Automated F(C)CS analysis of clone # 0921

Created on 12/08/2014
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Gene and protein information

Clone # 0921
Gene symbol: LHX6
Description: LIM homeobox 6
Molecular weight (with FP): 70.1 kDa
Localisation: granular nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
0921_img_ch1.png0921_img_ch2.png


Point 1, intensity traces and correlation functions
0921_itr_1.png0921_cor_1.png

Point 2, intensity traces and correlation functions
0921_itr_2.png0921_cor_2.png

Point 3, intensity traces and correlation functions
0921_itr_3.png0921_cor_3.png

Point 4, intensity traces and correlation functions
0921_itr_4.png0921_cor_4.png

Point 5, intensity traces and correlation functions
0921_itr_5.png0921_cor_5.png

Point 6, intensity traces and correlation functions
0921_itr_6.png0921_cor_6.png

Sessions

1 20110915\U00--V01--0921
2 20110916\U00--V01--0921
3 20110922\U03--V00--0921
4 20110928\U01--V01--0921
5 20110930\U00--V01--0921
6 20111011\U03--V00--0921

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.409E+2 1.251E+2 1.002E+3 4.784E+2 2.846E+1 4.196E+1 2.766E+1 9.455E+1 4.054E+2 3.623E+2 5.450E+0 9.650E+0
N_corr 1.550E+2 1.376E+2 1.303E+3 6.644E+2 3.586E+1 5.294E+1 2.934E+1 1.146E+2 5.667E+2 5.505E+2 7.603E+0 1.372E+1
c [nM] 8.762E+2 7.778E+2 4.646E+3 2.219E+3 1.463E+2 2.157E+2 1.720E+2 5.880E+2 1.880E+3 1.680E+3 2.802E+1 4.961E+1
c_corr [nM] 9.641E+2 8.556E+2 6.042E+3 3.082E+3 1.844E+2 2.721E+2 1.825E+2 7.126E+2 2.629E+3 2.553E+3 3.909E+1 7.052E+1
ratioG 1.360E-1 8.945E-2 1.265E-1 1.381E-1
ratioG_corr 3.442E-2 5.486E-2 2.679E-2 2.972E-2
f_1 8.383E-1 1.049E-1 4.621E-1 2.284E-1 9.643E-1 7.881E-2 8.923E-1 6.635E-2 7.819E-1 2.897E-1 1.000E+0 0.000E+0
tauD_1 [ms] 2.004E+0 8.576E-1 1.864E+0 5.655E-2 1.476E+0 1.078E+0 8.488E-1 5.534E-1 6.282E+0 1.181E+1 7.275E-1 3.599E-1
D_1 [mum^2/s] 6.214E+0 2.869E+0 6.916E+0 2.017E-1 1.634E+1 2.020E+1 1.626E+1 7.337E+0 6.150E+0 3.640E+0 2.738E+1 3.029E+1
alpha_1 9.257E-1 1.078E-1 9.299E-1 2.635E-2 7.501E-1 1.941E-1 9.409E-1 1.206E-1 8.601E-1 1.439E-1 7.390E-1 2.327E-1
f_2 1.617E-1 1.049E-1 5.379E-1 2.284E-1 3.572E-2 7.881E-2 1.077E-1 6.635E-2 2.181E-1 2.897E-1 0.000E+0 0.000E+0
tauD_2 [ms] 3.000E+1 1.472E+1 7.952E+1 9.072E+1 8.076E+1 8.566E+1 2.196E+1 3.200E+1 8.463E+1 1.395E+2 8.549E+1 7.798E+1
D_2 [mum^2/s] 4.086E-1 1.331E-1 5.368E-1 7.589E-1 2.143E-1 1.001E-1 6.714E-1 2.707E-1 1.473E+0 1.135E+0 2.422E-1 1.380E-1
alpha_2 1.423E+0 3.047E-1 1.300E+0 3.434E-1 1.163E+0 2.043E-1 1.122E+0 1.524E-1 1.218E+0 2.605E-1 1.076E+0 1.387E-2
tauD_m [ms] 6.183E+0 3.323E+0 4.550E+1 6.218E+1 7.677E+0 3.261E+1 3.818E+0 1.076E+1 4.224E+1 6.703E+1 7.275E-1 3.599E-1
D_m [mum^2/s] 5.257E+0 2.468E+0 3.532E+0 1.390E+0 1.586E+1 2.013E+1 1.456E+1 6.550E+0 4.746E+0 3.788E+0 2.738E+1 3.029E+1
R [1/s] 2.075E+1 1.631E+1 2.117E+1 3.198E+1
CPM [kHz] 1.325E+0 3.554E-1 2.777E-1 2.035E-1 8.255E-1 4.074E-1 3.375E-1 2.928E-1
CPM_corr [kHz] 1.349E+0 3.600E-1 3.345E-1 2.391E-1 1.057E+0 4.588E-1 4.511E-1 3.623E-1
Int [kHz] 1.881E+2 1.592E+2 1.533E+2 4.298E+1 1.792E+1 3.441E+1 7.221E+1 4.814E+1
Int_corr [kHz] 2.077E+2 1.753E+2 2.393E+2 7.737E+1 2.048E+1 3.975E+1 1.140E+2 8.421E+1
# samples 138 138 105 105 90 90 118 118 17 17 11 11
# cells 63

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 964.066 - sd = 855.590Int_corr [kHz] - Ch1 - Nuc : ave = 207.706 - sd = 175.296r = 0.733 - r^2 = 0.537
f_1 - Ch1 - Nuc : ave = 0.838 - sd = 0.105D_1 [mum^2/s] - Ch1 - Nuc : ave = 6.214 - sd = 2.869r = -0.186 - r^2 = 0.035
f_2 - Ch1 - Nuc : ave = 0.162 - sd = 0.105D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.409 - sd = 0.133r = 0.178 - r^2 = 0.032
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.349 - sd = 0.360D_m [mum^2/s] - Ch1 - Nuc : ave = 5.257 - sd = 2.468r = 0.168 - r^2 = 0.028
c_corr [nM] - Ch1 - Nuc : ave = 964.066 - sd = 855.590ratioG_corr - Cross - Nuc : ave = 0.034 - sd = 0.055r = 0.247 - r^2 = 0.061
c_corr [nM] - Ch1 - Cyto : ave = 182.482 - sd = 712.641Int_corr [kHz] - Ch1 - Cyto : ave = 20.484 - sd = 39.746r = 0.677 - r^2 = 0.459
f_1 - Ch1 - Cyto : ave = 0.892 - sd = 0.066D_1 [mum^2/s] - Ch1 - Cyto : ave = 16.256 - sd = 7.337r = -0.158 - r^2 = 0.025
f_2 - Ch1 - Cyto : ave = 0.108 - sd = 0.066D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.671 - sd = 0.271r = 0.017 - r^2 = 0.000
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.057 - sd = 0.459D_m [mum^2/s] - Ch1 - Cyto : ave = 14.557 - sd = 6.550r = 0.283 - r^2 = 0.080