Automated F(C)CS analysis of clone # 1431

Created on 12/08/2014
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Gene and protein information

Clone # 1431
Gene symbol: MED23
Description: mediator complex subunit 23
Molecular weight (with FP): 183.5 kDa
Localisation: homogeneous nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
1431_img_ch1.png1431_img_ch2.png


Point 1, intensity traces and correlation functions
1431_itr_1.png1431_cor_1.png

Point 2, intensity traces and correlation functions
1431_itr_2.png1431_cor_2.png

Point 3, intensity traces and correlation functions
1431_itr_3.png1431_cor_3.png

Point 4, intensity traces and correlation functions
1431_itr_4.png1431_cor_4.png

Point 5, intensity traces and correlation functions
1431_itr_5.png1431_cor_5.png

Point 6, intensity traces and correlation functions
1431_itr_6.png1431_cor_6.png

Sessions

1 20111111\U01--V00--1431
2 20111115\U01--V00--1431
3 20111116\U01--V00--1431
4 20111209\U03--V00--1431
5 20120117\U02--V00--1431
6 20120214\U02--V00--1431
7 20120229\U00--V00--1431

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.329E+2 7.969E+1 1.288E+3 5.738E+2 1.952E+1 2.141E+1 2.578E+1 2.468E+1 2.323E+2 2.681E+2 1.048E+1 8.051E+0
N_corr 1.384E+2 8.354E+1 1.795E+3 9.427E+2 2.186E+1 2.486E+1 2.502E+1 2.462E+1 3.070E+2 4.655E+2 1.159E+1 9.064E+0
c [nM] 8.263E+2 4.956E+2 5.976E+3 2.662E+3 1.004E+2 1.101E+2 1.603E+2 1.535E+2 1.078E+3 1.243E+3 5.387E+1 4.139E+1
c_corr [nM] 8.606E+2 5.196E+2 8.327E+3 4.373E+3 1.124E+2 1.278E+2 1.556E+2 1.531E+2 1.424E+3 2.159E+3 5.959E+1 4.660E+1
ratioG 1.130E-1 6.470E-2 3.585E-1 2.196E-1
ratioG_corr 8.724E-3 3.896E-2 8.897E-2 6.224E-2
f_1 8.863E-1 1.557E-1 4.293E-1 2.253E-1 9.848E-1 4.312E-2 8.337E-1 2.008E-1 8.348E-1 2.663E-1 9.601E-1 7.340E-2
tauD_1 [ms] 1.125E+0 5.868E-1 1.860E+0 4.441E-16 8.127E-1 6.195E-1 9.325E-1 1.118E+0 1.860E+0 2.220E-16 7.673E-1 6.617E-1
D_1 [mum^2/s] 1.152E+1 5.950E+0 6.926E+0 4.441E-15 2.648E+1 2.429E+1 1.693E+1 9.244E+0 6.926E+0 8.882E-16 3.171E+1 2.373E+1
alpha_1 9.166E-1 1.011E-1 9.200E-1 2.096E-4 9.013E-1 2.069E-1 8.581E-1 1.285E-1 9.200E-1 1.110E-16 9.067E-1 2.261E-1
f_2 1.137E-1 1.557E-1 5.707E-1 2.253E-1 1.521E-2 4.312E-2 1.663E-1 2.008E-1 1.652E-1 2.663E-1 3.994E-2 7.340E-2
tauD_2 [ms] 1.761E+1 1.074E+1 7.573E+1 8.865E+1 5.583E+1 3.981E+1 1.307E+1 1.557E+1 7.739E+1 1.645E+2 4.038E+1 5.964E+1
D_2 [mum^2/s] 8.808E-1 1.323E+0 5.216E-1 6.615E-1 3.353E-1 4.678E-1 5.467E+0 8.116E+0 7.006E-1 9.850E-1 6.077E-1 3.971E-1
alpha_2 1.172E+0 2.080E-1 1.258E+0 2.756E-1 1.093E+0 1.163E-1 1.143E+0 1.676E-1 1.180E+0 3.442E-1 1.134E+0 2.152E-1
tauD_m [ms] 2.681E+0 2.576E+0 4.235E+1 5.484E+1 1.955E+0 7.110E+0 1.750E+0 2.917E+0 1.617E+1 3.969E+1 4.601E+0 1.708E+1
D_m [mum^2/s] 9.942E+0 3.626E+0 3.301E+0 1.401E+0 2.603E+1 2.371E+1 1.717E+1 8.495E+0 6.047E+0 1.319E+0 3.029E+1 2.260E+1
R [1/s] 6.752E+1 1.829E+2 6.699E+2 1.074E+3
CPM [kHz] 1.459E+0 3.604E-1 3.202E-1 2.568E-1 1.068E+0 5.001E-1 2.245E-1 2.314E-1
CPM_corr [kHz] 1.478E+0 3.610E-1 3.841E-1 3.072E-1 -6.156E-1 3.858E+1 5.082E-1 3.355E-1
Int [kHz] 2.091E+2 1.216E+2 1.991E+2 5.368E+1 2.772E+1 2.308E+1 4.350E+1 4.649E+1
Int_corr [kHz] 2.199E+2 1.281E+2 3.280E+2 1.088E+2 2.956E+1 2.379E+1 6.932E+1 8.553E+1
# samples 523 523 360 360 339 339 412 412 72 72 60 60
# cells 182

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 860.623 - sd = 519.573Int_corr [kHz] - Ch1 - Nuc : ave = 219.888 - sd = 128.080r = 0.831 - r^2 = 0.691
f_1 - Ch1 - Nuc : ave = 0.886 - sd = 0.156D_1 [mum^2/s] - Ch1 - Nuc : ave = 11.520 - sd = 5.950r = -0.239 - r^2 = 0.057
f_2 - Ch1 - Nuc : ave = 0.114 - sd = 0.156D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.881 - sd = 1.323r = 0.010 - r^2 = 0.000
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.478 - sd = 0.361D_m [mum^2/s] - Ch1 - Nuc : ave = 9.942 - sd = 3.626r = 0.201 - r^2 = 0.040
c_corr [nM] - Ch1 - Nuc : ave = 860.623 - sd = 519.573ratioG_corr - Cross - Nuc : ave = 0.009 - sd = 0.039r = 0.103 - r^2 = 0.011
c_corr [nM] - Ch1 - Cyto : ave = 155.626 - sd = 153.087Int_corr [kHz] - Ch1 - Cyto : ave = 29.557 - sd = 23.790r = 0.636 - r^2 = 0.404
f_1 - Ch1 - Cyto : ave = 0.834 - sd = 0.201D_1 [mum^2/s] - Ch1 - Cyto : ave = 16.929 - sd = 9.244r = 0.549 - r^2 = 0.301
f_2 - Ch1 - Cyto : ave = 0.166 - sd = 0.201D_2 [mum^2/s] - Ch1 - Cyto : ave = 5.467 - sd = 8.116r = 0.516 - r^2 = 0.266
CPM_corr [kHz] - Ch1 - Cyto : ave = -0.616 - sd = 38.582D_m [mum^2/s] - Ch1 - Cyto : ave = 17.170 - sd = 8.495r = 0.042 - r^2 = 0.002