Automated F(C)CS analysis of clone # 2318

Created on 12/08/2014
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Gene and protein information

Clone # 2318
Gene symbol: ZSCAN22
Description: zinc finger and SCAN domain containing 22
Molecular weight (with FP): 81.6 kDa
Localisation: granular nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
2318_img_ch1.png2318_img_ch2.png


Point 1, intensity traces and correlation functions
2318_itr_1.png2318_cor_1.png

Point 2, intensity traces and correlation functions
2318_itr_2.png2318_cor_2.png

Point 3, intensity traces and correlation functions
2318_itr_3.png2318_cor_3.png

Point 4, intensity traces and correlation functions
2318_itr_4.png2318_cor_4.png

Point 5, intensity traces and correlation functions
2318_itr_5.png2318_cor_5.png

Point 6, intensity traces and correlation functions
2318_itr_6.png2318_cor_6.png

Sessions

1 20111117\U02--V01--2318
2 20111125\U02--V01--2318
3 20111129\U02--V01--2318
4 20111206\U02--V01--2318
5 20120127\U01--V00--2318

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 9.590E+1 4.194E+1 9.266E+2 4.763E+2 1.333E+1 1.383E+1 3.773E+1 5.065E+1 3.172E+2 2.106E+2 1.866E+1 2.179E+1
N_corr 1.122E+2 7.393E+1 1.246E+3 8.311E+2 2.419E+1 7.401E+1 4.296E+1 5.616E+1 4.129E+2 3.216E+2 3.127E+1 5.037E+1
c [nM] 5.964E+2 2.608E+2 4.298E+3 2.209E+3 6.851E+1 7.112E+1 2.346E+2 3.150E+2 1.471E+3 9.768E+2 9.591E+1 1.120E+2
c_corr [nM] 6.980E+2 4.598E+2 5.778E+3 3.855E+3 1.244E+2 3.805E+2 2.672E+2 3.493E+2 1.915E+3 1.492E+3 1.608E+2 2.589E+2
ratioG 1.149E-1 7.723E-2 3.665E-1 2.595E-1
ratioG_corr 2.972E-2 9.140E-2 1.061E-1 5.341E-2
f_1 8.135E-1 1.625E-1 4.025E-1 2.145E-1 9.399E-1 1.128E-1 9.049E-1 7.955E-2 7.364E-1 2.796E-1 9.551E-1 6.686E-2
tauD_1 [ms] 2.461E+0 1.952E+0 1.860E+0 4.441E-16 2.025E+0 1.570E+0 4.084E-1 3.390E-1 1.860E+0 4.441E-16 1.149E+0 1.080E+0
D_1 [mum^2/s] 8.975E+0 9.414E+0 6.926E+0 8.882E-16 1.428E+1 2.230E+1 3.492E+1 1.592E+1 6.926E+0 0.000E+0 3.834E+1 3.721E+1
alpha_1 9.877E-1 9.297E-2 9.200E-1 0.000E+0 8.478E-1 2.025E-1 1.021E+0 1.029E-1 9.200E-1 0.000E+0 8.897E-1 2.021E-1
f_2 1.865E-1 1.625E-1 5.975E-1 2.145E-1 6.013E-2 1.128E-1 9.511E-2 7.955E-2 2.636E-1 2.796E-1 4.493E-2 6.686E-2
tauD_2 [ms] 3.259E+1 2.831E+1 9.055E+1 1.250E+2 8.785E+1 8.137E+1 2.410E+1 5.166E+1 1.170E+2 1.637E+2 4.847E+1 3.610E+1
D_2 [mum^2/s] 5.648E-1 4.913E-1 7.147E-1 1.025E+0 2.273E-1 1.996E-1 1.144E+0 1.444E+0 7.154E-1 1.093E+0 4.857E-1 3.856E-1
alpha_2 1.373E+0 3.048E-1 1.302E+0 3.394E-1 1.208E+0 2.820E-1 1.072E+0 1.580E-1 1.226E+0 4.162E-1 9.734E-1 2.018E-1
tauD_m [ms] 8.016E+0 7.824E+0 5.110E+1 6.993E+1 9.183E+0 2.267E+1 4.515E+0 1.787E+1 3.384E+1 4.997E+1 4.935E+0 6.802E+0
D_m [mum^2/s] 7.521E+0 8.738E+0 3.322E+0 1.197E+0 1.324E+1 2.032E+1 3.186E+1 1.453E+1 5.453E+0 1.318E+0 3.760E+1 3.716E+1
R [1/s] 2.318E+1 3.724E+1 4.396E+1 5.527E+1
CPM [kHz] 1.717E+0 5.593E-1 4.151E-1 3.639E-1 1.065E+0 5.580E-1 3.241E-1 1.850E-1
CPM_corr [kHz] 1.741E+0 5.576E-1 4.902E-1 4.235E-1 1.166E+0 5.440E-1 6.621E-1 5.638E-1
Int [kHz] 1.784E+2 1.046E+2 1.921E+2 5.860E+1 4.606E+1 7.179E+1 6.945E+1 4.587E+1
Int_corr [kHz] 2.025E+2 1.181E+2 2.912E+2 1.015E+2 5.461E+1 8.076E+1 1.090E+2 7.316E+1
# samples 155 155 133 133 117 117 106 106 14 14 10 10
# cells 71

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 697.999 - sd = 459.793Int_corr [kHz] - Ch1 - Nuc : ave = 202.469 - sd = 118.060r = 0.766 - r^2 = 0.587
f_1 - Ch1 - Nuc : ave = 0.814 - sd = 0.162D_1 [mum^2/s] - Ch1 - Nuc : ave = 8.975 - sd = 9.414r = -0.107 - r^2 = 0.012
f_2 - Ch1 - Nuc : ave = 0.186 - sd = 0.162D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.565 - sd = 0.491r = -0.073 - r^2 = 0.005
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.741 - sd = 0.558D_m [mum^2/s] - Ch1 - Nuc : ave = 7.521 - sd = 8.738r = -0.261 - r^2 = 0.068
c_corr [nM] - Ch1 - Nuc : ave = 697.999 - sd = 459.793ratioG_corr - Cross - Nuc : ave = 0.030 - sd = 0.091r = 0.013 - r^2 = 0.000
c_corr [nM] - Ch1 - Cyto : ave = 267.200 - sd = 349.261Int_corr [kHz] - Ch1 - Cyto : ave = 54.613 - sd = 80.757r = 0.616 - r^2 = 0.379
f_1 - Ch1 - Cyto : ave = 0.905 - sd = 0.080D_1 [mum^2/s] - Ch1 - Cyto : ave = 34.922 - sd = 15.919r = 0.331 - r^2 = 0.109
f_2 - Ch1 - Cyto : ave = 0.095 - sd = 0.080D_2 [mum^2/s] - Ch1 - Cyto : ave = 1.144 - sd = 1.444r = -0.304 - r^2 = 0.092
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.166 - sd = 0.544D_m [mum^2/s] - Ch1 - Cyto : ave = 31.863 - sd = 14.527r = 0.251 - r^2 = 0.063