.038E-2 3.240E-2 4.539E-2 2.715E-1 3.450E-1 5.676E-2 3.625E-2
tauD_2 [ms] 2.735E+1 1.239E+1 8.596E+1 5.462E+1 4.285E+1 1.743E+1 2.776E+1 9.730E+0 1.010E+2 1.329E+2 5.259E+1 1.554E+1
D_2 [mum^2/s] 4.645E-1 1.835E-1 2.651E-1 2.218E-1 3.303E-1 1.399E-1 4.287E-1 1.380E-1 1.118E+0 1.193E+0 2.503E-1 7.677E-2
alpha_2 1.291E+0 2.470E-1 1.323E+0 2.796E-1 1.333E+0 3.218E-1 9.780E-1 1.684E-1 9.736E-1 2.710E-1 9.777E-1 2.795E-1
tauD_m [ms] 5.417E+0 2.632E+0 4.249E+1 3.498E+1 4.214E+0 2.649E+0 2.378E+0 1.389E+0 4.690E+1 5.853E+1 4.263E+0 2.673E+0
D_m [mum^2/s] 3.897E+0 1.347E+0 4.051E+0 1.354E+0 8.844E+0 6.390E+0 8.575E+0 6.986E+0 4.146E+0 2.053E+0 8.402E+0 3.363E+0
R [1/s] 3.610E+1 2.766E+1 1.706E+1 1.828E+1
CPM [kHz] 2.208E+0 6.465E-1 2.999E-1 1.872E-1 6.702E-1 7.036E-1 3.391E-1 1.967E-1
CPM_corr [kHz] 2.231E+0 6.532E-1 4.610E-1 2.402E-1 8.689E-1 7.312E-1 3.849E-1 2.125E-1
Int [kHz] 4.201E+2 4.689E+2 1.766E+2 5.436E+1 2.811E+1 4.222E+1 8.809E+1 4.337E+1
Int_corr [kHz] 4.752E+2 5.048E+2 2.722E+2 7.180E+1 2.863E+1 4.351E+1 1.237E+2 6.177E+1
# samples 19 19 13 13 13 13 12 12 5 5 4 4
# cells 8

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 1306.243 - sd = 1323.131Int_corr [kHz] - Ch1 - Nuc : ave = 475.234 - sd = 504.816r = -0.154 - r^2 = 0.024
f_1 - Ch1 - Nuc : ave = 0.889 - sd = 0.084D_1 [mum^2/s] - Ch1 - Nuc : ave = 4.333 - sd = 1.483r = -0.571 - r^2 = 0.326
f_2 - Ch1 - Nuc : ave = 0.111 - sd = 0.084D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.465 - sd = 0.183r = 0.343 - r^2 = 0.117
CPM_corr [kHz] - Ch1 - Nuc : ave = 2.231 - sd = 0.653D_m [mum^2/s] - Ch1 - Nuc : ave = 3.897 - sd = 1.347r = 0.281 - r^2 = 0.079
c_corr [nM] - Ch1 - Nuc : ave = 1306.243 - sd = 1323.131ratioG_corr - Cross - Nuc : ave = 0.047 - sd = 0.105r = -0.130 - r^2 = 0.017
c_corr [nM] - Ch1 - Cyto : ave = 275.012 - sd = 341.697Int_corr [kHz] - Ch1 - Cyto : ave = 28.630 - sd = 43.514r = 0.472 - r^2 = 0.223
f_1 - Ch1 - Cyto : ave = 0.968 - sd = 0.045D_1 [mum^2/s] - Ch1 - Cyto : ave = 8.815 - sd = 7.067r = -0.278 - r^2 = 0.077
f_2 - Ch1 - Cyto : ave = 0.032 - sd = 0.045D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.429 - sd = 0.138r = 0.570 - r^2 = 0.325
CPM_corr [kHz] - Ch1 - Cyto : ave = 0.869 - sd = 0.731D_m [mum^2/s] - Ch1 - Cyto : ave = 8.575 - sd = 6.986r = -0.740 - r^2 = 0.547