Automated F(C)CS analysis of clone # 1383

Created on 12/08/2014
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Gene and protein information

Clone # 1383
Gene symbol: ZBTB20
Description: zinc finger and BTB domain containing 20
Molecular weight (with FP): 108.1 kDa
Localisation: granular nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
1383_img_ch1.png1383_img_ch2.png


Point 1, intensity traces and correlation functions
1383_itr_1.png1383_cor_1.png

Point 2, intensity traces and correlation functions
1383_itr_2.png1383_cor_2.png

Point 3, intensity traces and correlation functions
1383_itr_3.png1383_cor_3.png

Point 4, intensity traces and correlation functions
1383_itr_4.png1383_cor_4.png

Point 5, intensity traces and correlation functions
1383_itr_5.png1383_cor_5.png

Point 6, intensity traces and correlation functions
1383_itr_6.png1383_cor_6.png

Sessions

1 20120201\U01--V00--1383
2 20120217\U02--V00--1383
3 20120228\U01--V00--1383
4 20120309\U00--V00--1383

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 7.023E+1 9.856E+1 8.764E+2 4.040E+2 7.547E+0 3.290E+1 3.420E+1 7.464E+1 1.907E+2 1.990E+2 1.610E+1 5.439E+1
N_corr 8.417E+1 1.298E+2 1.206E+3 6.800E+2 1.402E+1 8.786E+1 4.007E+1 1.055E+2 2.166E+2 3.280E+2 6.865E+1 3.703E+2
c [nM] 4.368E+2 6.130E+2 4.065E+3 1.874E+3 3.880E+1 1.691E+2 2.127E+2 4.642E+2 8.846E+2 9.233E+2 8.276E+1 2.796E+2
c_corr [nM] 5.235E+2 8.074E+2 5.596E+3 3.154E+3 7.209E+1 4.517E+2 2.492E+2 6.561E+2 1.005E+3 1.521E+3 3.529E+2 1.904E+3
ratioG 1.084E-1 6.144E-2 3.480E-1 2.136E-1
ratioG_corr 4.870E-3 4.334E-2 8.544E-2 1.253E-1
f_1 8.469E-1 1.792E-1 3.372E-1 1.865E-1 8.551E-1 1.694E-1 8.774E-1 1.159E-1 4.771E-1 2.723E-1 8.699E-1 1.274E-1
tauD_1 [ms] 1.061E+1 5.819E+0 1.860E+0 4.441E-16 7.485E+0 4.249E+0 3.088E+0 4.536E+0 1.860E+0 0.000E+0 3.842E+0 1.631E+0
D_1 [mum^2/s] 2.255E+0 9.384E+0 6.926E+0 8.882E-16 2.217E+0 1.471E+0 1.557E+1 1.965E+1 6.926E+0 8.882E-16 3.798E+0 1.965E+0
alpha_1 9.752E-1 1.008E-1 9.200E-1 3.331E-16 9.242E-1 1.341E-1 9.387E-1 1.410E-1 9.200E-1 1.110E-16 9.100E-1 1.597E-1
f_2 1.531E-1 1.792E-1 6.628E-1 1.865E-1 1.449E-1 1.694E-1 1.226E-1 1.159E-1 5.229E-1 2.723E-1 1.301E-1 1.274E-1
tauD_2 [ms] 8.196E+1 4.273E+1 1.054E+2 9.038E+1 1.130E+2 9.563E+1 4.228E+1 4.407E+1 6.473E+1 9.739E+1 6.779E+1 5.701E+1
D_2 [mum^2/s] 1.631E-1 8.422E-2 2.658E-1 4.035E-1 1.579E-1 9.555E-2 4.459E-1 3.499E-1 9.150E-1 1.167E+0 2.384E-1 1.037E-1
alpha_2 1.396E+0 3.334E-1 1.232E+0 3.042E-1 1.472E+0 4.197E-1 1.259E+0 2.848E-1 1.252E+0 3.368E-1 1.518E+0 3.815E-1
tauD_m [ms] 2.028E+1 1.265E+1 6.916E+1 6.246E+1 2.267E+1 3.597E+1 9.609E+0 2.071E+1 2.794E+1 5.410E+1 1.288E+1 2.395E+1
D_m [mum^2/s] 1.427E+0 4.740E+0 2.527E+0 1.219E+0 1.786E+0 9.662E-1 1.393E+1 1.791E+1 3.991E+0 1.357E+0 3.227E+0 1.355E+0
R [1/s] 2.710E+0 5.186E+0 4.369E+0 9.767E+0
CPM [kHz] 4.049E+0 2.242E+0 4.710E-1 3.103E-1 1.454E+0 1.778E+0 2.962E-1 2.014E-1
CPM_corr [kHz] 4.098E+0 2.251E+0 5.602E-1 3.718E-1 1.685E+0 1.779E+0 9.078E-1 1.098E+0
Int [kHz] 1.815E+2 1.207E+2 1.682E+2 5.569E+1 2.993E+1 5.200E+1 4.114E+1 3.904E+1
Int_corr [kHz] 2.167E+2 1.553E+2 2.668E+2 9.810E+1 4.104E+1 1.091E+2 6.174E+1 6.892E+1
# samples 203 203 187 187 154 154 167 167 50 50 45 45
# cells 91

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 523.464 - sd = 807.429Int_corr [kHz] - Ch1 - Nuc : ave = 216.728 - sd = 155.288r = 0.401 - r^2 = 0.160
f_1 - Ch1 - Nuc : ave = 0.847 - sd = 0.179D_1 [mum^2/s] - Ch1 - Nuc : ave = 2.255 - sd = 9.384r = -0.660 - r^2 = 0.435
f_2 - Ch1 - Nuc : ave = 0.153 - sd = 0.179D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.163 - sd = 0.084r = 0.115 - r^2 = 0.013
CPM_corr [kHz] - Ch1 - Nuc : ave = 4.098 - sd = 2.251D_m [mum^2/s] - Ch1 - Nuc : ave = 1.427 - sd = 4.740r = -0.426 - r^2 = 0.182
c_corr [nM] - Ch1 - Nuc : ave = 523.464 - sd = 807.429ratioG_corr - Cross - Nuc : ave = 0.005 - sd = 0.043r = 0.300 - r^2 = 0.090
c_corr [nM] - Ch1 - Cyto : ave = 249.199 - sd = 656.121Int_corr [kHz] - Ch1 - Cyto : ave = 41.036 - sd = 109.143r = 0.226 - r^2 = 0.051
f_1 - Ch1 - Cyto : ave = 0.877 - sd = 0.116D_1 [mum^2/s] - Ch1 - Cyto : ave = 15.567 - sd = 19.653r = -0.071 - r^2 = 0.005
f_2 - Ch1 - Cyto : ave = 0.123 - sd = 0.116D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.446 - sd = 0.350r = 0.144 - r^2 = 0.021
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.685 - sd = 1.779D_m [mum^2/s] - Ch1 - Cyto : ave = 13.931 - sd = 17.912r = -0.537 - r^2 = 0.289