Automated F(C)CS analysis of clone # 2018hi

Created on 12/08/2014
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Gene and protein information

Clone # 2018hi
Gene symbol: FAM50A
Description: family with sequence similarity 50, member A
Molecular weight (with FP): 67.2 kDa
Localisation: homogeneous nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
2018hi_img_ch1.png2018hi_img_ch2.png


Point 1, intensity traces and correlation functions
2018hi_itr_1.png2018hi_cor_1.png

Point 2, intensity traces and correlation functions
2018hi_itr_2.png2018hi_cor_2.png

Point 3, intensity traces and correlation functions
2018hi_itr_3.png2018hi_cor_3.png

Point 4, intensity traces and correlation functions
2018hi_itr_4.png2018hi_cor_4.png

Point 5, intensity traces and correlation functions
2018hi_itr_5.png2018hi_cor_5.png

Point 6, intensity traces and correlation functions
2018hi_itr_6.png2018hi_cor_6.png

Sessions

1 20130109\U04--V00--2018hi
2 20130111\U01--V00--2018hi

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 7.351E+2 4.367E+2 1.077E+3 5.321E+2 2.709E+2 1.915E+2 1.096E+2 2.926E+2 8.767E+2 3.168E+2 2.803E+2 0.000E+0
N_corr 7.489E+2 4.433E+2 1.020E+3 5.026E+2 2.837E+2 1.983E+2 1.874E+2 5.420E+2 9.638E+2 3.821E+2 2.591E+2 0.000E+0
c [nM] 4.572E+3 2.716E+3 4.996E+3 2.468E+3 1.393E+3 9.845E+2 6.816E+2 1.820E+3 4.066E+3 1.469E+3 1.441E+3 0.000E+0
c_corr [nM] 4.658E+3 2.757E+3 4.731E+3 2.331E+3 1.459E+3 1.019E+3 1.165E+3 3.371E+3 4.470E+3 1.772E+3 1.332E+3 0.000E+0
ratioG 2.755E-1 1.050E-1 3.716E-1 0.000E+0
ratioG_corr 5.029E-2 8.387E-2 8.279E-2 0.000E+0
f_1 9.551E-1 4.774E-2 4.969E-1 2.974E-1 9.924E-1 2.627E-2 9.463E-1 7.014E-2 6.386E-1 1.638E-1 1.000E+0 0.000E+0
tauD_1 [ms] 1.210E+0 9.831E-1 1.860E+0 0.000E+0 5.180E-1 4.631E-1 5.134E-1 4.799E-1 1.860E+0 0.000E+0 3.768E-1 0.000E+0
D_1 [mum^2/s] 1.244E+1 5.532E+0 6.926E+0 0.000E+0 4.137E+1 3.027E+1 3.526E+1 2.795E+1 6.926E+0 0.000E+0 3.182E+1 0.000E+0
alpha_1 1.046E+0 9.629E-2 9.200E-1 1.110E-16 9.974E-1 1.930E-1 1.087E+0 1.395E-1 9.200E-1 0.000E+0 7.503E-1 0.000E+0
f_2 4.490E-2 4.774E-2 5.031E-1 2.974E-1 7.582E-3 2.627E-2 5.369E-2 7.014E-2 3.614E-1 1.638E-1 0.000E+0 0.000E+0
tauD_2 [ms] 1.691E+1 9.200E+0 4.348E+1 3.797E+1 4.068E+1 1.616E+1 1.747E+1 1.705E+1 1.782E+1 2.094E+0 1.987E+1 0.000E+0
D_2 [mum^2/s] 7.623E-1 2.934E-1 7.151E-1 7.345E-1 3.382E-1 1.182E-1 9.647E-1 6.417E-1 7.329E-1 8.648E-2 6.033E-1 0.000E+0
alpha_2 1.128E+0 1.271E-1 1.169E+0 3.327E-1 1.087E+0 5.046E-2 1.100E+0 5.091E-2 1.119E+0 1.955E-1 1.077E+0 0.000E+0
tauD_m [ms] 1.927E+0 1.221E+0 1.735E+1 1.529E+1 7.629E-1 8.921E-1 2.181E+0 5.564E+0 7.509E+0 2.361E+0 3.768E-1 0.000E+0
D_m [mum^2/s] 1.186E+1 5.263E+0 3.918E+0 1.695E+0 4.104E+1 3.006E+1 3.335E+1 2.693E+1 4.692E+0 1.002E+0 3.182E+1 0.000E+0
R [1/s] 4.588E+1 4.629E+1 3.900E+1 0.000E+0
CPM [kHz] 5.873E-1 9.340E-2 2.398E-1 1.245E-1 2.796E-1 1.337E-1 2.253E-1 8.418E-2
CPM_corr [kHz] 5.915E-1 9.309E-2 3.418E-1 1.597E-1 4.738E-1 2.559E-1 2.935E-1 6.519E-2
Int [kHz] 4.574E+2 2.673E+2 1.923E+2 4.913E+1 4.468E+1 1.219E+2 1.519E+2 1.818E+1
Int_corr [kHz] 4.682E+2 2.719E+2 2.717E+2 7.492E+1 7.173E+1 1.869E+2 2.309E+2 4.667E+1
# samples 54 54 41 41 32 32 34 34 4 4 1 1
# cells 33

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 4657.654 - sd = 2756.920Int_corr [kHz] - Ch1 - Nuc : ave = 468.164 - sd = 271.920r = 0.964 - r^2 = 0.929
f_1 - Ch1 - Nuc : ave = 0.955 - sd = 0.048D_1 [mum^2/s] - Ch1 - Nuc : ave = 12.443 - sd = 5.532r = -0.023 - r^2 = 0.001
f_2 - Ch1 - Nuc : ave = 0.045 - sd = 0.048D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.762 - sd = 0.293r = -0.407 - r^2 = 0.165
CPM_corr [kHz] - Ch1 - Nuc : ave = 0.592 - sd = 0.093D_m [mum^2/s] - Ch1 - Nuc : ave = 11.862 - sd = 5.263r = 0.319 - r^2 = 0.102
c_corr [nM] - Ch1 - Nuc : ave = 4657.654 - sd = 2756.920ratioG_corr - Cross - Nuc : ave = 0.050 - sd = 0.084r = 0.179 - r^2 = 0.032
c_corr [nM] - Ch1 - Cyto : ave = 1165.326 - sd = 3370.996Int_corr [kHz] - Ch1 - Cyto : ave = 71.726 - sd = 186.935r = 0.791 - r^2 = 0.626
f_1 - Ch1 - Cyto : ave = 0.946 - sd = 0.070D_1 [mum^2/s] - Ch1 - Cyto : ave = 35.258 - sd = 27.953r = -0.521 - r^2 = 0.271
f_2 - Ch1 - Cyto : ave = 0.054 - sd = 0.070D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.965 - sd = 0.642r = 0.318 - r^2 = 0.101
CPM_corr [kHz] - Ch1 - Cyto : ave = 0.474 - sd = 0.256D_m [mum^2/s] - Ch1 - Cyto : ave = 33.352 - sd = 26.930r = 0.514 - r^2 = 0.264