Automated F(C)CS analysis of clone # 1376

Created on 12/08/2014
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Gene and protein information

Clone # 1376
Gene symbol: RPL36
Description: ribosomal protein L36
Molecular weight (with FP): 39.3 kDa
Localisation: homogeneous nucleus, bright nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
1376_img_ch1.png1376_img_ch2.png


Point 1, intensity traces and correlation functions
1376_itr_1.png1376_cor_1.png

Point 2, intensity traces and correlation functions
1376_itr_2.png1376_cor_2.png

Point 3, intensity traces and correlation functions
1376_itr_3.png1376_cor_3.png

Point 4, intensity traces and correlation functions
1376_itr_4.png1376_cor_4.png

Point 5, intensity traces and correlation functions
1376_itr_5.png1376_cor_5.png

Point 6, intensity traces and correlation functions
1376_itr_6.png1376_cor_6.png

Sessions

1 20110902\U01--V00--1376
2 20110906\U03--V00--1376
3 20110907\U02--V00--1376
4 20110908\U02--V00--1376

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.523E+2 2.138E+2 9.845E+2 4.637E+2 2.807E+1 4.962E+1 4.242E+1 3.551E+1 2.107E+2 1.700E+2 2.639E+1 2.399E+1
N_corr 2.043E+2 2.889E+2 1.470E+3 7.711E+2 3.736E+1 7.118E+1 5.533E+1 5.188E+1 2.473E+2 3.544E+2 5.000E+1 4.947E+1
c [nM] 9.474E+2 1.329E+3 4.567E+3 2.151E+3 1.443E+2 2.551E+2 2.638E+2 2.208E+2 9.775E+2 7.883E+2 1.357E+2 1.233E+2
c_corr [nM] 1.271E+3 1.797E+3 6.820E+3 3.576E+3 1.921E+2 3.660E+2 3.441E+2 3.226E+2 1.147E+3 1.644E+3 2.570E+2 2.543E+2
ratioG 1.273E-1 9.184E-2 4.673E-1 2.587E-1
ratioG_corr 2.320E-2 3.606E-2 9.731E-2 8.469E-2
f_1 7.963E-1 1.314E-1 3.914E-1 1.940E-1 9.627E-1 8.851E-2 7.749E-1 9.634E-2 1.000E+0 0.000E+0 8.660E-1 1.123E-1
tauD_1 [ms] 2.253E+0 2.390E+0 1.905E+0 9.987E-2 1.025E+0 7.300E-1 2.070E+0 1.365E+0 6.811E+0 1.062E+1 1.735E+0 2.567E+0
D_1 [mum^2/s] 7.781E+0 4.713E+0 6.781E+0 3.574E-1 2.285E+1 2.428E+1 8.159E+0 6.784E+0 7.069E+0 1.692E+1 1.515E+1 1.735E+1
alpha_1 9.711E-1 1.907E-1 9.305E-1 9.987E-3 7.920E-1 2.084E-1 9.107E-1 1.814E-1 8.045E-1 2.375E-1 7.375E-1 2.229E-1
f_2 2.037E-1 1.314E-1 6.086E-1 1.940E-1 3.727E-2 8.851E-2 2.251E-1 9.634E-2 0.000E+0 0.000E+0 1.340E-1 1.123E-1
tauD_2 [ms] 3.853E+1 3.927E+1 1.129E+2 1.057E+2 7.370E+1 6.807E+1 4.924E+1 3.815E+1 5.000E+0 0.000E+0 6.970E+1 4.457E+1
D_2 [mum^2/s] 4.409E-1 2.348E-1 3.129E-1 4.274E-1 3.047E-1 3.902E-1 3.160E-1 1.998E-1 2.576E+0 4.441E-16 2.238E-1 1.091E-1
alpha_2 1.448E+0 3.347E-1 1.330E+0 3.139E-1 1.145E+0 2.319E-1 1.396E+0 3.345E-1 1.089E+0 6.298E-2 1.421E+0 3.461E-1
tauD_m [ms] 1.083E+1 1.309E+1 7.242E+1 7.975E+1 6.192E+0 2.349E+1 1.234E+1 1.153E+1 6.811E+0 1.062E+1 1.195E+1 1.720E+1
D_m [mum^2/s] 6.413E+0 4.044E+0 2.843E+0 1.185E+0 2.227E+1 2.427E+1 6.481E+0 5.726E+0 7.069E+0 1.692E+1 1.248E+1 1.281E+1
R [1/s] 3.867E+1 2.593E+1 0.000E+0 0.000E+0
CPM [kHz] 1.229E+0 3.785E-1 3.921E-1 3.420E-1 9.715E-1 4.611E-1 2.176E-1 1.977E-1
CPM_corr [kHz] 1.274E+0 3.670E-1 4.492E-1 3.755E-1 1.067E+0 4.614E-1 6.901E-1 4.290E-1
Int [kHz] 1.775E+2 2.306E+2 1.346E+2 3.467E+1 4.153E+1 3.649E+1 3.565E+1 3.171E+1
Int_corr [kHz] 2.311E+2 2.910E+2 2.291E+2 6.524E+1 5.696E+1 5.077E+1 5.898E+1 6.368E+1
# samples 204 204 99 99 63 63 366 366 61 61 54 54
# cells 107

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 1270.771 - sd = 1796.672Int_corr [kHz] - Ch1 - Nuc : ave = 231.106 - sd = 291.000r = 0.913 - r^2 = 0.833
f_1 - Ch1 - Nuc : ave = 0.796 - sd = 0.131D_1 [mum^2/s] - Ch1 - Nuc : ave = 7.781 - sd = 4.713r = 0.247 - r^2 = 0.061
f_2 - Ch1 - Nuc : ave = 0.204 - sd = 0.131D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.441 - sd = 0.235r = -0.354 - r^2 = 0.125
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.274 - sd = 0.367D_m [mum^2/s] - Ch1 - Nuc : ave = 6.413 - sd = 4.044r = 0.047 - r^2 = 0.002
c_corr [nM] - Ch1 - Nuc : ave = 1270.771 - sd = 1796.672ratioG_corr - Cross - Nuc : ave = 0.023 - sd = 0.036r = 0.345 - r^2 = 0.119
c_corr [nM] - Ch1 - Cyto : ave = 344.134 - sd = 322.626Int_corr [kHz] - Ch1 - Cyto : ave = 56.957 - sd = 50.773r = 0.848 - r^2 = 0.720
f_1 - Ch1 - Cyto : ave = 0.775 - sd = 0.096D_1 [mum^2/s] - Ch1 - Cyto : ave = 8.159 - sd = 6.784r = 0.075 - r^2 = 0.006
f_2 - Ch1 - Cyto : ave = 0.225 - sd = 0.096D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.316 - sd = 0.200r = 0.123 - r^2 = 0.015
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.067 - sd = 0.461D_m [mum^2/s] - Ch1 - Cyto : ave = 6.481 - sd = 5.726r = -0.106 - r^2 = 0.011