Automated F(C)CS analysis of clone # 1115

Created on 12/08/2014
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Gene and protein information

Clone # 1115
Gene symbol: UTP3
Description: UTP3, small subunit (SSU) processome component, homolog (S. cerevisiae)
Molecular weight (with FP): 81.6 kDa
Localisation: homogeneous nucleus, bright nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
1115_img_ch1.png1115_img_ch2.png


Point 1, intensity traces and correlation functions
1115_itr_1.png1115_cor_1.png

Point 2, intensity traces and correlation functions
1115_itr_2.png1115_cor_2.png

Point 3, intensity traces and correlation functions
1115_itr_3.png1115_cor_3.png

Point 4, intensity traces and correlation functions
1115_itr_4.png1115_cor_4.png

Point 5, intensity traces and correlation functions
1115_itr_5.png1115_cor_5.png

Point 6, intensity traces and correlation functions
1115_itr_6.png1115_cor_6.png

Sessions

1 20111018\U03--V00--1115
2 20111108\U00--V01--1115
3 20111109\U00--V01--1115
4 20111110\U00--V01--1115
5 20111208\U02--V00--1115

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 3.748E+2 4.804E+2 1.200E+3 7.522E+2 4.931E+1 8.739E+1 4.066E+1 4.948E+1 3.188E+2 2.440E+2 4.034E+1 6.303E+1
N_corr 4.976E+2 6.819E+2 1.582E+3 1.056E+3 7.877E+1 1.505E+2 3.892E+1 4.980E+1 3.662E+2 4.132E+2 3.879E+1 5.300E+1
c [nM] 2.331E+3 2.988E+3 5.566E+3 3.489E+3 2.535E+2 4.493E+2 2.529E+2 3.077E+2 1.479E+3 1.132E+3 2.074E+2 3.240E+2
c_corr [nM] 3.095E+3 4.241E+3 7.339E+3 4.899E+3 4.049E+2 7.738E+2 2.420E+2 3.097E+2 1.699E+3 1.916E+3 1.994E+2 2.725E+2
ratioG 1.414E-1 1.090E-1 4.496E-1 2.207E-1
ratioG_corr 1.435E-2 6.450E-2 1.823E-1 1.936E-1
f_1 8.914E-1 1.511E-1 3.876E-1 2.064E-1 9.647E-1 7.688E-2 8.449E-1 1.052E-1 5.982E-1 2.914E-1 9.525E-1 8.980E-2
tauD_1 [ms] 3.063E+0 4.361E+0 1.860E+0 2.220E-16 1.883E+0 1.268E+0 1.245E+0 9.416E-1 1.860E+0 4.441E-16 1.878E+0 8.383E-1
D_1 [mum^2/s] 9.665E+0 1.238E+1 6.926E+0 8.882E-16 2.083E+1 3.308E+1 1.515E+1 1.176E+1 6.926E+0 8.882E-16 8.493E+0 5.362E+0
alpha_1 1.003E+0 1.253E-1 9.200E-1 2.220E-16 9.297E-1 2.383E-1 9.347E-1 1.373E-1 9.200E-1 0.000E+0 9.030E-1 2.465E-1
f_2 1.086E-1 1.511E-1 6.124E-1 2.064E-1 3.531E-2 7.688E-2 1.551E-1 1.052E-1 4.018E-1 2.914E-1 4.745E-2 8.980E-2
tauD_2 [ms] 4.618E+1 5.372E+1 7.288E+1 8.254E+1 8.959E+1 6.297E+1 3.286E+1 2.091E+1 2.457E+1 3.522E+1 4.369E+1 2.178E+1
D_2 [mum^2/s] 5.938E-1 6.295E-1 5.242E-1 6.691E-1 1.964E-1 1.150E-1 4.872E-1 3.874E-1 1.768E+0 1.364E+0 3.336E-1 1.385E-1
alpha_2 1.239E+0 3.160E-1 1.232E+0 2.603E-1 1.131E+0 1.692E-1 1.158E+0 2.128E-1 1.274E+0 3.977E-1 1.286E+0 3.835E-1
tauD_m [ms] 1.181E+1 2.103E+1 4.386E+1 4.958E+1 6.367E+0 1.444E+1 6.610E+0 5.506E+0 6.817E+0 7.561E+0 2.853E+0 1.575E+0
D_m [mum^2/s] 9.039E+0 1.217E+1 3.054E+0 1.257E+0 2.066E+1 3.316E+1 1.309E+1 1.080E+1 5.152E+0 1.036E+0 8.053E+0 5.315E+0
R [1/s] 4.337E+1 7.090E+1 4.480E+0 9.956E+0
CPM [kHz] 1.560E+0 6.405E-1 5.000E-1 4.188E-1 5.665E-1 5.770E-1 2.032E-1 1.073E-1
CPM_corr [kHz] 1.574E+0 6.384E-1 6.367E-1 5.370E-1 7.244E-1 5.758E-1 3.764E-1 2.204E-1
Int [kHz] 4.185E+2 3.937E+2 2.221E+2 5.565E+1 2.304E+1 3.019E+1 5.955E+1 4.816E+1
Int_corr [kHz] 5.404E+2 5.517E+2 3.630E+2 1.130E+2 2.531E+1 3.250E+1 8.104E+1 6.752E+1
# samples 94 94 76 76 65 65 74 74 11 11 8 8
# cells 37

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 3094.766 - sd = 4240.651Int_corr [kHz] - Ch1 - Nuc : ave = 540.414 - sd = 551.729r = 0.753 - r^2 = 0.567
f_1 - Ch1 - Nuc : ave = 0.891 - sd = 0.151D_1 [mum^2/s] - Ch1 - Nuc : ave = 9.665 - sd = 12.378r = 0.046 - r^2 = 0.002
f_2 - Ch1 - Nuc : ave = 0.109 - sd = 0.151D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.594 - sd = 0.630r = 0.029 - r^2 = 0.001
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.574 - sd = 0.638D_m [mum^2/s] - Ch1 - Nuc : ave = 9.039 - sd = 12.174r = -0.054 - r^2 = 0.003
c_corr [nM] - Ch1 - Nuc : ave = 3094.766 - sd = 4240.651ratioG_corr - Cross - Nuc : ave = 0.014 - sd = 0.065r = -0.274 - r^2 = 0.075
c_corr [nM] - Ch1 - Cyto : ave = 242.048 - sd = 309.702Int_corr [kHz] - Ch1 - Cyto : ave = 25.311 - sd = 32.498r = 0.661 - r^2 = 0.437
f_1 - Ch1 - Cyto : ave = 0.845 - sd = 0.105D_1 [mum^2/s] - Ch1 - Cyto : ave = 15.151 - sd = 11.763r = 0.171 - r^2 = 0.029
f_2 - Ch1 - Cyto : ave = 0.155 - sd = 0.105D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.487 - sd = 0.387r = -0.404 - r^2 = 0.163
CPM_corr [kHz] - Ch1 - Cyto : ave = 0.724 - sd = 0.576D_m [mum^2/s] - Ch1 - Cyto : ave = 13.089 - sd = 10.803r = -0.146 - r^2 = 0.021