Automated F(C)CS analysis of clone # 1882

Created on 12/08/2014
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Gene and protein information

Clone # 1882
Gene symbol: H2AFB3
Description: H2A histone family, member B3
Molecular weight (with FP): 39.7 kDa
Localisation: granular nucleus, ave. nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
1882_img_ch1.png1882_img_ch2.png


Point 1, intensity traces and correlation functions
1882_itr_1.png1882_cor_1.png

Point 2, intensity traces and correlation functions
1882_itr_2.png1882_cor_2.png

Point 3, intensity traces and correlation functions
1882_itr_3.png1882_cor_3.png

Point 4, intensity traces and correlation functions
1882_itr_4.png1882_cor_4.png

Point 5, intensity traces and correlation functions
1882_itr_5.png1882_cor_5.png

Point 6, intensity traces and correlation functions
1882_itr_6.png1882_cor_6.png

Sessions

1 20110831\U01--V00--1882
2 20110831\U02--V00--1882
3 20110831\U03--V00--1882
4 20110906\U02--V00--1882

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.531E+2 1.853E+2 9.609E+2 4.656E+2 2.474E+1 4.508E+1 2.541E+1 3.955E+1 2.603E+2 2.818E+2 1.943E+1 3.119E+1
N_corr 1.973E+2 2.479E+2 1.192E+3 6.014E+2 4.335E+1 1.022E+2 3.429E+1 7.462E+1 3.055E+2 3.858E+2 7.278E+1 3.038E+2
c [nM] 9.524E+2 1.153E+3 4.457E+3 2.159E+3 1.272E+2 2.318E+2 1.581E+2 2.460E+2 1.207E+3 1.307E+3 9.991E+1 1.603E+2
c_corr [nM] 1.227E+3 1.542E+3 5.527E+3 2.790E+3 2.229E+2 5.256E+2 2.133E+2 4.641E+2 1.417E+3 1.790E+3 3.742E+2 1.562E+3
ratioG 1.260E-1 9.542E-2 3.716E-1 2.125E-1
ratioG_corr 5.618E-2 7.966E-2 1.959E-1 2.083E-1
f_1 6.683E-1 1.629E-1 4.645E-1 1.915E-1 9.197E-1 1.104E-1 8.179E-1 7.230E-2 1.000E+0 0.000E+0 9.609E-1 7.987E-2
tauD_1 [ms] 2.478E+0 1.740E+0 1.793E+0 1.188E-1 3.325E+0 5.300E+0 8.283E-1 5.291E-1 7.107E+0 1.204E+1 1.125E+0 1.131E+0
D_1 [mum^2/s] 5.975E+0 3.942E+0 7.215E+0 4.573E-1 1.464E+1 2.532E+1 1.676E+1 8.881E+0 1.028E+1 2.118E+1 2.402E+1 1.903E+1
alpha_1 9.745E-1 1.188E-1 9.242E-1 8.122E-3 7.191E-1 1.969E-1 9.688E-1 1.692E-1 8.692E-1 2.520E-1 7.597E-1 2.660E-1
f_2 3.317E-1 1.629E-1 5.355E-1 1.915E-1 8.029E-2 1.104E-1 1.821E-1 7.230E-2 0.000E+0 0.000E+0 3.906E-2 7.987E-2
tauD_2 [ms] 3.398E+1 1.869E+1 1.161E+2 1.237E+2 1.068E+2 7.825E+1 2.564E+1 1.332E+1 2.227E+1 2.785E+1 5.311E+1 5.452E+1
D_2 [mum^2/s] 3.770E-1 1.484E-1 3.805E-1 6.885E-1 1.646E-1 9.535E-2 4.987E-1 2.036E-1 1.914E+0 1.068E+0 3.497E-1 1.756E-1
alpha_2 1.615E+0 2.805E-1 1.351E+0 3.163E-1 1.314E+0 2.686E-1 1.245E+0 2.233E-1 1.278E+0 4.479E-1 1.259E+0 2.588E-1
tauD_m [ms] 1.232E+1 7.319E+0 6.430E+1 7.800E+1 1.053E+1 1.616E+1 5.361E+0 3.284E+0 7.107E+0 1.204E+1 4.069E+0 1.243E+1
D_m [mum^2/s] 3.814E+0 2.198E+0 3.577E+0 1.288E+0 1.264E+1 2.085E+1 1.380E+1 7.487E+0 1.028E+1 2.118E+1 2.330E+1 1.903E+1
R [1/s] 2.521E+1 2.091E+1 1.285E+1 2.414E+1
CPM [kHz] 1.138E+0 3.305E-1 3.512E-1 3.203E-1 8.667E-1 4.284E-1 2.289E-1 1.594E-1
CPM_corr [kHz] 1.167E+0 3.386E-1 4.039E-1 3.505E-1 1.104E+0 5.007E-1 4.633E-1 3.249E-1
Int [kHz] 1.661E+2 1.626E+2 1.557E+2 4.563E+1 2.189E+1 4.281E+1 5.008E+1 5.031E+1
Int_corr [kHz] 2.103E+2 2.082E+2 2.243E+2 7.329E+1 3.130E+1 6.458E+1 6.997E+1 7.465E+1
# samples 226 226 168 168 155 155 365 365 72 72 56 56
# cells 137

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 1226.961 - sd = 1541.860Int_corr [kHz] - Ch1 - Nuc : ave = 210.280 - sd = 208.194r = 0.870 - r^2 = 0.756
f_1 - Ch1 - Nuc : ave = 0.668 - sd = 0.163D_1 [mum^2/s] - Ch1 - Nuc : ave = 5.975 - sd = 3.942r = -0.417 - r^2 = 0.174
f_2 - Ch1 - Nuc : ave = 0.332 - sd = 0.163D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.377 - sd = 0.148r = 0.210 - r^2 = 0.044
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.167 - sd = 0.339D_m [mum^2/s] - Ch1 - Nuc : ave = 3.814 - sd = 2.198r = 0.006 - r^2 = 3.496E-5
c_corr [nM] - Ch1 - Nuc : ave = 1226.961 - sd = 1541.860ratioG_corr - Cross - Nuc : ave = 0.056 - sd = 0.080r = 0.520 - r^2 = 0.270
c_corr [nM] - Ch1 - Cyto : ave = 213.254 - sd = 464.061Int_corr [kHz] - Ch1 - Cyto : ave = 31.296 - sd = 64.579r = 0.781 - r^2 = 0.610
f_1 - Ch1 - Cyto : ave = 0.818 - sd = 0.072D_1 [mum^2/s] - Ch1 - Cyto : ave = 16.760 - sd = 8.881r = -0.064 - r^2 = 0.004
f_2 - Ch1 - Cyto : ave = 0.182 - sd = 0.072D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.499 - sd = 0.204r = 0.008 - r^2 = 6.455E-5
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.104 - sd = 0.501D_m [mum^2/s] - Ch1 - Cyto : ave = 13.801 - sd = 7.487r = 0.002 - r^2 = 4.412E-6