Automated F(C)CS analysis of clone # 0421

Created on 12/08/2014
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Gene and protein information

Clone # 0421
Gene symbol: SAMHD1
Description: SAM domain and HD domain 1
Molecular weight (with FP): 99.2 kDa
Localisation: homogeneous nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
0421_img_ch1.png0421_img_ch2.png


Point 1, intensity traces and correlation functions
0421_itr_1.png0421_cor_1.png

Point 2, intensity traces and correlation functions
0421_itr_2.png0421_cor_2.png

Point 3, intensity traces and correlation functions
0421_itr_3.png0421_cor_3.png

Point 4, intensity traces and correlation functions
0421_itr_4.png0421_cor_4.png

Point 5, intensity traces and correlation functions
0421_itr_5.png0421_cor_5.png

Point 6, intensity traces and correlation functions
0421_itr_6.png0421_cor_6.png

Sessions

1 20111012\U01--V00--0421
2 20111013\U01--V00--0421
3 20111018\U01--V00--0421
4 20111108\U01--V00--0421
5 20111109\U03--V01--0421
6 20111110\U01--V00--0421

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.137E+2 5.744E+1 1.112E+3 5.956E+2 1.278E+1 1.529E+1 5.193E+1 3.803E+1 1.776E+2 1.656E+2 2.491E+1 2.798E+1
N_corr 1.258E+2 7.986E+1 1.577E+3 1.032E+3 2.051E+1 5.898E+1 5.760E+1 4.893E+1 1.909E+2 3.244E+2 3.548E+1 6.113E+1
c [nM] 7.070E+2 3.572E+2 5.156E+3 2.763E+3 6.572E+1 7.860E+1 3.229E+2 2.365E+2 8.239E+2 7.681E+2 1.280E+2 1.438E+2
c_corr [nM] 7.825E+2 4.967E+2 7.315E+3 4.787E+3 1.054E+2 3.032E+2 3.582E+2 3.043E+2 8.854E+2 1.505E+3 1.824E+2 3.143E+2
ratioG 9.799E-2 5.503E-2 5.094E-1 2.244E-1
ratioG_corr 1.402E-2 5.812E-2 1.169E-1 1.784E-1
f_1 9.200E-1 7.261E-2 4.519E-1 2.315E-1 9.835E-1 4.432E-2 9.208E-1 7.225E-2 8.143E-1 2.278E-1 9.612E-1 5.893E-2
tauD_1 [ms] 1.809E+0 9.940E-1 1.860E+0 4.441E-16 1.052E+0 7.965E-1 1.667E+0 1.412E+0 1.860E+0 0.000E+0 1.006E+0 5.665E-1
D_1 [mum^2/s] 8.538E+0 8.342E+0 6.926E+0 8.882E-16 2.403E+1 2.835E+1 9.142E+0 9.168E+0 6.926E+0 0.000E+0 1.560E+1 9.505E+0
alpha_1 9.534E-1 1.564E-1 9.200E-1 3.331E-16 9.151E-1 2.207E-1 8.935E-1 1.490E-1 9.200E-1 1.110E-16 8.198E-1 1.854E-1
f_2 8.001E-2 7.261E-2 5.481E-1 2.315E-1 1.651E-2 4.432E-2 7.923E-2 7.225E-2 1.857E-1 2.278E-1 3.883E-2 5.893E-2
tauD_2 [ms] 2.909E+1 2.184E+1 7.860E+1 9.121E+1 7.636E+1 7.413E+1 3.643E+1 3.620E+1 6.075E+1 1.036E+2 3.571E+1 2.814E+1
D_2 [mum^2/s] 5.550E-1 4.290E-1 5.269E-1 6.257E-1 2.356E-1 1.616E-1 4.465E-1 2.489E-1 7.144E-1 9.912E-1 4.461E-1 1.816E-1
alpha_2 1.186E+0 2.122E-1 1.267E+0 3.205E-1 1.075E+0 9.526E-2 1.155E+0 1.867E-1 1.132E+0 1.992E-1 1.159E+0 1.495E-1
tauD_m [ms] 4.384E+0 4.222E+0 4.173E+1 5.642E+1 3.068E+0 9.405E+0 4.373E+0 5.560E+0 1.162E+1 2.202E+1 2.093E+0 2.206E+0
D_m [mum^2/s] 7.993E+0 8.271E+0 3.477E+0 1.380E+0 2.385E+1 2.833E+1 8.254E+0 7.313E+0 5.882E+0 1.290E+0 1.501E+1 9.326E+0
R [1/s] 3.314E+1 4.744E+1 1.837E+1 2.168E+1
CPM [kHz] 1.277E+0 5.108E-1 3.339E-1 3.333E-1 1.150E+0 5.327E-1 2.172E-1 2.174E-1
CPM_corr [kHz] 1.298E+0 5.117E-1 3.943E-1 4.027E-1 1.205E+0 5.324E-1 6.133E-1 3.473E-1
Int [kHz] 1.494E+2 8.754E+1 1.819E+2 6.282E+1 5.434E+1 3.285E+1 4.051E+1 4.918E+1
Int_corr [kHz] 1.683E+2 1.261E+2 2.928E+2 1.243E+2 6.132E+1 3.789E+1 6.568E+1 9.470E+1
# samples 217 217 172 172 146 146 167 167 42 42 35 35
# cells 94

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 782.549 - sd = 496.661Int_corr [kHz] - Ch1 - Nuc : ave = 168.251 - sd = 126.112r = 0.728 - r^2 = 0.530
f_1 - Ch1 - Nuc : ave = 0.920 - sd = 0.073D_1 [mum^2/s] - Ch1 - Nuc : ave = 8.538 - sd = 8.342r = 0.034 - r^2 = 0.001
f_2 - Ch1 - Nuc : ave = 0.080 - sd = 0.073D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.555 - sd = 0.429r = -0.287 - r^2 = 0.083
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.298 - sd = 0.512D_m [mum^2/s] - Ch1 - Nuc : ave = 7.993 - sd = 8.271r = 0.157 - r^2 = 0.025
c_corr [nM] - Ch1 - Nuc : ave = 782.549 - sd = 496.661ratioG_corr - Cross - Nuc : ave = 0.014 - sd = 0.058r = 0.337 - r^2 = 0.114
c_corr [nM] - Ch1 - Cyto : ave = 358.234 - sd = 304.298Int_corr [kHz] - Ch1 - Cyto : ave = 61.322 - sd = 37.888r = 0.551 - r^2 = 0.304
f_1 - Ch1 - Cyto : ave = 0.921 - sd = 0.072D_1 [mum^2/s] - Ch1 - Cyto : ave = 9.142 - sd = 9.168r = -0.250 - r^2 = 0.063
f_2 - Ch1 - Cyto : ave = 0.079 - sd = 0.072D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.446 - sd = 0.249r = 0.164 - r^2 = 0.027
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.205 - sd = 0.532D_m [mum^2/s] - Ch1 - Cyto : ave = 8.254 - sd = 7.313r = 0.260 - r^2 = 0.068