Automated F(C)CS analysis of clone # 0775

Created on 12/08/2014
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Gene and protein information

Clone # 0775
Gene symbol: HORMAD1
Description: HORMA domain containing 1
Molecular weight (with FP): 72.2 kDa
Localisation: granular nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
0775_img_ch1.png0775_img_ch2.png


Point 1, intensity traces and correlation functions
0775_itr_1.png0775_cor_1.png

Point 2, intensity traces and correlation functions
0775_itr_2.png0775_cor_2.png

Point 3, intensity traces and correlation functions
0775_itr_3.png0775_cor_3.png

Point 4, intensity traces and correlation functions
0775_itr_4.png0775_cor_4.png

Point 5, intensity traces and correlation functions
0775_itr_5.png0775_cor_5.png

Point 6, intensity traces and correlation functions
0775_itr_6.png0775_cor_6.png

Sessions

1 20110915\U02--V00--0775
2 20110916\U02--V00--0775
3 20110922\U02--V00--0775
4 20110930\U03--V00--0775
5 20111011\U02--V00--0775

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 9.892E+1 5.869E+1 1.145E+3 4.964E+2 9.760E+0 1.314E+1 1.877E+1 1.721E+1 4.273E+2 3.998E+2 9.319E+0 6.753E+0
N_corr 1.086E+2 6.385E+1 1.660E+3 7.606E+2 1.308E+1 1.973E+1 2.088E+1 2.540E+1 7.332E+2 8.209E+2 1.451E+1 1.183E+1
c [nM] 6.152E+2 3.650E+2 5.313E+3 2.302E+3 5.018E+1 6.754E+1 1.167E+2 1.070E+2 1.982E+3 1.854E+3 4.791E+1 3.472E+1
c_corr [nM] 6.755E+2 3.971E+2 7.700E+3 3.528E+3 6.725E+1 1.014E+2 1.299E+2 1.580E+2 3.401E+3 3.807E+3 7.461E+1 6.083E+1
ratioG 6.888E-2 4.797E-2 2.858E-1 1.833E-1
ratioG_corr 1.092E-2 4.476E-2 7.924E-2 7.685E-2
f_1 8.947E-1 5.249E-2 4.194E-1 2.215E-1 9.772E-1 4.755E-2 9.013E-1 5.813E-2 7.387E-1 3.698E-1 9.450E-1 5.507E-2
tauD_1 [ms] 1.351E+0 5.593E-1 1.834E+0 2.979E-2 1.260E+0 1.194E+0 6.393E-1 5.561E-1 1.850E+0 2.236E-2 1.038E+0 7.900E-1
D_1 [mum^2/s] 9.641E+0 5.198E+0 7.028E+0 1.146E-1 2.498E+1 3.066E+1 2.559E+1 1.444E+1 6.964E+0 8.604E-2 2.987E+1 2.620E+1
alpha_1 9.466E-1 8.022E-2 9.200E-1 0.000E+0 8.660E-1 2.444E-1 9.739E-1 1.084E-1 9.200E-1 0.000E+0 1.017E+0 1.982E-1
f_2 1.053E-1 5.249E-2 5.806E-1 2.215E-1 2.285E-2 4.755E-2 9.873E-2 5.813E-2 2.613E-1 3.698E-1 5.500E-2 5.507E-2
tauD_2 [ms] 2.161E+1 1.136E+1 6.645E+1 1.001E+2 9.558E+1 7.909E+1 1.575E+1 1.256E+1 4.817E+1 4.840E+1 3.998E+1 2.393E+1
D_2 [mum^2/s] 5.866E-1 2.262E-1 6.950E-1 8.243E-1 1.775E-1 9.595E-2 9.889E-1 5.539E-1 7.420E-1 7.661E-1 5.305E-1 4.404E-1
alpha_2 1.294E+0 2.868E-1 1.323E+0 3.280E-1 1.116E+0 1.246E-1 1.102E+0 1.332E-1 1.214E+0 1.974E-1 9.525E-1 2.628E-1
tauD_m [ms] 3.528E+0 2.361E+0 3.460E+1 5.145E+1 4.733E+0 1.108E+1 2.295E+0 2.213E+0 8.251E+0 7.998E+0 4.373E+0 4.234E+0
D_m [mum^2/s] 8.634E+0 4.568E+0 3.449E+0 1.201E+0 2.469E+1 3.069E+1 2.329E+1 1.305E+1 5.527E+0 1.809E+0 2.952E+1 2.651E+1
R [1/s] 2.924E+1 2.967E+1 5.866E+1 4.744E+1
CPM [kHz] 1.557E+0 4.541E-1 3.190E-1 2.419E-1 1.215E+0 4.975E-1 2.679E-1 1.610E-1
CPM_corr [kHz] 1.589E+0 4.516E-1 3.534E-1 2.631E-1 1.412E+0 4.820E-1 4.108E-1 1.374E-1
Int [kHz] 1.671E+2 1.013E+2 2.165E+2 6.981E+1 2.687E+1 2.745E+1 9.833E+1 9.683E+1
Int_corr [kHz] 1.843E+2 1.083E+2 3.454E+2 1.192E+2 3.130E+1 3.591E+1 1.982E+2 2.259E+2
# samples 190 190 77 77 62 62 177 177 6 6 4 4
# cells 79

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 675.492 - sd = 397.101Int_corr [kHz] - Ch1 - Nuc : ave = 184.275 - sd = 108.300r = 0.893 - r^2 = 0.798
f_1 - Ch1 - Nuc : ave = 0.895 - sd = 0.052D_1 [mum^2/s] - Ch1 - Nuc : ave = 9.641 - sd = 5.198r = -0.114 - r^2 = 0.013
f_2 - Ch1 - Nuc : ave = 0.105 - sd = 0.052D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.587 - sd = 0.226r = -0.003 - r^2 = 8.554E-6
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.589 - sd = 0.452D_m [mum^2/s] - Ch1 - Nuc : ave = 8.634 - sd = 4.568r = 0.015 - r^2 = 0.000
c_corr [nM] - Ch1 - Nuc : ave = 675.492 - sd = 397.101ratioG_corr - Cross - Nuc : ave = 0.011 - sd = 0.045r = 0.668 - r^2 = 0.446
c_corr [nM] - Ch1 - Cyto : ave = 129.856 - sd = 157.979Int_corr [kHz] - Ch1 - Cyto : ave = 31.304 - sd = 35.905r = 0.910 - r^2 = 0.828
f_1 - Ch1 - Cyto : ave = 0.901 - sd = 0.058D_1 [mum^2/s] - Ch1 - Cyto : ave = 25.590 - sd = 14.444r = 0.451 - r^2 = 0.203
f_2 - Ch1 - Cyto : ave = 0.099 - sd = 0.058D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.989 - sd = 0.554r = -0.380 - r^2 = 0.144
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.412 - sd = 0.482D_m [mum^2/s] - Ch1 - Cyto : ave = 23.288 - sd = 13.050r = 0.253 - r^2 = 0.064