Automated F(C)CS analysis of clone # 2093

Created on 12/08/2014
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Gene and protein information

Clone # 2093
Gene symbol: MECP2
Description: methyl CpG binding protein 2 (Rett syndrome)
Molecular weight (with FP): 79.4 kDa
Localisation: granular nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
2093_img_ch1.png2093_img_ch2.png


Point 1, intensity traces and correlation functions
2093_itr_1.png2093_cor_1.png

Point 2, intensity traces and correlation functions
2093_itr_2.png2093_cor_2.png

Point 3, intensity traces and correlation functions
2093_itr_3.png2093_cor_3.png

Point 4, intensity traces and correlation functions
2093_itr_4.png2093_cor_4.png

Point 5, intensity traces and correlation functions
2093_itr_5.png2093_cor_5.png

Point 6, intensity traces and correlation functions
2093_itr_6.png2093_cor_6.png

Sessions

1 20111117\U03--V00--2093
2 20111206\U01--V01--2093
3 20111207\U02--V01--2093
4 20111216\U02--V00--2093

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.328E+2 8.212E+1 1.293E+3 6.137E+2 1.308E+1 1.606E+1 4.232E+1 6.493E+1 4.156E+2 2.589E+2 3.759E+1 4.672E+1
N_corr 1.805E+2 1.172E+2 1.992E+3 1.077E+3 2.525E+1 3.347E+1 4.636E+1 7.656E+1 4.822E+2 3.659E+2 7.375E+1 7.998E+1
c [nM] 8.261E+2 5.107E+2 5.999E+3 2.847E+3 6.722E+1 8.258E+1 2.632E+2 4.038E+2 1.928E+3 1.201E+3 1.933E+2 2.402E+2
c_corr [nM] 1.122E+3 7.287E+2 9.239E+3 4.997E+3 1.298E+2 1.721E+2 2.884E+2 4.761E+2 2.237E+3 1.697E+3 3.792E+2 4.112E+2
ratioG 8.732E-2 6.218E-2 3.028E-1 1.819E-1
ratioG_corr 5.136E-2 5.927E-2 2.367E-1 2.249E-1
f_1 6.279E-1 1.383E-1 4.115E-1 2.302E-1 8.884E-1 1.572E-1 9.348E-1 8.541E-2 7.115E-1 3.454E-1 9.523E-1 9.359E-2
tauD_1 [ms] 2.827E+0 2.187E+0 1.860E+0 4.441E-16 5.864E+0 8.176E+0 5.928E-1 7.085E-1 1.860E+0 0.000E+0 2.496E+0 1.316E+0
D_1 [mum^2/s] 7.800E+0 1.325E+1 6.926E+0 1.776E-15 9.186E+0 2.029E+1 3.851E+1 2.881E+1 6.926E+0 8.882E-16 8.381E+0 8.305E+0
alpha_1 9.366E-1 1.086E-1 9.200E-1 4.441E-16 9.008E-1 2.514E-1 9.412E-1 1.526E-1 9.200E-1 0.000E+0 1.042E+0 2.129E-1
f_2 3.721E-1 1.383E-1 5.885E-1 2.302E-1 1.116E-1 1.572E-1 6.522E-2 8.541E-2 2.885E-1 3.454E-1 4.771E-2 9.359E-2
tauD_2 [ms] 4.951E+1 3.827E+1 7.847E+1 9.140E+1 1.492E+2 1.239E+2 1.872E+1 2.451E+1 8.406E+1 1.568E+2 8.700E+1 1.044E+2
D_2 [mum^2/s] 4.152E-1 1.172E+0 5.823E-1 7.484E-1 1.166E-1 7.123E-2 1.005E+0 5.932E-1 1.068E+0 1.440E+0 2.956E-1 3.491E-1
alpha_2 1.712E+0 3.060E-1 1.271E+0 2.997E-1 1.294E+0 3.473E-1 1.167E+0 2.000E-1 1.190E+0 3.015E-1 1.146E+0 2.358E-1
tauD_m [ms] 1.916E+1 1.166E+1 4.317E+1 5.427E+1 2.552E+1 4.148E+1 2.349E+0 5.017E+0 1.870E+1 4.638E+1 1.474E+1 3.188E+1
D_m [mum^2/s] 5.565E+0 1.257E+1 3.247E+0 1.397E+0 7.914E+0 1.716E+1 3.680E+1 2.849E+1 5.632E+0 1.445E+0 7.947E+0 8.080E+0
R [1/s] 1.053E+1 2.440E+1 2.410E+1 5.532E+1
CPM [kHz] 9.004E-1 3.461E-1 4.489E-1 4.931E-1 6.395E-1 9.543E-1 2.900E-1 1.777E-1
CPM_corr [kHz] 9.176E-1 3.486E-1 4.939E-1 5.448E-1 8.908E-1 1.015E+0 1.324E+0 3.853E+0
Int [kHz] 1.165E+2 6.942E+1 2.399E+2 6.945E+1 2.029E+1 3.464E+1 9.393E+1 5.935E+1
Int_corr [kHz] 1.571E+2 9.071E+1 3.964E+2 1.306E+2 2.843E+1 8.424E+1 1.311E+2 8.914E+1
# samples 246 246 192 192 161 161 169 169 19 19 15 15
# cells 103

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 1122.382 - sd = 728.655Int_corr [kHz] - Ch1 - Nuc : ave = 157.138 - sd = 90.715r = 0.563 - r^2 = 0.317
f_1 - Ch1 - Nuc : ave = 0.628 - sd = 0.138D_1 [mum^2/s] - Ch1 - Nuc : ave = 7.800 - sd = 13.248r = -0.323 - r^2 = 0.105
f_2 - Ch1 - Nuc : ave = 0.372 - sd = 0.138D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.415 - sd = 1.172r = 0.316 - r^2 = 0.100
CPM_corr [kHz] - Ch1 - Nuc : ave = 0.918 - sd = 0.349D_m [mum^2/s] - Ch1 - Nuc : ave = 5.565 - sd = 12.568r = -0.163 - r^2 = 0.026
c_corr [nM] - Ch1 - Nuc : ave = 1122.382 - sd = 728.655ratioG_corr - Cross - Nuc : ave = 0.051 - sd = 0.059r = 0.187 - r^2 = 0.035
c_corr [nM] - Ch1 - Cyto : ave = 288.351 - sd = 476.124Int_corr [kHz] - Ch1 - Cyto : ave = 28.433 - sd = 84.243r = 0.687 - r^2 = 0.472
f_1 - Ch1 - Cyto : ave = 0.935 - sd = 0.085D_1 [mum^2/s] - Ch1 - Cyto : ave = 38.512 - sd = 28.814r = 0.111 - r^2 = 0.012
f_2 - Ch1 - Cyto : ave = 0.065 - sd = 0.085D_2 [mum^2/s] - Ch1 - Cyto : ave = 1.005 - sd = 0.593r = -0.198 - r^2 = 0.039
CPM_corr [kHz] - Ch1 - Cyto : ave = 0.891 - sd = 1.015D_m [mum^2/s] - Ch1 - Cyto : ave = 36.804 - sd = 28.487r = 0.049 - r^2 = 0.002