Automated F(C)CS analysis of clone # 0739

Created on 12/08/2014
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Gene and protein information

Clone # 0739
Gene symbol: SYF2
Description: SYF2 homolog, RNA splicing factor (S. cerevisiae)
Molecular weight (with FP): 55.7 kDa
Localisation: homogeneous nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
0739_img_ch1.png0739_img_ch2.png


Point 1, intensity traces and correlation functions
0739_itr_1.png0739_cor_1.png

Point 2, intensity traces and correlation functions
0739_itr_2.png0739_cor_2.png

Point 3, intensity traces and correlation functions
0739_itr_3.png0739_cor_3.png

Point 4, intensity traces and correlation functions
0739_itr_4.png0739_cor_4.png

Point 5, intensity traces and correlation functions
0739_itr_5.png0739_cor_5.png

Point 6, intensity traces and correlation functions
0739_itr_6.png0739_cor_6.png

Sessions

1 20110907\U01--V00--0739
2 20110908\U01--V00--0739
3 20110909\U01--V00--0739

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 2.923E+2 3.275E+2 1.087E+3 4.501E+2 4.729E+1 9.884E+1 2.824E+1 4.777E+1 4.305E+2 3.293E+2 2.879E+1 2.652E+1
N_corr 3.064E+2 3.412E+2 1.459E+3 7.418E+2 5.253E+1 1.103E+2 2.892E+1 6.723E+1 7.175E+2 7.888E+2 1.036E+2 1.984E+2
c [nM] 1.818E+3 2.037E+3 5.041E+3 2.088E+3 2.431E+2 5.081E+2 1.757E+2 2.971E+2 1.997E+3 1.528E+3 1.480E+2 1.364E+2
c_corr [nM] 1.905E+3 2.122E+3 6.766E+3 3.441E+3 2.700E+2 5.670E+2 1.799E+2 4.181E+2 3.328E+3 3.659E+3 5.328E+2 1.020E+3
ratioG 1.633E-1 1.237E-1 2.765E-1 1.870E-1
ratioG_corr 3.159E-2 4.304E-2 1.463E-1 2.871E-1
f_1 8.591E-1 1.626E-1 5.379E-1 2.114E-1 9.778E-1 5.021E-2 8.842E-1 1.013E-1 8.865E-1 1.500E-1 9.693E-1 6.207E-2
tauD_1 [ms] 1.302E+0 7.815E-1 1.880E+0 3.222E-2 7.758E-1 4.096E-1 8.911E-1 8.235E-1 4.767E+0 1.098E+1 1.372E+0 4.769E-1
D_1 [mum^2/s] 1.107E+1 6.037E+0 6.854E+0 1.207E-1 2.047E+1 1.282E+1 1.750E+1 2.119E+1 6.136E+0 1.978E+0 1.050E+1 5.423E+0
alpha_1 9.898E-1 1.599E-1 9.728E-1 3.791E-2 8.550E-1 1.692E-1 1.043E+0 1.901E-1 1.093E+0 1.751E-1 1.069E+0 1.823E-1
f_2 1.409E-1 1.626E-1 4.621E-1 2.114E-1 2.220E-2 5.021E-2 1.158E-1 1.013E-1 1.135E-1 1.500E-1 3.074E-2 6.207E-2
tauD_2 [ms] 1.546E+1 8.169E+0 9.012E+1 7.581E+1 4.150E+1 2.128E+1 1.675E+1 3.485E+1 8.606E+1 1.451E+2 4.584E+1 2.990E+1
D_2 [mum^2/s] 9.214E-1 5.489E-1 3.352E-1 3.907E-1 4.108E-1 3.426E-1 1.038E+0 5.973E-1 9.066E-1 1.042E+0 3.371E-1 1.400E-1
alpha_2 1.204E+0 2.877E-1 1.311E+0 2.308E-1 1.127E+0 1.258E-1 1.031E+0 1.523E-1 1.254E+0 2.654E-1 1.078E+0 1.483E-1
tauD_m [ms] 2.933E+0 2.034E+0 4.185E+1 4.152E+1 1.806E+0 2.725E+0 2.055E+0 1.294E+0 3.189E+1 6.329E+1 4.091E+0 6.596E+0
D_m [mum^2/s] 9.102E+0 3.985E+0 3.852E+0 1.343E+0 2.001E+1 1.253E+1 1.522E+1 1.573E+1 5.447E+0 2.061E+0 1.017E+1 5.446E+0
R [1/s] 9.096E+1 7.164E+1 4.286E+1 5.608E+1
CPM [kHz] 1.263E+0 2.282E-1 2.869E-1 2.192E-1 5.310E-1 3.960E-1 2.349E-1 1.089E-1
CPM_corr [kHz] 1.281E+0 2.317E-1 3.645E-1 2.645E-1 7.996E-1 5.248E-1 1.041E+0 3.042E+0
Int [kHz] 3.505E+2 3.412E+2 1.433E+2 3.777E+1 1.454E+1 3.694E+1 7.659E+1 4.204E+1
Int_corr [kHz] 3.699E+2 3.583E+2 2.346E+2 6.775E+1 2.650E+1 1.688E+2 1.478E+2 1.243E+2
# samples 118 118 47 47 42 42 187 187 18 18 10 10
# cells 69

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 1905.315 - sd = 2122.214Int_corr [kHz] - Ch1 - Nuc : ave = 369.910 - sd = 358.261r = 0.984 - r^2 = 0.969
f_1 - Ch1 - Nuc : ave = 0.859 - sd = 0.163D_1 [mum^2/s] - Ch1 - Nuc : ave = 11.072 - sd = 6.037r = 0.188 - r^2 = 0.035
f_2 - Ch1 - Nuc : ave = 0.141 - sd = 0.163D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.921 - sd = 0.549r = -0.403 - r^2 = 0.162
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.281 - sd = 0.232D_m [mum^2/s] - Ch1 - Nuc : ave = 9.102 - sd = 3.985r = 0.256 - r^2 = 0.066
c_corr [nM] - Ch1 - Nuc : ave = 1905.315 - sd = 2122.214ratioG_corr - Cross - Nuc : ave = 0.032 - sd = 0.043r = 0.016 - r^2 = 0.000
c_corr [nM] - Ch1 - Cyto : ave = 179.885 - sd = 418.116Int_corr [kHz] - Ch1 - Cyto : ave = 26.495 - sd = 168.764r = 0.689 - r^2 = 0.475
f_1 - Ch1 - Cyto : ave = 0.884 - sd = 0.101D_1 [mum^2/s] - Ch1 - Cyto : ave = 17.495 - sd = 21.192r = -0.380 - r^2 = 0.144
f_2 - Ch1 - Cyto : ave = 0.116 - sd = 0.101D_2 [mum^2/s] - Ch1 - Cyto : ave = 1.038 - sd = 0.597r = 0.334 - r^2 = 0.112
CPM_corr [kHz] - Ch1 - Cyto : ave = 0.800 - sd = 0.525D_m [mum^2/s] - Ch1 - Cyto : ave = 15.215 - sd = 15.731r = 0.280 - r^2 = 0.078