Automated F(C)CS analysis of clone # 1291

Created on 12/08/2014
Back to overview

Gene and protein information

Clone # 1291
Gene symbol: DDX24
Description: DEAD (Asp-Glu-Ala-Asp) box polypeptide 24
Molecular weight (with FP): 123.3 kDa
Localisation: homogeneous nucleus, bright nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
1291_img_ch1.png1291_img_ch2.png


Point 1, intensity traces and correlation functions
1291_itr_1.png1291_cor_1.png

Point 2, intensity traces and correlation functions
1291_itr_2.png1291_cor_2.png

Point 3, intensity traces and correlation functions
1291_itr_3.png1291_cor_3.png

Point 4, intensity traces and correlation functions
1291_itr_4.png1291_cor_4.png

Point 5, intensity traces and correlation functions
1291_itr_5.png1291_cor_5.png

Point 6, intensity traces and correlation functions
1291_itr_6.png1291_cor_6.png

Sessions

1 20111111\U02--V00--1291
2 20111115\U02--V00--1291
3 20111116\U02--V00--1291
4 20111213\U01--V00--1291
5 20120117\U03--V00--1291
6 20120208\U01--V00--1291
7 20120214\U01--V00--1291
8 20120222\U01--V00--1291

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 4.528E+2 4.770E+2 1.252E+3 6.934E+2 8.122E+1 1.378E+2 2.304E+1 4.565E+1 2.341E+2 1.817E+2 1.042E+1 1.258E+1
N_corr 6.751E+2 8.647E+2 1.541E+3 9.041E+2 1.657E+2 3.237E+2 2.400E+1 5.243E+1 3.416E+2 3.286E+2 2.259E+1 5.281E+1
c [nM] 2.816E+3 2.967E+3 5.808E+3 3.216E+3 4.176E+2 7.082E+2 1.433E+2 2.839E+2 1.086E+3 8.430E+2 5.357E+1 6.467E+1
c_corr [nM] 4.198E+3 5.378E+3 7.147E+3 4.194E+3 8.517E+2 1.664E+3 1.492E+2 3.261E+2 1.584E+3 1.524E+3 1.161E+2 2.715E+2
ratioG 1.928E-1 1.647E-1 2.274E-1 1.107E-1
ratioG_corr 4.541E-2 1.573E-1 1.329E-1 1.698E-1
f_1 8.557E-1 1.261E-1 3.950E-1 2.255E-1 9.711E-1 6.856E-2 9.008E-1 5.706E-2 7.861E-1 1.965E-1 9.476E-1 5.348E-2
tauD_1 [ms] 3.015E+0 2.334E+0 1.875E+0 1.453E-1 1.857E+0 9.546E-1 5.193E-1 5.384E-1 1.860E+0 4.441E-16 1.345E+0 9.272E-1
D_1 [mum^2/s] 4.410E+0 1.786E+0 6.896E+0 3.477E-1 1.196E+1 1.687E+1 3.043E+1 1.565E+1 6.926E+0 0.000E+0 2.147E+1 2.065E+1
alpha_1 9.984E-1 1.262E-1 9.271E-1 4.352E-2 9.317E-1 2.424E-1 1.023E+0 1.165E-1 9.201E-1 5.151E-4 9.882E-1 2.445E-1
f_2 1.443E-1 1.261E-1 6.050E-1 2.255E-1 2.892E-2 6.856E-2 9.922E-2 5.706E-2 2.139E-1 1.965E-1 5.238E-2 5.348E-2
tauD_2 [ms] 5.429E+1 5.563E+1 6.927E+1 1.031E+2 8.375E+1 7.570E+1 1.630E+1 2.000E+1 1.457E+2 2.504E+2 4.850E+1 2.512E+1
D_2 [mum^2/s] 3.163E-1 1.746E-1 6.882E-1 8.548E-1 2.142E-1 1.895E-1 1.017E+0 5.004E-1 6.161E-1 1.064E+0 3.473E-1 2.417E-1
alpha_2 1.377E+0 3.516E-1 1.305E+0 3.443E-1 1.113E+0 1.407E-1 1.085E+0 1.288E-1 1.311E+0 3.767E-1 1.092E+0 1.388E-1
tauD_m [ms] 1.175E+1 1.525E+1 3.912E+1 5.829E+1 5.967E+0 1.713E+1 2.501E+0 4.964E+0 4.523E+1 9.044E+1 4.523E+0 4.564E+0
D_m [mum^2/s] 3.768E+0 1.321E+0 3.195E+0 1.308E+0 1.140E+1 1.480E+1 2.781E+1 1.545E+1 5.511E+0 1.361E+0 2.055E+1 1.989E+1
R [1/s] 9.717E+0 1.513E+1 6.458E+1 5.459E+1
CPM [kHz] 1.636E+0 7.409E-1 3.966E-1 4.172E-1 9.136E-1 4.191E-1 3.186E-1 2.903E-1
CPM_corr [kHz] 1.646E+0 7.416E-1 5.358E-1 5.435E-1 1.135E+0 4.757E-1 4.587E-1 3.551E-1
Int [kHz] 5.363E+2 4.242E+2 2.245E+2 7.392E+1 1.672E+1 2.045E+1 5.453E+1 4.774E+1
Int_corr [kHz] 7.587E+2 6.862E+2 3.706E+2 1.420E+2 1.860E+1 2.234E+1 1.035E+2 1.161E+2
# samples 163 163 121 121 107 107 136 136 14 14 13 13
# cells 62

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 4198.464 - sd = 5377.697Int_corr [kHz] - Ch1 - Nuc : ave = 758.665 - sd = 686.158r = 0.641 - r^2 = 0.411
f_1 - Ch1 - Nuc : ave = 0.856 - sd = 0.126D_1 [mum^2/s] - Ch1 - Nuc : ave = 4.410 - sd = 1.786r = -0.267 - r^2 = 0.071
f_2 - Ch1 - Nuc : ave = 0.144 - sd = 0.126D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.316 - sd = 0.175r = 0.187 - r^2 = 0.035
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.646 - sd = 0.742D_m [mum^2/s] - Ch1 - Nuc : ave = 3.768 - sd = 1.321r = -0.211 - r^2 = 0.045
c_corr [nM] - Ch1 - Nuc : ave = 4198.464 - sd = 5377.697ratioG_corr - Cross - Nuc : ave = 0.045 - sd = 0.157r = 0.396 - r^2 = 0.157
c_corr [nM] - Ch1 - Cyto : ave = 149.243 - sd = 326.101Int_corr [kHz] - Ch1 - Cyto : ave = 18.601 - sd = 22.341r = 0.659 - r^2 = 0.434
f_1 - Ch1 - Cyto : ave = 0.901 - sd = 0.057D_1 [mum^2/s] - Ch1 - Cyto : ave = 30.429 - sd = 15.645r = 0.321 - r^2 = 0.103
f_2 - Ch1 - Cyto : ave = 0.099 - sd = 0.057D_2 [mum^2/s] - Ch1 - Cyto : ave = 1.017 - sd = 0.500r = -0.235 - r^2 = 0.055
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.135 - sd = 0.476D_m [mum^2/s] - Ch1 - Cyto : ave = 27.812 - sd = 15.454r = 0.261 - r^2 = 0.068