Automated F(C)CS analysis of clone # 0592

Created on 12/08/2014
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Gene and protein information

Clone # 0592
Gene symbol: RBM22
Description: RNA binding motif protein 22
Molecular weight (with FP): 73.9 kDa
Localisation: granular nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
0592_img_ch1.png0592_img_ch2.png


Point 1, intensity traces and correlation functions
0592_itr_1.png0592_cor_1.png

Point 2, intensity traces and correlation functions
0592_itr_2.png0592_cor_2.png

Point 3, intensity traces and correlation functions
0592_itr_3.png0592_cor_3.png

Point 4, intensity traces and correlation functions
0592_itr_4.png0592_cor_4.png

Point 5, intensity traces and correlation functions
0592_itr_5.png0592_cor_5.png

Point 6, intensity traces and correlation functions
0592_itr_6.png0592_cor_6.png

Sessions

1 20110915\U01--V00--0592
2 20110916\U01--V00--0592
3 20110922\U01--V00--0592
4 20111011\U01--V00--0592

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 4.520E+1 2.753E+1 1.083E+3 4.696E+2 3.915E+0 4.366E+0 4.058E+1 2.566E+1 1.695E+2 1.831E+2 4.203E+1 3.179E+1
N_corr 4.646E+1 2.920E+1 1.668E+3 8.006E+2 4.275E+0 4.866E+0 4.281E+1 2.765E+1 1.676E+2 3.958E+2 4.756E+1 3.353E+1
c [nM] 2.811E+2 1.712E+2 5.025E+3 2.178E+3 2.013E+1 2.244E+1 2.524E+2 1.596E+2 7.864E+2 8.495E+2 2.161E+2 1.634E+2
c_corr [nM] 2.889E+2 1.816E+2 7.737E+3 3.714E+3 2.198E+1 2.502E+1 2.662E+2 1.720E+2 7.776E+2 1.836E+3 2.445E+2 1.724E+2
ratioG 5.737E-2 3.817E-2 6.257E-1 2.659E-1
ratioG_corr 1.053E-2 1.928E-2 1.217E-1 1.197E-1
f_1 9.453E-1 5.012E-2 4.841E-1 1.863E-1 9.864E-1 3.770E-2 9.317E-1 5.024E-2 9.731E-1 8.117E-2 9.599E-1 3.818E-2
tauD_1 [ms] 4.238E-1 2.314E-1 1.868E+0 8.939E-2 2.738E-1 1.647E-1 3.487E-1 1.920E-1 7.537E-1 6.216E-1 1.921E-1 6.166E-2
D_1 [mum^2/s] 3.115E+1 1.274E+1 6.912E+0 3.198E-1 5.987E+1 3.205E+1 3.554E+1 1.167E+1 3.735E+1 3.251E+1 6.915E+1 2.298E+1
alpha_1 1.018E+0 7.306E-2 9.443E-1 3.681E-2 1.005E+0 2.024E-1 1.022E+0 1.043E-1 8.916E-1 2.200E-1 1.013E+0 2.002E-1
f_2 5.467E-2 5.012E-2 5.159E-1 1.863E-1 1.359E-2 3.770E-2 6.831E-2 5.024E-2 2.690E-2 8.117E-2 4.012E-2 3.818E-2
tauD_2 [ms] 7.056E+0 3.207E+0 7.983E+1 9.256E+1 4.083E+1 2.404E+1 1.163E+1 1.321E+1 1.148E+1 1.486E+1 1.200E+1 7.499E+0
D_2 [mum^2/s] 1.726E+0 5.740E-1 3.936E-1 4.021E-1 3.908E-1 2.136E-1 1.491E+0 7.658E-1 2.132E+0 8.663E-1 1.369E+0 7.063E-1
alpha_2 1.089E+0 1.091E-1 1.319E+0 3.289E-1 1.070E+0 5.368E-2 1.068E+0 1.396E-1 1.065E+0 1.927E-1 1.074E+0 9.195E-3
tauD_m [ms] 8.686E-1 7.820E-1 4.791E+1 7.057E+1 1.151E+0 3.989E+0 1.280E+0 1.724E+0 2.066E+0 4.243E+0 5.214E-1 3.679E-1
D_m [mum^2/s] 2.973E+1 1.280E+1 3.527E+0 1.229E+0 5.927E+1 3.229E+1 3.337E+1 1.134E+1 3.717E+1 3.269E+1 6.679E+1 2.377E+1
R [1/s] 2.080E+2 8.387E+1 5.252E+1 1.577E+2
CPM [kHz] 1.316E+0 1.746E-1 2.919E-1 2.395E-1 1.231E+0 4.252E-1 1.463E-1 6.598E-2
CPM_corr [kHz] 1.373E+0 1.827E-1 3.175E-1 2.619E-1 1.304E+0 4.179E-1 7.019E-1 3.914E-1
Int [kHz] 6.999E+1 4.327E+1 1.684E+2 4.745E+1 5.894E+1 4.311E+1 3.215E+1 4.223E+1
Int_corr [kHz] 7.387E+1 4.558E+1 2.760E+2 8.628E+1 6.413E+1 4.536E+1 4.910E+1 8.084E+1
# samples 97 97 69 69 48 48 104 104 19 19 17 17
# cells 41

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 288.928 - sd = 181.596Int_corr [kHz] - Ch1 - Nuc : ave = 73.874 - sd = 45.584r = 0.976 - r^2 = 0.952
f_1 - Ch1 - Nuc : ave = 0.945 - sd = 0.050D_1 [mum^2/s] - Ch1 - Nuc : ave = 31.152 - sd = 12.738r = 0.315 - r^2 = 0.099
f_2 - Ch1 - Nuc : ave = 0.055 - sd = 0.050D_2 [mum^2/s] - Ch1 - Nuc : ave = 1.726 - sd = 0.574r = -0.183 - r^2 = 0.034
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.373 - sd = 0.183D_m [mum^2/s] - Ch1 - Nuc : ave = 29.734 - sd = 12.805r = 0.043 - r^2 = 0.002
c_corr [nM] - Ch1 - Nuc : ave = 288.928 - sd = 181.596ratioG_corr - Cross - Nuc : ave = 0.011 - sd = 0.019r = 0.044 - r^2 = 0.002
c_corr [nM] - Ch1 - Cyto : ave = 266.216 - sd = 171.968Int_corr [kHz] - Ch1 - Cyto : ave = 64.127 - sd = 45.363r = 0.767 - r^2 = 0.588
f_1 - Ch1 - Cyto : ave = 0.932 - sd = 0.050D_1 [mum^2/s] - Ch1 - Cyto : ave = 35.538 - sd = 11.665r = 0.194 - r^2 = 0.038
f_2 - Ch1 - Cyto : ave = 0.068 - sd = 0.050D_2 [mum^2/s] - Ch1 - Cyto : ave = 1.491 - sd = 0.766r = -0.355 - r^2 = 0.126
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.304 - sd = 0.418D_m [mum^2/s] - Ch1 - Cyto : ave = 33.372 - sd = 11.340r = 0.145 - r^2 = 0.021