Automated F(C)CS analysis of clone # 0528

Created on 12/08/2014
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Gene and protein information

Clone # 0528
Gene symbol: TFIP11
Description: tuftelin interacting protein 11
Molecular weight (with FP): 123.8 kDa
Localisation: homogeneous nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
0528_img_ch1.png0528_img_ch2.png


Point 1, intensity traces and correlation functions
0528_itr_1.png0528_cor_1.png

Point 2, intensity traces and correlation functions
0528_itr_2.png0528_cor_2.png

Point 3, intensity traces and correlation functions
0528_itr_3.png0528_cor_3.png

Point 4, intensity traces and correlation functions
0528_itr_4.png0528_cor_4.png

Point 5, intensity traces and correlation functions
0528_itr_5.png0528_cor_5.png

Point 6, intensity traces and correlation functions
0528_itr_6.png0528_cor_6.png

Sessions

1 20111125\U02--V00--0528
2 20111209\U01--V00--0528
3 20120131\U01--V00--0528
4 20120208\U00--V00--0528
5 20120214\U00--V00--0528
6 20120308\U01--V00--0528

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.118E+2 5.893E+1 1.295E+3 5.474E+2 1.340E+1 1.064E+1 4.632E+1 4.151E+1 1.660E+2 1.861E+2 2.153E+1 1.936E+1
N_corr 1.175E+2 6.398E+1 1.960E+3 9.761E+2 1.483E+1 1.181E+1 4.896E+1 5.816E+1 1.383E+2 2.868E+2 2.641E+1 2.502E+1
c [nM] 6.951E+2 3.665E+2 6.008E+3 2.539E+3 6.889E+1 5.468E+1 2.881E+2 2.581E+2 7.699E+2 8.632E+2 1.107E+2 9.953E+1
c_corr [nM] 7.307E+2 3.979E+2 9.091E+3 4.528E+3 7.623E+1 6.071E+1 3.045E+2 3.617E+2 6.414E+2 1.330E+3 1.358E+2 1.286E+2
ratioG 9.532E-2 4.469E-2 4.876E-1 1.940E-1
ratioG_corr 2.715E-3 2.718E-2 8.717E-2 5.047E-2
f_1 8.980E-1 7.438E-2 3.703E-1 2.159E-1 9.817E-1 5.365E-2 9.099E-1 6.579E-2 8.190E-1 2.608E-1 9.715E-1 5.177E-2
tauD_1 [ms] 1.656E+0 6.374E-1 1.943E+0 6.186E-1 9.256E-1 6.014E-1 1.424E+0 6.518E-1 1.859E+0 3.597E-1 1.001E+0 6.827E-1
D_1 [mum^2/s] 7.461E+0 4.365E+0 6.884E+0 1.139E+0 2.223E+1 2.091E+1 8.773E+0 3.982E+0 8.534E+0 1.352E+1 1.582E+1 9.002E+0
alpha_1 9.515E-1 9.861E-2 9.238E-1 3.466E-2 8.859E-1 2.057E-1 9.144E-1 1.066E-1 9.313E-1 7.716E-2 7.738E-1 1.436E-1
f_2 1.020E-1 7.438E-2 6.297E-1 2.159E-1 1.835E-2 5.365E-2 9.012E-2 6.579E-2 1.810E-1 2.608E-1 2.851E-2 5.177E-2
tauD_2 [ms] 2.662E+1 1.677E+1 7.214E+1 7.595E+1 6.940E+1 5.661E+1 2.851E+1 2.238E+1 4.044E+1 4.613E+1 2.767E+1 1.223E+1
D_2 [mum^2/s] 5.043E-1 2.035E-1 4.926E-1 5.526E-1 2.411E-1 1.084E-1 4.826E-1 1.902E-1 9.623E-1 1.787E+0 5.032E-1 1.752E-1
alpha_2 1.273E+0 2.609E-1 1.236E+0 2.443E-1 1.074E+0 7.150E-2 1.213E+0 2.275E-1 1.169E+0 2.559E-1 1.117E+0 1.356E-1
tauD_m [ms] 4.522E+0 4.059E+0 4.591E+1 5.424E+1 3.504E+0 9.619E+0 3.745E+0 4.119E+0 9.302E+0 2.777E+1 1.704E+0 1.743E+0
D_m [mum^2/s] 6.742E+0 4.095E+0 2.837E+0 1.327E+0 2.173E+1 1.997E+1 7.935E+0 3.338E+0 7.260E+0 1.061E+1 1.534E+1 8.699E+0
R [1/s] 2.846E+1 2.357E+1 1.669E+1 2.209E+1
CPM [kHz] 1.295E+0 4.559E-1 3.453E-1 3.105E-1 1.328E+0 5.383E-1 1.658E-1 1.202E-1
CPM_corr [kHz] 1.319E+0 4.506E-1 4.029E-1 3.631E-1 1.414E+0 5.702E-1 4.239E-1 1.704E+0
Int [kHz] 1.615E+2 1.059E+2 1.954E+2 5.299E+1 5.507E+1 3.668E+1 2.770E+1 3.600E+1
Int_corr [kHz] 1.716E+2 1.122E+2 3.358E+2 1.079E+2 5.982E+1 4.170E+1 3.697E+1 5.674E+1
# samples 244 244 160 160 135 135 226 226 81 81 77 77
# cells 85

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 730.650 - sd = 397.891Int_corr [kHz] - Ch1 - Nuc : ave = 171.554 - sd = 112.211r = 0.907 - r^2 = 0.822
f_1 - Ch1 - Nuc : ave = 0.898 - sd = 0.074D_1 [mum^2/s] - Ch1 - Nuc : ave = 7.461 - sd = 4.365r = 0.061 - r^2 = 0.004
f_2 - Ch1 - Nuc : ave = 0.102 - sd = 0.074D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.504 - sd = 0.203r = -0.058 - r^2 = 0.003
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.319 - sd = 0.451D_m [mum^2/s] - Ch1 - Nuc : ave = 6.742 - sd = 4.095r = 0.168 - r^2 = 0.028
c_corr [nM] - Ch1 - Nuc : ave = 730.650 - sd = 397.891ratioG_corr - Cross - Nuc : ave = 0.003 - sd = 0.027r = -0.058 - r^2 = 0.003
c_corr [nM] - Ch1 - Cyto : ave = 304.493 - sd = 361.705Int_corr [kHz] - Ch1 - Cyto : ave = 59.824 - sd = 41.698r = 0.576 - r^2 = 0.332
f_1 - Ch1 - Cyto : ave = 0.910 - sd = 0.066D_1 [mum^2/s] - Ch1 - Cyto : ave = 8.773 - sd = 3.982r = -0.575 - r^2 = 0.330
f_2 - Ch1 - Cyto : ave = 0.090 - sd = 0.066D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.483 - sd = 0.190r = 0.342 - r^2 = 0.117
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.414 - sd = 0.570D_m [mum^2/s] - Ch1 - Cyto : ave = 7.935 - sd = 3.338r = 0.209 - r^2 = 0.044