Automated F(C)CS analysis of clone # 1099

Created on 12/08/2014
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Gene and protein information

Clone # 1099
Gene symbol: ZFP82
Description: zinc finger protein 82 homolog
Molecular weight (with FP): 89.5 kDa
Localisation: granular nucleus, ave. nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
1099_img_ch1.png1099_img_ch2.png


Point 1, intensity traces and correlation functions
1099_itr_1.png1099_cor_1.png

Point 2, intensity traces and correlation functions
1099_itr_2.png1099_cor_2.png

Point 3, intensity traces and correlation functions
1099_itr_3.png1099_cor_3.png

Point 4, intensity traces and correlation functions
1099_itr_4.png1099_cor_4.png

Point 5, intensity traces and correlation functions
1099_itr_5.png1099_cor_5.png

Point 6, intensity traces and correlation functions
1099_itr_6.png1099_cor_6.png

Sessions

1 20111104\U01--V00--1099
2 20111108\U03--V00--1099
3 20111109\U01--V01--1099
4 20111110\U03--V00--1099

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.192E+2 5.681E+1 1.390E+3 5.735E+2 9.059E+0 7.749E+0 2.280E+1 2.336E+1 3.620E+2 3.968E+2 9.283E+0 6.801E+0
N_corr 1.321E+2 6.535E+1 2.005E+3 9.459E+2 1.083E+1 9.197E+0 2.379E+1 2.985E+1 5.543E+2 7.077E+2 1.380E+1 1.505E+1
c [nM] 7.416E+2 3.533E+2 6.446E+3 2.660E+3 4.657E+1 3.984E+1 1.418E+2 1.453E+2 1.679E+3 1.840E+3 4.772E+1 3.496E+1
c_corr [nM] 8.213E+2 4.064E+2 9.301E+3 4.387E+3 5.569E+1 4.728E+1 1.480E+2 1.856E+2 2.571E+3 3.283E+3 7.097E+1 7.738E+1
ratioG 7.280E-2 3.816E-2 2.252E-1 1.731E-1
ratioG_corr 9.092E-3 3.238E-2 1.100E-1 9.701E-2
f_1 8.350E-1 1.247E-1 3.663E-1 1.967E-1 9.441E-1 9.932E-2 9.414E-1 5.297E-2 5.736E-1 3.243E-1 9.174E-1 1.538E-1
tauD_1 [ms] 3.447E+0 1.933E+0 1.860E+0 4.441E-16 3.010E+0 4.181E+0 4.042E-1 4.986E-1 1.860E+0 2.220E-16 1.612E+0 1.129E+0
D_1 [mum^2/s] 4.612E+0 3.808E+0 6.926E+0 6.217E-15 1.140E+1 1.888E+1 3.729E+1 1.542E+1 6.926E+0 0.000E+0 2.445E+1 2.740E+1
alpha_1 9.762E-1 1.007E-1 9.200E-1 1.221E-15 8.240E-1 2.296E-1 1.025E+0 1.071E-1 9.200E-1 1.110E-16 9.042E-1 2.259E-1
f_2 1.650E-1 1.247E-1 6.337E-1 1.967E-1 5.587E-2 9.932E-2 5.856E-2 5.297E-2 4.264E-1 3.243E-1 8.261E-2 1.538E-1
tauD_2 [ms] 3.800E+1 2.490E+1 7.869E+1 1.010E+2 1.507E+2 3.333E+2 1.334E+1 1.828E+1 1.052E+2 1.451E+2 1.129E+2 1.252E+2
D_2 [mum^2/s] 3.614E-1 1.737E-1 5.122E-1 6.168E-1 1.342E-1 7.799E-2 1.389E+0 6.984E-1 8.508E-1 1.371E+0 3.948E-1 5.018E-1
alpha_2 1.392E+0 3.523E-1 1.306E+0 3.194E-1 1.187E+0 2.614E-1 1.014E+0 1.247E-1 1.463E+0 4.094E-1 1.218E+0 2.985E-1
tauD_m [ms] 9.079E+0 7.315E+0 4.968E+1 6.471E+1 1.375E+1 3.538E+1 1.449E+0 3.146E+0 4.675E+1 6.890E+1 2.535E+1 5.624E+1
D_m [mum^2/s] 3.755E+0 2.964E+0 2.888E+0 1.221E+0 1.061E+1 1.716E+1 3.543E+1 1.533E+1 4.576E+0 1.709E+0 2.249E+1 2.615E+1
R [1/s] 1.267E+1 1.492E+1 6.472E+1 7.309E+1
CPM [kHz] 1.468E+0 6.223E-1 4.269E-1 4.074E-1 8.524E-1 4.208E-1 4.258E-1 4.256E-1
CPM_corr [kHz] 1.488E+0 6.239E-1 4.754E-1 4.521E-1 1.028E+0 4.524E-1 6.590E-1 5.267E-1
Int [kHz] 1.762E+2 9.236E+1 2.755E+2 7.685E+1 2.015E+1 2.501E+1 1.016E+2 1.040E+2
Int_corr [kHz] 1.956E+2 1.015E+2 4.345E+2 1.328E+2 2.270E+1 2.835E+1 1.705E+2 1.907E+2
# samples 521 521 441 441 373 373 364 364 29 29 19 19
# cells 191

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 821.289 - sd = 406.446Int_corr [kHz] - Ch1 - Nuc : ave = 195.578 - sd = 101.469r = 0.688 - r^2 = 0.473
f_1 - Ch1 - Nuc : ave = 0.835 - sd = 0.125D_1 [mum^2/s] - Ch1 - Nuc : ave = 4.612 - sd = 3.808r = -0.430 - r^2 = 0.185
f_2 - Ch1 - Nuc : ave = 0.165 - sd = 0.125D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.361 - sd = 0.174r = 0.278 - r^2 = 0.077
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.488 - sd = 0.624D_m [mum^2/s] - Ch1 - Nuc : ave = 3.755 - sd = 2.964r = -0.236 - r^2 = 0.056
c_corr [nM] - Ch1 - Nuc : ave = 821.289 - sd = 406.446ratioG_corr - Cross - Nuc : ave = 0.009 - sd = 0.032r = 0.251 - r^2 = 0.063
c_corr [nM] - Ch1 - Cyto : ave = 147.950 - sd = 185.628Int_corr [kHz] - Ch1 - Cyto : ave = 22.697 - sd = 28.354r = 0.712 - r^2 = 0.508
f_1 - Ch1 - Cyto : ave = 0.941 - sd = 0.053D_1 [mum^2/s] - Ch1 - Cyto : ave = 37.293 - sd = 15.424r = 0.161 - r^2 = 0.026
f_2 - Ch1 - Cyto : ave = 0.059 - sd = 0.053D_2 [mum^2/s] - Ch1 - Cyto : ave = 1.389 - sd = 0.698r = -0.033 - r^2 = 0.001
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.028 - sd = 0.452D_m [mum^2/s] - Ch1 - Cyto : ave = 35.431 - sd = 15.331r = 0.308 - r^2 = 0.095