Automated F(C)CS analysis of clone # 2018

Created on 12/08/2014
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Gene and protein information

Clone # 2018
Gene symbol: FAM50A
Description: family with sequence similarity 50, member A
Molecular weight (with FP): 67.2 kDa
Localisation: homogeneous nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
2018_img_ch1.png2018_img_ch2.png


Point 1, intensity traces and correlation functions
2018_itr_1.png2018_cor_1.png

Point 2, intensity traces and correlation functions
2018_itr_2.png2018_cor_2.png

Point 3, intensity traces and correlation functions
2018_itr_3.png2018_cor_3.png

Point 4, intensity traces and correlation functions
2018_itr_4.png2018_cor_4.png

Point 5, intensity traces and correlation functions
2018_itr_5.png2018_cor_5.png

Point 6, intensity traces and correlation functions
2018_itr_6.png2018_cor_6.png

Sessions

1 20110914\U02--V00--2018
2 20110920\U02--V00--2018
3 20110922\U01--V01--2018
4 20110927\U01--V01--2018
5 20110930\U01--V01--2018
6 20111117\U02--V00--2018

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.939E+2 2.500E+2 1.144E+3 6.454E+2 5.415E+1 1.404E+2 2.682E+1 4.327E+1 4.299E+2 3.367E+2 2.190E+1 2.726E+1
N_corr 2.155E+2 2.753E+2 1.260E+3 6.575E+2 6.540E+1 1.733E+2 4.079E+1 8.635E+1 6.397E+2 4.724E+2 1.168E+2 2.085E+2
c [nM] 1.206E+3 1.555E+3 5.307E+3 2.994E+3 2.784E+2 7.220E+2 1.668E+2 2.691E+2 1.994E+3 1.562E+3 1.126E+2 1.402E+2
c_corr [nM] 1.340E+3 1.712E+3 5.846E+3 3.050E+3 3.362E+2 8.908E+2 2.537E+2 5.371E+2 2.967E+3 2.191E+3 6.005E+2 1.072E+3
ratioG 1.534E-1 1.129E-1 2.278E-1 1.567E-1
ratioG_corr 2.158E-2 9.291E-2 2.543E-1 3.791E-1
f_1 9.220E-1 6.673E-2 5.243E-1 2.020E-1 9.890E-1 4.651E-2 9.299E-1 6.954E-2 7.322E-1 2.873E-1 9.217E-1 1.873E-1
tauD_1 [ms] 1.338E+0 9.045E-1 1.814E+0 2.556E-2 8.677E-1 7.383E-1 5.602E-1 4.326E-1 5.072E+0 1.201E+1 1.096E+0 7.793E-1
D_1 [mum^2/s] 1.141E+1 9.010E+0 7.102E+0 9.833E-2 2.607E+1 2.275E+1 2.692E+1 1.665E+1 6.543E+0 1.783E+0 1.682E+1 1.040E+1
alpha_1 1.014E+0 9.358E-2 9.200E-1 0.000E+0 9.312E-1 1.798E-1 1.011E+0 1.283E-1 9.293E-1 1.487E-1 9.723E-1 2.323E-1
f_2 7.795E-2 6.673E-2 4.757E-1 2.020E-1 1.103E-2 4.651E-2 7.013E-2 6.954E-2 2.678E-1 2.873E-1 7.830E-2 1.873E-1
tauD_2 [ms] 2.488E+1 3.901E+1 1.071E+2 1.307E+2 5.046E+1 4.825E+1 1.865E+1 4.515E+1 1.072E+2 1.507E+2 1.250E+2 2.163E+2
D_2 [mum^2/s] 7.326E-1 3.668E-1 5.086E-1 7.201E-1 3.711E-1 2.117E-1 1.015E+0 5.035E-1 8.075E-1 9.317E-1 3.760E-1 3.464E-1
alpha_2 1.173E+0 2.745E-1 1.288E+0 3.135E-1 1.084E+0 1.442E-1 1.037E+0 1.078E-1 1.251E+0 2.770E-1 1.143E+0 1.804E-1
tauD_m [ms] 4.246E+0 1.064E+1 5.336E+1 7.078E+1 2.930E+0 1.317E+1 3.951E+0 2.466E+1 6.872E+1 1.106E+2 5.088E+1 1.315E+2
D_m [mum^2/s] 1.067E+1 8.876E+0 4.003E+0 1.286E+0 2.594E+1 2.275E+1 2.532E+1 1.645E+1 4.671E+0 2.261E+0 1.625E+1 1.065E+1
R [1/s] 6.924E+1 4.974E+1 4.783E+1 4.829E+1
CPM [kHz] 1.398E+0 4.462E-1 3.403E-1 2.709E-1 6.755E-1 7.705E-1 5.552E-1 7.132E-1
CPM_corr [kHz] 1.418E+0 4.451E-1 4.326E-1 3.222E-1 9.621E-1 8.302E-1 7.391E-1 9.745E-1
Int [kHz] 2.866E+2 2.907E+2 1.930E+2 7.225E+1 1.897E+1 5.235E+1 9.933E+1 5.961E+1
Int_corr [kHz] 3.142E+2 3.117E+2 2.802E+2 1.189E+2 3.685E+1 1.070E+2 1.893E+2 1.355E+2
# samples 98 98 63 63 57 57 127 127 15 15 8 8
# cells 60

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 1340.382 - sd = 1712.349Int_corr [kHz] - Ch1 - Nuc : ave = 314.201 - sd = 311.747r = 0.836 - r^2 = 0.699
f_1 - Ch1 - Nuc : ave = 0.922 - sd = 0.067D_1 [mum^2/s] - Ch1 - Nuc : ave = 11.413 - sd = 9.010r = 0.333 - r^2 = 0.111
f_2 - Ch1 - Nuc : ave = 0.078 - sd = 0.067D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.733 - sd = 0.367r = -0.420 - r^2 = 0.176
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.418 - sd = 0.445D_m [mum^2/s] - Ch1 - Nuc : ave = 10.670 - sd = 8.876r = 0.360 - r^2 = 0.129
c_corr [nM] - Ch1 - Nuc : ave = 1340.382 - sd = 1712.349ratioG_corr - Cross - Nuc : ave = 0.022 - sd = 0.093r = 0.184 - r^2 = 0.034
c_corr [nM] - Ch1 - Cyto : ave = 253.685 - sd = 537.061Int_corr [kHz] - Ch1 - Cyto : ave = 36.853 - sd = 107.033r = 0.443 - r^2 = 0.196
f_1 - Ch1 - Cyto : ave = 0.930 - sd = 0.070D_1 [mum^2/s] - Ch1 - Cyto : ave = 26.921 - sd = 16.655r = 0.020 - r^2 = 0.000
f_2 - Ch1 - Cyto : ave = 0.070 - sd = 0.070D_2 [mum^2/s] - Ch1 - Cyto : ave = 1.015 - sd = 0.504r = -0.068 - r^2 = 0.005
CPM_corr [kHz] - Ch1 - Cyto : ave = 0.962 - sd = 0.830D_m [mum^2/s] - Ch1 - Cyto : ave = 25.323 - sd = 16.448r = 0.143 - r^2 = 0.020