Automated F(C)CS analysis of clone # 1750

Created on 12/08/2014
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Gene and protein information

Clone # 1750
Gene symbol: APOLD1
Description: apolipoprotein L domain containing 1
Molecular weight (with FP): 57.5 kDa
Localisation: homogeneous nucleus, bright nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
1750_img_ch1.png1750_img_ch2.png


Point 1, intensity traces and correlation functions
1750_itr_1.png1750_cor_1.png

Point 2, intensity traces and correlation functions
1750_itr_2.png1750_cor_2.png

Point 3, intensity traces and correlation functions
1750_itr_3.png1750_cor_3.png

Point 4, intensity traces and correlation functions
1750_itr_4.png1750_cor_4.png

Point 5, intensity traces and correlation functions
1750_itr_5.png1750_cor_5.png

Point 6, intensity traces and correlation functions
1750_itr_6.png1750_cor_6.png

Sessions

1 20111111\U00--V01--1750
2 20111115\U00--V01--1750
3 20111129\U03--V00--1750
4 20111130\U02--V00--1750
5 20111201\U02--V00--1750
6 20111213\U03--V00--1750
7 20120228\U02--V00--1750

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 2.580E+2 2.448E+2 1.169E+3 5.916E+2 4.868E+1 6.757E+1 2.012E+1 3.620E+1 2.513E+2 2.174E+2 2.657E+1 4.571E+1
N_corr 2.952E+2 2.846E+2 1.454E+3 8.816E+2 6.978E+1 1.205E+2 1.873E+1 3.676E+1 2.922E+2 2.834E+2 3.308E+1 5.734E+1
c [nM] 1.605E+3 1.522E+3 5.420E+3 2.744E+3 2.503E+2 3.474E+2 1.252E+2 2.252E+2 1.166E+3 1.009E+3 1.366E+2 2.350E+2
c_corr [nM] 1.836E+3 1.770E+3 6.746E+3 4.089E+3 3.587E+2 6.193E+2 1.165E+2 2.286E+2 1.355E+3 1.314E+3 1.701E+2 2.948E+2
ratioG 1.830E-1 1.419E-1 2.432E-1 1.648E-1
ratioG_corr 3.765E-2 1.551E-1 1.924E-1 1.724E-1
f_1 9.070E-1 1.077E-1 4.104E-1 2.437E-1 9.686E-1 6.713E-2 8.652E-1 8.040E-2 6.330E-1 3.296E-1 9.717E-1 5.749E-2
tauD_1 [ms] 2.643E+0 2.059E+0 1.860E+0 4.441E-16 1.462E+0 8.741E-1 7.550E-1 4.631E-1 1.860E+0 0.000E+0 1.203E+0 1.103E+0
D_1 [mum^2/s] 5.169E+0 1.900E+0 6.926E+0 8.882E-16 1.490E+1 1.830E+1 1.868E+1 1.190E+1 6.926E+0 0.000E+0 3.338E+1 3.403E+1
alpha_1 1.025E+0 8.418E-2 9.200E-1 2.416E-4 9.292E-1 2.062E-1 9.459E-1 1.282E-1 9.200E-1 1.110E-16 8.854E-1 2.806E-1
f_2 9.305E-2 1.077E-1 5.896E-1 2.437E-1 3.136E-2 6.713E-2 1.348E-1 8.040E-2 3.670E-1 3.296E-1 2.827E-2 5.749E-2
tauD_2 [ms] 3.275E+1 3.140E+1 8.424E+1 1.120E+2 5.878E+1 4.894E+1 1.912E+1 7.934E+0 5.648E+1 7.138E+1 4.719E+1 1.875E+1
D_2 [mum^2/s] 4.904E-1 2.482E-1 7.088E-1 9.109E-1 2.917E-1 1.706E-1 6.478E-1 2.566E-1 1.209E+0 1.416E+0 3.104E-1 1.616E-1
alpha_2 1.319E+0 3.174E-1 1.240E+0 3.019E-1 1.121E+0 1.864E-1 1.218E+0 2.034E-1 1.282E+0 3.962E-1 1.123E+0 2.105E-1
tauD_m [ms] 6.131E+0 7.358E+0 4.049E+1 4.732E+1 4.758E+0 1.317E+1 3.205E+0 1.843E+0 1.251E+1 2.115E+1 2.313E+0 2.312E+0
D_m [mum^2/s] 4.745E+0 1.715E+0 3.383E+0 1.314E+0 1.452E+1 1.798E+1 1.601E+1 1.050E+1 5.144E+0 1.459E+0 3.165E+1 3.139E+1
R [1/s] 2.599E+1 2.764E+1 2.451E+1 2.534E+1
CPM [kHz] 1.736E+0 4.409E-1 4.125E-1 3.782E-1 6.452E-1 3.858E-1 2.732E-1 1.721E-1
CPM_corr [kHz] 1.748E+0 4.435E-1 5.484E-1 4.863E-1 9.679E-1 5.354E-1 3.387E-1 1.868E-1
Int [kHz] 4.249E+2 3.193E+2 2.067E+2 7.006E+1 1.086E+1 3.079E+1 5.757E+1 6.178E+1
Int_corr [kHz] 4.830E+2 3.680E+2 3.309E+2 1.197E+2 1.199E+1 2.918E+1 7.876E+1 9.588E+1
# samples 154 154 135 135 121 121 126 126 20 20 12 12
# cells 59

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 1835.919 - sd = 1769.900Int_corr [kHz] - Ch1 - Nuc : ave = 483.029 - sd = 368.020r = 0.901 - r^2 = 0.812
f_1 - Ch1 - Nuc : ave = 0.907 - sd = 0.108D_1 [mum^2/s] - Ch1 - Nuc : ave = 5.169 - sd = 1.900r = 0.078 - r^2 = 0.006
f_2 - Ch1 - Nuc : ave = 0.093 - sd = 0.108D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.490 - sd = 0.248r = -0.100 - r^2 = 0.010
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.748 - sd = 0.443D_m [mum^2/s] - Ch1 - Nuc : ave = 4.745 - sd = 1.715r = -0.154 - r^2 = 0.024
c_corr [nM] - Ch1 - Nuc : ave = 1835.919 - sd = 1769.900ratioG_corr - Cross - Nuc : ave = 0.038 - sd = 0.155r = 0.013 - r^2 = 0.000
c_corr [nM] - Ch1 - Cyto : ave = 116.501 - sd = 228.630Int_corr [kHz] - Ch1 - Cyto : ave = 11.991 - sd = 29.175r = 0.381 - r^2 = 0.145
f_1 - Ch1 - Cyto : ave = 0.865 - sd = 0.080D_1 [mum^2/s] - Ch1 - Cyto : ave = 18.683 - sd = 11.904r = -0.586 - r^2 = 0.343
f_2 - Ch1 - Cyto : ave = 0.135 - sd = 0.080D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.648 - sd = 0.257r = 0.114 - r^2 = 0.013
CPM_corr [kHz] - Ch1 - Cyto : ave = 0.968 - sd = 0.535D_m [mum^2/s] - Ch1 - Cyto : ave = 16.010 - sd = 10.497r = 0.196 - r^2 = 0.038